oriTDB
The information of the oriT region
oriTDB accession number 100174
Name oriT_pIP421  experimental
Sequence Completeness incomplete
oriT length 7 nt
oriT sequence CAAGCTC
IR (inverted repeat)_
Location of nic site _
Conserved sequence flanking the
  nic site
_
Note The oriT region was determined experimentally, but the putative nic site was not determined experimentally.
D:
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Trinh S et al (1997) Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis. J Bacteriol. 179(12):4071-4. [PMID:9190830]
I. Information of Relaxase
ID 167
Name Mob421_pIP421 insolico
GenBank accession number CAA71505
Family Other
Length 425 aa
UniProt ID P96200
PDB ID _
Pfam
Note A member of the Bacteroides mobilization protein family, which includes the MobA of pBI143, NBUs, and Tn4555
Protein sequence [Download] MAKTSINVRPCNIGSAERHNLRSKELDYIRPELSHRNEQWSEMKIADVLEDIREKYRQHT
GQSMQKKATPIREGVVVIEDGTTLRQLQDFAGRLEERFGIHTFQIYTHKDERASVWDGNA
ETWKPNYHAHMIFDWTDGETGRTRKLNKQDMAEMQTILAECLGMERGISSDRKLLSAIQY
KNMVEAEKAAQIEKECSQMEDERNAIEEKVKQAGQKLEDTQKKLKEAAAEIKVEKLKGAA
ADTGTALLKAGTTVIDVTRSLFNSGKVKRQEQEIAELKRENYELEMKSQNLQSNLRTANA
QNEREREATRRAVRTGEERLKPITNLFPCMENAKENIEELREMGVRDNDIRLLLTGKEIT
YSGFLYDRERRKKHQVKNVKINIAKSKAGNTTIWLNDFHFKEFFKQLWQKLQKVLGIDKG
RGFHL
Reference
[1] Trinh S et al (1997) Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis. J Bacteriol. 179(12):4071-4. [PMID:9190830]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 240 _ insolico
 N/A
Information of plasmid
ID 167
Plasmid name pIP421
GenBank accession number Y10480.1
Genome size 7.3 kb
Coordinate of oriT  [Strand] 513..519 [+]
Drug resistance insolico 5-nitroimidazole
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bacteroides fragilis BF-F239 [817]
Reference
[1] Trinh S et al (1997) Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis. J Bacteriol. 179(12):4071-4. [PMID:9190830]