oriTDB
The information of the oriT region
oriTDB accession number 100179
Name oriT_pC221  insolico
Sequence Completeness core
oriT length 53 nt
oriT sequence GGGTATGGGATATAATCCCATCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACA ACGCTTGCCAAACCACA
IR (inverted repeat)[12-19] [35-42] nt  (GTATATTC..GAATATAC)
Location of nic site [50-51] nt
Conserved sequence flanking the
  nic site 		CGCTTG|CCA
Note _
L:12-19;R:35-42;D:50-51
Visualization of oriT structure
Reference
[1] Caryl JA et al (2006) Investigating the basis of substrate recognition in the pC221 relaxosome. Mol Microbiol . 60(5):1302-18. [PMID:16689804]
[2] Varsaki A et al (2009) Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins. J Bacteriol. 191(5):1446-55. [PMID:19114496]
I. Information of Relaxase
ID 172
Name OrfA_pC221 insolico
GenBank accession number NP_052695
Family MOBP
Length 315 aa
UniProt ID P03865
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 5.20E-79, Aligned region: 8..239
Note relaxase
Protein sequence [Download] MATTKLGNTKSASRAINYAEKRAEEKSGLNCDVDYAKSAFKQTRALYGKEDGIQAHTVIQ
SFKPGEVTPEQCNQLGLELAEKIAPNHQVAVYTHTDKDHYHNHIVINSVDLETGKKYQSN
KKQRDLVKKENDNICREHGLSVTERGIAKMRYTQAEKGIVFDRDEYSWKDELRDLIENAK
THTSNLETFSEHLEEKGVGVKLRGETISYKPENENKWVRGRTLGSEYEKGAIDHEHERHQ
KQQREPEYADEFKINWDAVEQHTEQLKQRRVERAQETKQAHSKISSRDTRESENQRERAK
GNNIRIERGDEGLSR
Reference
[1] Caryl JA et al (2006) Investigating the basis of substrate recognition in the pC221 relaxosome. Mol Microbiol . 60(5):1302-18. [PMID:16689804]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 248 _ insolico _ _
 N/A
Information of plasmid
ID 172
Plasmid name pC221
GenBank accession number NC_006977.1
Genome size 4555 bp
Coordinate of oriT  [Strand] 3101..3153 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Staphylococcus aureus [1280]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU