oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100187
Name	oriT_pSK41
Sequence Completeness	incomplete
oriT length	12 nt
oriT sequence	TAAGTGCGCCCT
IR (inverted repeat)	_
Location of nic site	[10-11] nt
Conserved sequence flanking the nic site	TAAGTGCGCC\|CT
Note	_
	D:10-11

Visualization of oriT structure

Reference

[1] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

[2] Firth N et al (1993) Analysis of a transfer region from the staphylococcal conjugative plasmid pSK41. Gene. 136(1-2):13-25. [PMID:8293996]

I. Information of Relaxase

ID	180
Name	Nes_pSK41
GenBank accession number	NP_863609
Family	MOB_Q
Length	665 aa
UniProt ID	O87361
PDB ID	4HTE
Pfam	MobA_MobL [PF03389.14], Evalue: 7.70E-55, Aligned region: 17..218
Note	MobA/MobL family; pfam03389
Protein sequence [Download]	MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADD KFKDREYLWNKVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDL NIHKINEENPHAHLLCTLRGLDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKH LEKNGFSVRVSADSYKNQNIDLEPTKKEGWKARKFEDETGKKSSISKHNESIKKRNQQKI DKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYVTPINMYKENERLYDLKQKTS LITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSDDEKIFITRTI LNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAF NEVTDIKEKQQLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPN KNQHDKFVFGAIQKKLLSEYDFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILV DKETYNNYNRKQQAYGLINAGLDSMIFNFNEIFRERMPKYINHQYKGKNHSKQRHELKNK RGMHL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	258	_	_	_	_

N/A

Information of plasmid

ID	180
Plasmid name	pSK41
GenBank accession number	NC_005024.1
Genome size	46445 bp
Coordinate of oriT [Strand]	10212..10223 [+]
Drug resistance	resistance to bleomycin, gentamicin, kanamycin, neomycin and tobramycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus [1280]

Reference

[1] Berg T et al (1998) Complete nucleotide sequence of pSK41: evolution of staphylococcal conjugative multiresistance plasmids. J Bacteriol. 180(17):4350-9. [PMID:9721269]