 oriTDB accession number | 100164 |
| Name | oriT_pCD4  |
Sequence Completeness  | intact |
| oriT length | 464 nt |
| oriT sequence | TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGA AAAAGAGGGACGATTTCGGAAGAAGAAACTCGTCTCTTTTTTTCTTCTTTTTGTATGACA AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAA GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC AACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTAT GGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATACTTATAA CAAAATCAATGACAAAGGGCGATTGAGAAATGAGCGCTGGGGCATTTTATATTTGAGTAA GTTATTGATGGATCAGAAAAATGTATCACAAATTTAAACAAAG |
| IR (inverted repeat) | IR1: [139-148] [153-162] nt (CCCATTTTAT..ATAAAATGGG) IR2: [208-220] [224-236] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [243-256] [269-282] nt (ACATTGTAATACAA..TTGTATTACAATGT) |
| Location of nic site | [290-291] nt |
| Conserved sequence flanking the nic site | CTTG|CA |
| Note | similar ot oriT of pGL3, pGL4, pSRQ800, pSRQ900 and pIL7 |
Visualization of oriT structureTTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGA
AAAAGAGGGACGATTTCGGAAGAAGAAACTCGTCTCTTTTTTTCTTCTTTTTGTATGACA
AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAA
GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC
AACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTAT
GGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATACTTATAA
CAAAATCAATGACAAAGGGCGATTGAGAAATGAGCGCTGGGGCATTTTATATTTGAGTAA
GTTATTGATGGATCAGAAAAATGTATCACAAATTTAAACAAAG
Reference| [1] Emond E et al (2001) Molecular characterization of a theta replication plasmid and its use for development of a two-component food-grade cloning system for Lactococcus lactis. Appl Environ Microbiol. 67(4):1700-9. [PMID:11282624] |
| [2] Strahinic I et al (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703] |