 oriTDB accession number | 100175 |
| Name | oriT_pTF1  |
Sequence Completeness  | core |
| oriT length | 42 nt |
| oriT sequence | GCACGGGTAATCTCGAAGAGATTACTCTAAGTGCGCCCTTGC |
| IR (inverted repeat) | [5-14] [18-27] nt (GGGTAATCTC..GAGATTACTC) |
| Location of nic site | [34-35] nt |
| Conserved sequence flanking the nic site | TAAGTGC|GCCCT |
| Note | _ |
Visualization of oriT structure (The oriT was characterized experimentally) Reference| [1] Drolet M et al (1992) Mobilization protein-DNA binding and divergent transcription at the transfer origin of the Thiobacillus ferrooxidans pTF1 plasmid. Mol Microbiol. 6(8):1061-71. [PMID:1584023] |
| [2] Cook DM et al (1992) The oriT region of the Agrobacterium tumefaciens Ti plasmid pTiC58 shares DNA sequence identity with the transfer origins of RSF1010 and RK2/RP4 and with T-region borders. J Bacteriol. 174(19):6238-46. [PMID:1400174] |
| [3] Parker C et al (2005) Elements in the co-evolution of relaxases and their origins of transfer. Plasmid . 53(2):113-8. [PMID:15737398] |
| ID | 168 |
| Name | MobL_pTF1  |
| GenBank accession number | CAA36927 |
| Family | MOBQ |
| Length | 378 aa |
| UniProt ID | P20085 |
| PDB ID | _ |
| Pfam | MobA_MobL [PF03389.14], Evalue: 2.30E-50, Aligned region: 17..244 |
| Note | relaxase |
| Protein sequence [Download] | MAIYHLSAKPISRSAGRSATGAAAYRAGVEITDERTGLVHDYTRKGGVLHSELILPGGGT ADRAEFWNGVEAHHKRGDAVLVREIEISLPTELTAEQRKALAVGYARALADRYGVAADVA LHAPRTVTDRELEKHPDQYHETDPKTGRRHNGNWHAHILLSACHVQPDGTLGKKAVELDP IHCQRAKIENLVDRERVRWGELANAALEHHGHAARIDHRSHAKRGIEAEPTRHLGPAAAG IERRTGEESRRRHDFEREAMERLARAQAQGVLERESRGLERSIFDLSGDMRAAFQERDRL REQQERAERERREQERAREAQRQQEKQQADKAASDQREAERILAQLLEKAPVVDRKTEKA IDGLFHDIARSRSRGRGR |
Reference| [1] Drolet M et al (1992) Mobilization protein-DNA binding and divergent transcription at the transfer origin of the Thiobacillus ferrooxidans pTF1 plasmid. Mol Microbiol. 6(8):1061-71. [PMID:1584023] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 241 | MobS_pTF1 | CAA36926 | 98 aa | _ |
| ID | 168 |
| Plasmid name | pTF1 |
| GenBank accession number | X52699.1 |
| Incompatibility group | IncQ1 |
| Genome size | 6.7 kb |
| Coordinate of oriT [Strand] | 1025..1066 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Acidithiobacillus ferrooxidans ATCC 33020 [1232575] |
Reference