oriTDB

The information of the oriT region

oriTDB accession number	100169
Name	oriT_pBTK445
Sequence Completeness	core
oriT length	51 nt
oriT sequence	AGCCTTTAAAGCGAAAATAGGGTACTCCATGCTCGCTATATCATCCTGACA
IR (inverted repeat)	[17-25] [31-39] nt (ATAGGGTAC..GCTCGCTAT)
Location of nic site	[48-49] nt
Conserved sequence flanking the nic site	CATCCTG\|A
Note	IncP-like
	L:17-25;R:31-39;D:48-49

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGCCTTTAAAGCGAAAATAGGGTACTCCATGCTCGCTATATCATCCTGACA

Reference

[1] Dam B et al (2009) Conjugative Type 4 secretion system of a novel large plasmid from the chemoautotroph Tetrathiobacter kashmirensis and construction of shuttle vectors for Alcaligenaceae. Appl Environ Microbiol. 75(13):4362-73. [PMID:19411426]

N/A

Information of T4CP

ID	162
Name	VirD4_pBTK445
SecReT4 accesion number	_
GenBank accession number	ACD43639
Family	VirD4/TraG
Length	297
UniProt ID	B4YK42
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 2.80E-33, Aligned region: 101..277
Note	Type IV secretory pathway, VirD4 component,TraG/TraD family ATPase
Protein sequence [Download]	MFFMKKAMIWLAALVACTLVFAVIGQYVGSLVFIRFAKAPIDPGIYVLWDLYKNTQGGGI WSLWQYKVAVVFSVLIALLPIIAGIGAFFFRPQARITWFGQVRHARRDCEAGLLNDSHES PDIIIGKYKGKYLRWGGNQFAFLAAPPRSGKGVGIVIPNCLHYRDSLVVFDPKLENYEIT AGYREAHGQEVYLLHLSTDQFRSHRWNPLSYVKRDPDFAPGGAFSIAYILYPTSGNMSGN SIFFNEMAQKLFVGLCLYLIETENETGVIPSMPALLKLATPRRRGNLWPIGLKRQKK

Reference
N/A

Information of plasmid

ID	162
Plasmid name	pBTK445
GenBank accession number	EU585932.1
Incompatibility group	IncP
Genome size	24440 bp
Coordinate of oriT [Strand]	2952..3002 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Tetrathiobacter kashmirensis WGT [310575]

Reference
N/A