oriTDB

The information of the oriT region

oriTDB accession number	100192
Name	oriT_pUB110
Sequence Completeness	core
oriT length	38 nt
oriT sequence	CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC
IR (inverted repeat)	[2-8] [13-19] nt (ACTTTAT..ATAAAGT)
Location of nic site	[27-28] nt
Conserved sequence flanking the nic site	TAGTGTG\|TTAT
Note	_
	L:2-8;R:13-19;D:27-28

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

Reference

[1] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

I. Information of Relaxase

ID	185
Name	Mob_pUB110
GenBank accession number	NP_040431
Family	MOB_V
Length	420 aa
UniProt ID	Q9KJC8
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 1.70E-73, Aligned region: 1..193
Note	relaxase
Protein sequence [Download]	MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG SDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPT GEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYK KLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLK ERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	263	_	_	_	_

N/A

Information of plasmid

ID	185
Plasmid name	pUB110
GenBank accession number	NC_001384.1
Genome size	4548 bp
Coordinate of oriT [Strand]	1144..1181 [-]
Drug resistance	neomycin resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus [1280]

Reference

[1] McKenzie T et al (1986) The nucleotide sequence of pUB110: some salient features in relation to replication and its regulation. Plasmid. 15(2):93-103. [PMID:3010356]