oriTDB
The information of the oriT region
oriTDB accession number 100190
Name oriT_pIP1714  insolico
Sequence Completeness core
oriT length 38 nt
oriT sequence CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC
IR (inverted repeat)[2-8] [13-19] nt  (ACTTTAT..ATAAAGT)
Location of nic site [27-28] nt
Conserved sequence flanking the
  nic site
TAGTGTG|TTAT
Note _
L:2-8;R:13-19;D:27-28
Visualization of oriT structure
Reference
[1] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]
I. Information of Relaxase
ID 183
Name Mob/Pre_pIP1714 insolico
GenBank accession number AAC61672
Family MOBV
Length 420 aa
UniProt ID O87277
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 1.70E-73, Aligned region: 1..193
Note relaxase
Protein sequence [Download] MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE
IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA
YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG
SDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPT
GEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYK
KLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLK
ERTDRLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 261 _ insolico _ _ _
 N/A
Information of plasmid
ID 183
Plasmid name pIP1714
GenBank accession number AF015628.1
Genome size 4978 bp
Coordinate of oriT  [Strand] 2356..2393 [+]
Drug resistance experimental resistance to streptogramins A and B
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Staphylococcus cohnii BM10711 [29382]
Reference
[1] Allignet J et al (1998) Characterization of a staphylococcal plasmid related to pUB110 and carrying two novel genes, vatC and vgbB, encoding resistance to streptogramins A and B and similar antibiotics. Antimicrob Agents Chemother. 42(7):1794-8. [PMID:9661023]