oriTDB

The information of the oriT region

oriTDB accession number	100188
Name	oriT_pGR71
Sequence Completeness	core
oriT length	38 nt
oriT sequence	CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC
IR (inverted repeat)	[2-8] [13-19] nt (ACTTTAT..ATAAAGT)
Location of nic site	[27-28] nt
Conserved sequence flanking the nic site	TAGTGTG\|TTAT
Note	_
	L:2-8;R:13-19;D:27-28

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

Reference

[1] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

I. Information of Relaxase

ID	181
Name	Mob_pGR71
GenBank accession number	CAA33525
Family	MOB_V
Length	313 aa
UniProt ID	Q45626
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 8.10E-74, Aligned region: 1..193
Note	relaxase
Protein sequence [Download]	MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG SDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPT GEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYK KLSFVSFDEIFRS

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	259	_	_	_	_

N/A

Information of plasmid

ID	181
Plasmid name	pGR71
GenBank accession number	X15503.1
Genome size	_
Coordinate of oriT [Strand]	_
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bacillus subtilis [1396]

Reference
N/A