oriTDB

The information of the oriT region

oriTDB accession number	100174
Name	oriT_pIP421
Sequence Completeness	incomplete
oriT length	7 nt
oriT sequence	CAAGCTC
IR (inverted repeat)	_
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	The oriT region was determined experimentally, but the putative nic site was not determined experimentally.
	D:

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CAAGCTC

Reference

[1] Trinh S et al (1997) Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis. J Bacteriol. 179(12):4071-4. [PMID:9190830]

I. Information of Relaxase

ID	167
Name	Mob_{421_pIP421}
GenBank accession number	CAA71505
Family	Other
Length	425 aa
UniProt ID	P96200
PDB ID	_
Pfam
Note	A member of the Bacteroides mobilization protein family, which includes the MobA of pBI143, NBUs, and Tn4555
Protein sequence [Download]	MAKTSINVRPCNIGSAERHNLRSKELDYIRPELSHRNEQWSEMKIADVLEDIREKYRQHT GQSMQKKATPIREGVVVIEDGTTLRQLQDFAGRLEERFGIHTFQIYTHKDERASVWDGNA ETWKPNYHAHMIFDWTDGETGRTRKLNKQDMAEMQTILAECLGMERGISSDRKLLSAIQY KNMVEAEKAAQIEKECSQMEDERNAIEEKVKQAGQKLEDTQKKLKEAAAEIKVEKLKGAA ADTGTALLKAGTTVIDVTRSLFNSGKVKRQEQEIAELKRENYELEMKSQNLQSNLRTANA QNEREREATRRAVRTGEERLKPITNLFPCMENAKENIEELREMGVRDNDIRLLLTGKEIT YSGFLYDRERRKKHQVKNVKINIAKSKAGNTTIWLNDFHFKEFFKQLWQKLQKVLGIDKG RGFHL

Reference

[1] Trinh S et al (1997) Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis. J Bacteriol. 179(12):4071-4. [PMID:9190830]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	240	_

N/A

Information of plasmid

ID	167
Plasmid name	pIP421
GenBank accession number	Y10480.1
Genome size	7.3 kb
Coordinate of oriT [Strand]	513..519 [+]
Drug resistance	5-nitroimidazole
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bacteroides fragilis BF-F239 [817]

Reference

[1] Trinh S et al (1997) Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis. J Bacteriol. 179(12):4071-4. [PMID:9190830]