oriTDB

The information of the oriT region

oriTDB accession number	100178
Name	oriT_pTF-FC2
Sequence Completeness	core
oriT length	50 nt
oriT sequence	CGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGTCATC CTGTATTGCTCAACCGCTCTACTATCATATCAGCAGTTTAGGCACACTAGATGCACTTTG CAACAAGCATAAAAGGCG
IR (inverted repeat)	[1-18] [22-40] nt (CCGTTGGAACCACGTGGC..GCCATGTGTGTTACAACGG)
Location of nic site	[48-49] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	_
	L:1-18;R:22-40;D:48-49

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGTCATC

61

CTGTATTGCTCAACCGCTCTACTATCATATCAGCAGTTTAGGCACACTAGATGCACTTTG

121

CAACAAGCATAAAAGGCG

Reference

[1] Rohrer J et al (1992) Sequence analysis and characterization of the mobilization region of a broad-host-range plasmid, pTF-FC2, isolated from Thiobacillus ferrooxidans. J Bacteriol. 174(19):6230-7. [PMID:1400173]

[2] van Zyl LJ et al (2003) Analysis of the mobilization region of the broad-host-range IncQ-like plasmid pTC-F14 and its ability to interact with a related plasmid, pTF-FC2. J Bacteriol. 185(20):6104-11. [PMID:14526022]

[3] Rawlings DE (2005) The evolution of pTF-FC2 and pTC-F14, two related plasmids of the IncQ-family. Plasmid. 53(2):137-47. [PMID:15737401]

I. Information of Relaxase

ID	171
Name	MobA_pTF-FC2
GenBank accession number	AAA27389
Family	MOB_P
Length	409 aa
UniProt ID	P22898
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.20E-28, Aligned region: 5..217
Note	relaxase
Protein sequence [Download]	MIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTD NLHLHLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELG REHIDKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPIIKKAQTWEQLHRELAAKGMRY EKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPQRQQVAQREPEPIKPDVPGW KDYITGRKAHYSEKNADKLVQDKRQEQERKQLAEQQKARRDELMRGNWKGKGEVLNAMRS VIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRI EGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEEGGRGGGVSFVDKGKSID

Reference

[1] van Zyl LJ et al (2003) Analysis of the mobilization region of the broad-host-range IncQ-like plasmid pTC-F14 and its ability to interact with a related plasmid, pTF-FC2. J Bacteriol. 185(20):6104-11. [PMID:14526022]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	246	MobB_pTF-FC2	B43256	106 aa	relaxosome component
2	247	MobC_pTF-FC2	AAA27390	118 aa	relaxosome component

N/A

Information of plasmid

ID	171
Plasmid name	pTF-FC2
GenBank accession number	M57717.1
Incompatibility group	IncQ2
Genome size	12.2 kb
Coordinate of oriT [Strand]	_
Drug resistance	_
Heavy-metal resistance	contains merA (mercury reductase) gene
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Thiobacillus ferrooxidans FC1 [920]

Reference

[1] Smith AS et al (1997) The poison-antidote stability system of the broad-host-range Thiobacillus ferrooxidans plasmid pTF-FC2. Mol Microbiol. 26(5):961-70. [PMID:9426133]

[2] Rohrer J et al (1992) Sequence analysis and characterization of the mobilization region of a broad-host-range plasmid, pTF-FC2, isolated from Thiobacillus ferrooxidans. J Bacteriol. 174(19):6230-7. [PMID:1400173]