oriTDB

The information of the oriT region

oriTDB accession number	100168
Name	oriT2_pNZ4000
Sequence Completeness	intact
oriT length	416 nt
oriT sequence	AAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTAT TTATGGTTTT
IR (inverted repeat)	IR1: [91-100] [105-114] nt (CCCATTTTAT..ATAAAATGGG) IR2: [160-172] [176-188] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [196-208] [221-233] nt (CATTGTAATACAA..TTGTATTACAATG)
Location of nic site	[243-244] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	_
	L:91-100;R:105-114;L:160-172;R:176-188;L:196-208;R:221-233;D:243-244

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTAT

61

TTATGGTTTT

Reference

[1] van Kranenburg R et al (1998) Characterization of multiple regions involved in replication and mobilization of plasmid pNZ4000 coding for exopolysaccharide production in Lactococcus lactis. J Bacteriol. 180(20):5285-90. [PMID:9765557]

I. Information of Relaxase

ID	161
Name	MobA_pNZ4000
GenBank accession number	NP_053037
Family	MOB_P
Length	410 aa
UniProt ID	O54677
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.20E-74, Aligned region: 9..280
Note	relaxase
Protein sequence [Download]	MATIAKISNGASAASALNYALGQDRPMHEKTEQWLHDHQLERPVELTNCRAVAVGGTNGI DPFIAKEQFDVVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH ILEPPKERQTETEKELIAKNEYSYMNDLRERINKSLQDVSVSSYETFKERLSDNGVILSE RGQTFSYAFLDANNKQRRARETRLGSDFGKETILHELENRARQNEFSAVEQREPAITPLE RDTQQRESEIVSLEQAIEPRKSEARKRESKINRFIDTIKQFAGRVPELTQRVTRKLKQTK EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

Reference

[1] van Kranenburg R et al (1998) Characterization of multiple regions involved in replication and mobilization of plasmid pNZ4000 coding for exopolysaccharide production in Lactococcus lactis. J Bacteriol. 180(20):5285-90. [PMID:9765557]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	232	MobB_pNZ4000	NP_053038	207 aa	putative mobilization protein
2	233	MobC_pNZ4000	NP_053039	200 aa	putative mobilization protein

N/A

Information of plasmid

ID	161
Plasmid name	pNZ4000
GenBank accession number	NC_002137.1
Genome size	42810 bp
Coordinate of oriT [Strand]	28620..29037 [+]; 11149..11564 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis subsp. cremoris NIZO B40 [1359]

Reference
N/A