oriTDB

The information of the oriT region

oriTDB accession number	100179
Name	oriT_pC221
Sequence Completeness	core
oriT length	53 nt
oriT sequence	GGGTATGGGATATAATCCCATCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACA ACGCTTGCCAAACCACA
IR (inverted repeat)	[12-19] [35-42] nt (GTATATTC..GAATATAC)
Location of nic site	[50-51] nt
Conserved sequence flanking the nic site	CGCTTG\|CCA
Note	_
	L:12-19;R:35-42;D:50-51

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGTATGGGATATAATCCCATCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACA

61

ACGCTTGCCAAACCACA

Reference

[1] Caryl JA et al (2006) Investigating the basis of substrate recognition in the pC221 relaxosome. Mol Microbiol . 60(5):1302-18. [PMID:16689804]

[2] Varsaki A et al (2009) Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins. J Bacteriol. 191(5):1446-55. [PMID:19114496]

I. Information of Relaxase

ID	172
Name	OrfA_pC221
GenBank accession number	NP_052695
Family	MOB_P
Length	315 aa
UniProt ID	P03865
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.20E-79, Aligned region: 8..239
Note	relaxase
Protein sequence [Download]	MATTKLGNTKSASRAINYAEKRAEEKSGLNCDVDYAKSAFKQTRALYGKEDGIQAHTVIQ SFKPGEVTPEQCNQLGLELAEKIAPNHQVAVYTHTDKDHYHNHIVINSVDLETGKKYQSN KKQRDLVKKENDNICREHGLSVTERGIAKMRYTQAEKGIVFDRDEYSWKDELRDLIENAK THTSNLETFSEHLEEKGVGVKLRGETISYKPENENKWVRGRTLGSEYEKGAIDHEHERHQ KQQREPEYADEFKINWDAVEQHTEQLKQRRVERAQETKQAHSKISSRDTRESENQRERAK GNNIRIERGDEGLSR

Reference

[1] Caryl JA et al (2006) Investigating the basis of substrate recognition in the pC221 relaxosome. Mol Microbiol . 60(5):1302-18. [PMID:16689804]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	248	_	_	_

N/A

Information of plasmid

ID	172
Plasmid name	pC221
GenBank accession number	NC_006977.1
Genome size	4555 bp
Coordinate of oriT [Strand]	3101..3153 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus [1280]

Reference
N/A