oriTDB
The information of the oriT region
oriTDB accession number 100192
Name oriT_pUB110  insolico
Sequence Completeness core
oriT length 38 nt
oriT sequence CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC
IR (inverted repeat)[2-8] [13-19] nt  (ACTTTAT..ATAAAGT)
Location of nic site [27-28] nt
Conserved sequence flanking the
  nic site 		TAGTGTG|TTAT
Note _
L:2-8;R:13-19;D:27-28
Visualization of oriT structure
Reference
[1] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]
I. Information of Relaxase
ID 185
Name Mob_pUB110 insolico
GenBank accession number NP_040431
Family MOBV
Length 420 aa
UniProt ID Q9KJC8
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 1.70E-73, Aligned region: 1..193
Note relaxase
Protein sequence [Download] MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE
IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA
YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG
SDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPT
GEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYK
KLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLK
ERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 263 _ insolico _ _ _
 N/A
Information of plasmid
ID 185
Plasmid name pUB110
GenBank accession number NC_001384.1
Genome size 4548 bp
Coordinate of oriT  [Strand] 1144..1181 [-]
Drug resistance insolico neomycin resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Staphylococcus aureus [1280]
Reference
[1] McKenzie T et al (1986) The nucleotide sequence of pUB110: some salient features in relation to replication and its regulation. Plasmid. 15(2):93-103. [PMID:3010356]

Microbial Bioinformatics Group in MML, SJTU