oriTDB
The information of the oriT region
oriTDB accession number 100178
Name oriT_pTF-FC2  insolico
Sequence Completeness core
oriT length 50 nt
oriT sequence CGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGTCATC CTGTATTGCTCAACCGCTCTACTATCATATCAGCAGTTTAGGCACACTAGATGCACTTTG CAACAAGCATAAAAGGCG
IR (inverted repeat)[1-18] [22-40] nt  (CCGTTGGAACCACGTGGC..GCCATGTGTGTTACAACGG)
Location of nic site [48-49] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note _
L:1-18;R:22-40;D:48-49
Visualization of oriT structure
Reference
[1] Rohrer J et al (1992) Sequence analysis and characterization of the mobilization region of a broad-host-range plasmid, pTF-FC2, isolated from Thiobacillus ferrooxidans. J Bacteriol. 174(19):6230-7. [PMID:1400173]
[2] van Zyl LJ et al (2003) Analysis of the mobilization region of the broad-host-range IncQ-like plasmid pTC-F14 and its ability to interact with a related plasmid, pTF-FC2. J Bacteriol. 185(20):6104-11. [PMID:14526022]
[3] Rawlings DE (2005) The evolution of pTF-FC2 and pTC-F14, two related plasmids of the IncQ-family. Plasmid. 53(2):137-47. [PMID:15737401]
I. Information of Relaxase
ID 171
Name MobA_pTF-FC2 insolico
GenBank accession number AAA27389
Family MOBP
Length 409 aa
UniProt ID P22898
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 2.20E-28, Aligned region: 5..217
Note relaxase
Protein sequence [Download] MIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTD
NLHLHLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELG
REHIDKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPIIKKAQTWEQLHRELAAKGMRY
EKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPQRQQVAQREPEPIKPDVPGW
KDYITGRKAHYSEKNADKLVQDKRQEQERKQLAEQQKARRDELMRGNWKGKGEVLNAMRS
VIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRI
EGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEEGGRGGGVSFVDKGKSID
Reference
[1] van Zyl LJ et al (2003) Analysis of the mobilization region of the broad-host-range IncQ-like plasmid pTC-F14 and its ability to interact with a related plasmid, pTF-FC2. J Bacteriol. 185(20):6104-11. [PMID:14526022]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 246 MobB_pTF-FC2 insolico B43256 106 aa relaxosome component
2 247 MobC_pTF-FC2 insolico AAA27390 118 aa relaxosome component
 N/A
Information of plasmid
ID 171
Plasmid name pTF-FC2
GenBank accession number M57717.1
Incompatibility group IncQ2
Genome size 12.2 kb
Coordinate of oriT  [Strand] _
Drug resistance _
Heavy-metal resistance insolico contains merA (mercury reductase) gene
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Thiobacillus ferrooxidans FC1 [920]
Reference
[1] Smith AS et al (1997) The poison-antidote stability system of the broad-host-range Thiobacillus ferrooxidans plasmid pTF-FC2. Mol Microbiol. 26(5):961-70. [PMID:9426133]
[2] Rohrer J et al (1992) Sequence analysis and characterization of the mobilization region of a broad-host-range plasmid, pTF-FC2, isolated from Thiobacillus ferrooxidans. J Bacteriol. 174(19):6230-7. [PMID:1400173]

Microbial Bioinformatics Group in MML, SJTU