oriTDB

The information of the oriT region

oriTDB accession number	100166
Name	oriT_oriT_pKL1
Sequence Completeness	intact
oriT length	100 nt
oriT sequence	CGGGGTGTCGGGGTGAAGCCCTGACCAAGTGGTAATCGTATCGGCGTGCATGCGCGGTTA TACGATTACACATCCTGTCCCGATTTCTGAGGCGTTTTAA
IR (inverted repeat)	[7-15] [17-25] nt (GTCGGGGGT..AGCCCTGAC) [32-50] [51-69] nt (GTAATCGTATCGGCGTGCA..TGCGCGGTTATACGATTAC)
Location of nic site	[77-78] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	_
	L:7-15;R:17-25;L:32-50;R:51-69;D:77-78

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGGGGTGTCGGGGTGAAGCCCTGACCAAGTGGTAATCGTATCGGCGTGCATGCGCGGTTA

61

TACGATTACACATCCTGTCCCGATTTCTGAGGCGTTTTAA

Reference

[1] Srivastava P et al (2006) Characterization of broad host range cryptic plasmid pCR1 from Corynebacterium renale. Plasmid. 56(1):24-34. [PMID:16545871]

[2] Burian et al (1999) Replication Control of a Small Cryptic Plasmid of Escherichia coli. J Mol Biol. 294(1):49-65. [PMID:10556028]

N/A

Information of plasmid

ID	160
Plasmid name	pKL1
GenBank accession number	NC_002145.1
Genome size	1549 bp
Coordinate of oriT [Strand]	334..400 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli [562]

Reference
N/A