 oriTDB accession number | 100163 |
| Name | oriT_pRAS3.2  |
Sequence Completeness  | core |
| oriT length | 47 nt |
| oriT sequence | CAGGATGCGGATTGTCATACACATGCTACGCATGTTCCTGCCAATCC |
| IR (inverted repeat) | [1-18] [22-39] nt (GGATTGGCAGGAACATGC..GCATGTGTATGACAATCC) |
| Location of nic site | [47-48] nt |
| Conserved sequence flanking the nic site | CATCCTG|T |
| Note | putative oriT nick region |
Visualization of oriT structure Reference| [1] L'Abée-Lund TM et al (2002) A global non-conjugative Tet C plasmid, pRAS3, from Aeromonas salmonicida. Plasmid. 47(3):172-81. [PMID:12151232] |
| [2] Loftie-Eaton W et al (2009) Comparative biology of two natural variants of the IncQ-2 family plasmids, pRAS3.1 and pRAS3.2. J Bacteriol. 191(20):6436-46. [PMID:19684126] |
| ID | 157 |
| Name | RepB/MobA_pRAS3.2 |
| GenBank accession number | NP_387464 |
| Family | MOBP |
| Length | 833 aa |
| UniProt ID | Q939A9 |
| PDB ID | _ |
| Pfam | RepB_primase [PF16793.4], Evalue: 1.40E-70, Aligned region: 491..750 Relaxase [PF03432.13], Evalue: 6.00E-30, Aligned region: 6..217 |
| Note | relaxase |
| Protein sequence [Download] | MIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELGWKDHQAIYGLHSDTD NIHLHIVINRVHPETLKIVEKNRGFDIELAHKAIARIEHAQGWQREQNGRYQVLENGELG RAPYDPEKPRQPDQKKRDMENRTGEKSAHRIAIEDGAAIIKQAQTWEQLHRELAAKGMRY EKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQRQQVAPREPEPIKPDVPG WKDYITGRKAHYAEKNADKLAQDKRQEQERKQLAEQQKARRDELMRGNWKGKGEVLNAMR SVIAAEQAAEKAALKEKHQKEREQHRQRFRPYPDLEQWQRMQKSPELAEQWRHRASEPQR IEGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEEAGRGGGVSFVDKGKSIDIHDWRNRDST LAALQLSAQKWGSFTVMGNDEYKAMCGKLAAEHGFKITNPELQESIQQERQRIQQERVQA MKSEQLKQFERYAEAVGAERYRVTSIKMREDGSKQTFILDKKDGITRGFTPQEIEQRTPE MQRLQRRGENLYYTPLSDKKHHILIDDMNREKLERLIRDGYQPAAVLESSPGNYQAIITV PKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHQREDGSYPEVRLL KAERRECIKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAYQRHYRD VLKRQRGGEVDLSRLDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRT ARYAYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSL |
Reference| [1] L'Abée-Lund TM et al (2002) A global non-conjugative Tet C plasmid, pRAS3, from Aeromonas salmonicida. Plasmid. 47(3):172-81. [PMID:12151232] |
| [2] Loftie-Eaton W et al (2009) Comparative biology of two natural variants of the IncQ-2 family plasmids, pRAS3.1 and pRAS3.2. J Bacteriol. 191(20):6436-46. [PMID:19684126] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 227 | MobC_pRAS3.2 | NP_387458 | 118 aa | _ |
| ID | 157 |
| Plasmid name | pRAS3.2 |
| GenBank accession number | NC_003124.1 |
| Incompatibility group | IncQ2 |
| Genome size | 11823 bp |
| Coordinate of oriT [Strand] | 1..47 [+] |
| Drug resistance | resistance to tetracycline |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Aeromonas salmonicida [645] |
Reference