oriTDB

The information of the oriT region

oriTDB accession number	100177
Name	oriT_R721
Sequence Completeness	core
oriT length	53 nt
oriT sequence	CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
IR (inverted repeat)	[5-21] [25-42] nt (CGATTGTAACATGACCG..CGGTCTTGTGTACAATCG)
Location of nic site	[50-51] nt
Conserved sequence flanking the nic site	TATCGTG\|C
Note	_
	L:5-21;R:25-42;D:50-51

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT

Reference

[1] Varsaki A et al (2009) Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins. J Bacteriol. 191(5):1446-55. [PMID:19114496]

[2] Kim SR et al (1992) Nucleotide sequence of the R721 shufflon. J Bacteriol. 174(21):7053-8. [PMID:1400257]

I. Information of Relaxase

ID	170
Name	NikB_R721
GenBank accession number	NP_065331
Family	MOB_P
Length	1272 aa
UniProt ID	Q9F553
PDB ID	_
Pfam
Note	relaxase
Protein sequence [Download]	MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTTDKIIDSIENKSQERYL HITLSFHESHVSNEVLKAVVDDYKKLLMNAYHPDEYSFYAEAHLPKIRHIQDNSTGELVE RKPHIHIVIPKVNLITEKFLNPVGDVTKGHTIEQLDAIQEFINNKYNLDSPKDYPRKDAD YGKIISRVKGDLYKEHHSELKGELLSRIENEKIENYSVFKDIVAEYGELRIRNAGKTNEY LAVKLPGDKKYTNLKSPLFRQNYIETRTLTLEKPTHKEIEKRLNAWLNKTSHEIKHIFNQ AEKTRELYKTLSPSQQIDFLQERIKEYDSREKLNERNSQQTSGRAGGYKSCPKKFARIRQ SEATVGLSRMPQRGMVYGINGFTRPDSVSVLSDISQRDLAKQLPQREHPGQDVRRDYDRQ FTESGIKSLERSSFLCETMFQTLNEAAEKNEITTMAEIRRNIDPVRFLSSAAERFNIIPA QHKIRTAKDGSPRFSVGNRNMNASDFLTKHINLAWKDAKSFLLEVYSQQLENTPYTRYPT YRRLTHHEARERLNSLNLSEKTLRNTIRFERGKLYNDLREMRRELKLIPREQRDIAVGVI VYKKLTTLERLSELDTEGRHIIRQYHADWHKDKDEMKALERLKSYLNFDEINAISADEPE LSLQKAVDSQRRLEEAKKINTKLKDLVMDKQDSRIVYRDQESEKPVFTDKGNFVVAGKNP SKEEIGIMLEYSREKFGGVLKLTGSEDFKKMCAEVAAEQDMKIILRPEQYQQMMLELKAE LQGNKFEQVETQENSQESESRIEKGDALKEQATEQEQATEQAQATEQEQATEQAQATEQA QATEQAQATEQAQATEQAQATEQAQDTEQAQATEQAQATEQAQATEQAQIAEQATSSYDP GVITRANILDSQMLSKGTNGEFGYLKSLDSDENEIWEVLGHVPGDSDDIFSVASFDNEND AKEFCKIVNELGIDRTQALIQEQLSSQQEQAAVQETYQESPEEILASISANEHMISGLEN FLVKDRSQFSSCNGDIVVEAEITRGEGGLYHLAVAGKHGLERGDAVARVDVTEQQFAAIT GKTPSEVLTGDQTSARVPVITGIHFSTRAIENVNKLEQQKDYVYFSTHEGLNAEIKDFSS LKDAIEWGRVECELHDLNKRDTVIYRVESEHISQGIDAVMKNAERVERHEIEKAQGRDCT PEDGKILEAIDRFEDKFRGEGLKFEREKAESDLLNHGFTREMAEDALGKQFVQAREDHQE SQQQRDDSQDMH

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	245	NikA_R721	NP_065330	113 aa	relaxosome component

N/A

Information of plasmid

ID	170
Plasmid name	R721
GenBank accession number	NC_002525.1
Incompatibility group	IncI2
Genome size	75582 bp
Coordinate of oriT [Strand]	29007..29059 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli K-12 [83333]

Reference
N/A