oriTDB

The information of the oriT region

oriTDB accession number	100184
Name	oriT_pVA380-1
Sequence Completeness	core
oriT length	36 nt
oriT sequence	ACCTTTACTATGTAAAGGCTAGTACGTTACACTTTA
IR (inverted repeat)	[2-8] [12-18] nt (CCTTTAC..GTAAAGG)
Location of nic site	[26-27] nt
Conserved sequence flanking the nic site	TAGTACG\|TTA
Note	_
	L:2-8;R:12-18;D:26-27

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ACCTTTACTATGTAAAGGCTAGTACGTTACACTTTA

Reference

[1] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

I. Information of Relaxase

ID	177
Name	Mob_pVA380
GenBank accession number	AAA19677
Family	MOB_V
Length	430 aa
UniProt ID	Q54160
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 4.90E-73, Aligned region: 1..195
Note	relaxase
Protein sequence [Download]	MSYMVARMQKMKAGNLGGAFKHNERVFETHSNKDIDPSRSHLNYELTDRDRSVSYEKQIK DYVNENKVSNTRAIRKDAVLCDEWIITSDKQFFEQLDEEQTRAFFETAKNYFAENYGESN IAYASVHLDESTPHMHMGVVPFENGKLSSKSMFDREELKHIQEDLPRYMSDHGFELERGK LNSEAKHKTVAEFKRAMADMELKEELLEKYHAPLFVDERTGELNNDTEAFWHEKEFADMF EVQSPIRETTNREKMDWLRKQYQEELKKLESSKKPLEDDLSHLEELLDEKTKEYIKIDSE TSERLSELSKAEGYINTLENYSKSLEAKIERLEREGLKLEKLKTQIADLKIMSEKELAAI TPKKGVFGKEYVELTKEQFEEFKGLIYRSRNLVHQKELENEQLRRIVPLRRSKRFEASWN ELKKKVRERA

Reference

[1] LeBlanc DJ et al (1993) Identification and characterization of a mobilization gene in the streptococcal plasmid, pVA380-1. Plasmid. 30(3):296-302. [PMID:8302939]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	255	_	_	_	_

N/A

Information of plasmid

ID	177
Plasmid name	pVA380-1
GenBank accession number	M96957.1
Genome size	4.2 kb
Coordinate of oriT [Strand]	_
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Streptococcus sp. [1301]

Reference
N/A