oriTDB
The information of the oriT region
oriTDB accession number 100185
Name oriT_pBBR1  experimental
Sequence Completeness intact
oriT length 52 nt
oriT sequence GGTCACGACTTTGCGAAGCAAAGTCTAGTGAGTATACTCAAGCATTGAGTGG
IR (inverted repeat)IR1: [3-14] [18-31] nt  (TCACGACTTTGC..GCAAAGTCTAGTGA)
IR2: [36-42] [43-50] nt  (ACTCAAG..CATTGAGT)
Location of nic site [32-33] nt
Conserved sequence flanking the
  nic site 		TAGTGAG|TAT
Note _
L:3-14;R:18-31;L:36-42;R:43-50;D:32-33
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Szpirer CY et al (2001) Mobilization function of the pBHR1 plasmid, a derivative of the broad-host-range plasmid pBBR1. J Bacteriol. 183(6):2101-10. [PMID:11222611]
I. Information of Relaxase
ID 178
Name Mob_pBBR1 experimental
GenBank accession number YP_009062863
Family MOBV
Length 329 aa
UniProt ID Q9Z5R6
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 1.40E-42, Aligned region: 1..186
Note involved in mobilization
Protein sequence [Download] MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHWAASSTDEAMGRLREL
LPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLADKYGADRIVTASIHRD
ETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVADLGLQRGIEGSKARHTR
IQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKRVETPEAVADRLTKAVRQG
YEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGPLNRDMQAKAAAIIKAVGEK
LLTEQREVQRQKQAQRQQERGRDRGGYSR
Reference
[1] Szpirer CY et al (2001) Mobilization function of the pBHR1 plasmid, a derivative of the broad-host-range plasmid pBBR1. J Bacteriol. 183(6):2101-10. [PMID:11222611]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 256 _ insolico _ _ _
 N/A
Information of plasmid
ID 178
Plasmid name pBBR1
GenBank accession number NC_025015.1
Genome size 2687 bp
Coordinate of oriT  [Strand] 867..918 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bordetella bronchiseptica S87 [518]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU