oriTDB

The information of the oriT region

oriTDB accession number	100165
Name	oriT_pMD136
Sequence Completeness	incomplete
oriT length	63 nt
oriT sequence	GTAAATTGATTGATTAAGCTCGTGAAACCCTTAGGGTTTCACGAGCTTAATCAATCAATT TAC
IR (inverted repeat)	[1-31] [33-63] nt (GTAAATTGATTGATTAAGCTCGTGAAACCCT..AGGGTTTCACGAGCTTAATCAATCAATTTAC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	ColE1 superfamily
	L:1-31;R:33-63

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTAAATTGATTGATTAAGCTCGTGAAACCCTTAGGGTTTCACGAGCTTAATCAATCAATT

61

TAC

Reference

[1] Giacomini A et al (2000) Nucleotide sequence and analysis of plasmid pMD136 from Pediococcus pentosaceus FBB61 (ATCC43200) involved in pediocin A production. Plasmid. 43(2):111-22. [PMID:10686129]

I. Information of Relaxase

ID	159
Name	MobA_pMD136
GenBank accession number	NP_037562
Family	MOB_P
Length	386 aa
UniProt ID	Q9WW30
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.50E-64, Aligned region: 8..248
Note	relaxase
Protein sequence [Download]	MATTHIKRSDSASRLVNYAEKRAVLKDGLNLDVDYAKSQLKQVREVYGNQGKTQAYASRI SFSPKELDPTNENDQQKALEIAKEVYQKTYPNQQVALYEHADTNALHVHAVIGAIDLETG RKMHGNWQEYREKLVHNTDEISQEHGLEVTQVDPNRYEKRTMAEIKMQAKGQPTWKDQIR QAVDKTMANRLVTDFQTFRDDLKQKTVDVWERGKDLTYQLTGTNYKARGNKLGTAYEKGE IYHELERRTRNHTKTRNPDTAKPDRTQPNLERNQELSGHANSITEQTTDRIDRTAPTDRA SQSTGQSGFSNDLEQFRKQQRDQQTTINRDAQRRARTVQRANTQDKSKDNRSLKTDKQRP KEQQRDVEKSQQQSKSNRSRNDGPSR

Reference

[1] Giacomini A et al (2000) Nucleotide sequence and analysis of plasmid pMD136 from Pediococcus pentosaceus FBB61 (ATCC43200) involved in pediocin A production. Plasmid. 43(2):111-22. [PMID:10686129]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	229	MobB_pMD136	NP_037563	196 aa	_
2	230	MobC_pMD136	NP_037561	73 aa	_

Information of T4CP

ID	159
Name	ORF1_pMD136
SecReT4 accesion number	_
GenBank accession number	NP_037556
Family	_
Length	607
UniProt ID	Q9XDL7
PDB ID	_
Pfam	AAA_15 [PF13175.5], Evalue: 1.30E-65, Aligned region: 1..373 AAA_21 [PF13304.5], Evalue: 4.10E-08, Aligned region: 155..376 AAA_23 [PF13476.5], Evalue: 8.50E-10, Aligned region: 5..282 DUF2813 [PF11398.7], Evalue: 1.20E-06, Aligned region: 1..70 AAA_29 [PF13555.5], Evalue: 3.30E-07, Aligned region: 3..58
Note	shows homology to conjugative coupling proteins
Protein sequence [Download]	MHLVSVTIHNFRGYKNTKINIDDQLTTLIGKNDVGKSTIVDALEIFFNNTVVKLDTNDLN IYATDSKLVSISCEFSDIPESIIIDSNQQTNLKDEYLVNANGNLEIKKVYDCSKKTPKPQ IFAVVHYPDNPEIADLLYLTHSKLLTALKKLPPAIQETVTSRVANAPIRQAIRSAYNITN FLTKDIDLQKVDGKTLWDKIETCLPIYALFQSDRPSNDSDAEVQDPMNTAIQESLSNAAQ QLQVIQQQVIQEVTAVAEMTISKLKEMDPEIAKGLEPQYQESPKWNKLFKFVIADEKGIP LNKRGSGVRRLVLLNFFRAKVSMTSDKLKTNGLIYAVEEPETAQHPDNQRKIMQALLELG QRGNCQIIITTHLAETAKMAPKSGVRLITRQDNVTKVSDNDTALKIAAKEVGLIANITDT NIVLYVEGPSDVLFFNSVSKILNQFDSTKYSDFTKSKNLLIVPMGGGTMKQWVNYKYLET LQLPSFYLFDKDLDAQHQREVDELKTDPQCLYAFLTDKREIENYIAPAAIERYFSKLLKS EFSMPELNSESDVTNLLKKAGVNQRQSYLKETLNSKVAAQMTADEFLSNDTTGFMAEFIA KITKEIS

Reference

[1] Francia MV et al (2004) Nucleotide sequence and analysis of plasmid pMD136 from Pediococcus pentosaceus FBB61 (ATCC43200) involved in pediocin A production. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]

Information of plasmid

ID	159
Plasmid name	pMD136
GenBank accession number	NC_001277.1
Genome size	19515 bp
Coordinate of oriT [Strand]	3761..3823 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Pediococcus pentosaceus ATCC43200 [1255]

Reference
N/A