oriTDB

The information of the oriT region

oriTDB accession number	100175
Name	oriT_pTF1
Sequence Completeness	core
oriT length	42 nt
oriT sequence	GCACGGGTAATCTCGAAGAGATTACTCTAAGTGCGCCCTTGC
IR (inverted repeat)	[5-14] [18-27] nt (GGGTAATCTC..GAGATTACTC)
Location of nic site	[34-35] nt
Conserved sequence flanking the nic site	TAAGTGC\|GCCCT
Note	_
	L:5-14;R:18-27;D:34-35

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GCACGGGTAATCTCGAAGAGATTACTCTAAGTGCGCCCTTGC

Reference

[1] Drolet M et al (1992) Mobilization protein-DNA binding and divergent transcription at the transfer origin of the Thiobacillus ferrooxidans pTF1 plasmid. Mol Microbiol. 6(8):1061-71. [PMID:1584023]

[2] Cook DM et al (1992) The oriT region of the Agrobacterium tumefaciens Ti plasmid pTiC58 shares DNA sequence identity with the transfer origins of RSF1010 and RK2/RP4 and with T-region borders. J Bacteriol. 174(19):6238-46. [PMID:1400174]

[3] Parker C et al (2005) Elements in the co-evolution of relaxases and their origins of transfer. Plasmid . 53(2):113-8. [PMID:15737398]

I. Information of Relaxase

ID	168
Name	MobL_pTF1
GenBank accession number	CAA36927
Family	MOB_Q
Length	378 aa
UniProt ID	P20085
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 2.30E-50, Aligned region: 17..244
Note	relaxase
Protein sequence [Download]	MAIYHLSAKPISRSAGRSATGAAAYRAGVEITDERTGLVHDYTRKGGVLHSELILPGGGT ADRAEFWNGVEAHHKRGDAVLVREIEISLPTELTAEQRKALAVGYARALADRYGVAADVA LHAPRTVTDRELEKHPDQYHETDPKTGRRHNGNWHAHILLSACHVQPDGTLGKKAVELDP IHCQRAKIENLVDRERVRWGELANAALEHHGHAARIDHRSHAKRGIEAEPTRHLGPAAAG IERRTGEESRRRHDFEREAMERLARAQAQGVLERESRGLERSIFDLSGDMRAAFQERDRL REQQERAERERREQERAREAQRQQEKQQADKAASDQREAERILAQLLEKAPVVDRKTEKA IDGLFHDIARSRSRGRGR

Reference

[1] Drolet M et al (1992) Mobilization protein-DNA binding and divergent transcription at the transfer origin of the Thiobacillus ferrooxidans pTF1 plasmid. Mol Microbiol. 6(8):1061-71. [PMID:1584023]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	241	MobS_pTF1	CAA36926	98 aa	_

N/A

Information of plasmid

ID	168
Plasmid name	pTF1
GenBank accession number	X52699.1
Incompatibility group	IncQ1
Genome size	6.7 kb
Coordinate of oriT [Strand]	1025..1066 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Acidithiobacillus ferrooxidans ATCC 33020 [1232575]

Reference
N/A