oriTDB

The information of the oriT region

oriTDB accession number	100173
Name	oriT_pEC3
Sequence Completeness	incomplete
oriT length	8 nt
oriT sequence	CTGGCTTA
IR (inverted repeat)	_
Location of nic site	[4-5] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	_
	D:4-5

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTGGCTTA

Reference

[1] Nomura N et al (1996) Genetic organization of a DNA-processing region required for mobilization of a non-self-transmissible plasmid, pEC3, isolated from Erwinia carotovora subsp. carotovora. Gene. 170(1):57-62. [PMID:8621089]

I. Information of Relaxase

ID	166
Name	MobA_pEC3
GenBank accession number	BAA08130
Family	MOB_P
Length	498 aa
UniProt ID	Q47385
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.80E-16, Aligned region: 41..247
Note	relaxase
Protein sequence [Download]	MIVKFHPRGRGGGAGPVDYLLGKDRNRDGASVLQGKPEEVRELIDASPYAKKYTSGVLSF AEQDLPPGQREKLMASFERILMPGLDKDQYSVLWVEHQDKGRLELNFLVPNTELLTGKRL QPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSALPEAKQEAAQTITRGLLA LASSGELKTRQDVTEAVESAGFEVLRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL RAEIESARAEYRRDAESRIQRAREVCQSGTERKREENQRRYPRPRPEAVFSHEPAHERDA AHGQPDVADHRAGFRPADRVERGHSVVAGAADTRQLHDHPGAEEHAGDAVREEQRRATHD LWRAETPLREGEPGSGNVRRGFELDDTGGEIAHDGAGKTVVERIRAATAGLLEKAGRVGE RLRGMADDVWSYATGERSAERARHGLEQAGAEFERAAKPVVERLNVIEQRRQYEHSLQLQ KERGSAHQRHYSGRSMEL

Reference

[1] Nomura N et al (1996) Genetic organization of a DNA-processing region required for mobilization of a non-self-transmissible plasmid, pEC3, isolated from Erwinia carotovora subsp. carotovora. Gene. 170(1):57-62. [PMID:8621089]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	238	MobB_pEC3	BAA08131	161 aa	MbeB-like, N-term conserved region; pfam04837
2	239	MobC_pEC3	BAA08129	114 aa	Bacterial mobilisation protein (MobC); pfam05713

N/A

Information of plasmid

ID	166
Plasmid name	pEC3
GenBank accession number	D45188.1
Genome size	5.8 kb
Coordinate of oriT [Strand]	797..804 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Erwinia carotovora IFO3380 [554]

Reference
N/A