oriTDB

The information of the oriT region

oriTDB accession number	100161
Name	oriT_pTX14-2
Sequence Completeness	incomplete
oriT length	24 nt
oriT sequence	TTTGGTATAGTAGGCTATACCAAA
IR (inverted repeat)	[1-9] [16-24] nt (TTTGGTATA..TATACCAAA)
Location of nic site	[14-15] nt
Conserved sequence flanking the nic site	TAGTAGG\|CTAT
Note	pMV158 superfamily
	L:1-9;R:16-24;D:14-15

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTTGGTATAGTAGGCTATACCAAA

Reference

[1] Andrup L et al (2003) The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid. 49(3):205-32. [PMID:12749835]

I. Information of Relaxase

ID	155
Name	Mob14-2_pTX14-2
GenBank accession number	NP_705748
Family	MOB_V
Length	445 aa
UniProt ID	Q8GLD7
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 4.60E-71, Aligned region: 1..192
Note	relaxase
Protein sequence [Download]	MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNYDLLNQQKIRYESTIK QEISERVKRKPRANSVVLSEFVVTASPDYMHSLSLEEQKRYFESSLAFIQKRYGKQNTLY AMVHMDEATPHMHIGVMPITEDNRLSAKDMFMRKELISLQQDFPLEMREKGFDVERGEGS EKKHLSPQAFKEKQDLEVEVEQLSNVKTHLKTKVVETHNQLQQTTNYIEKQNETLQKIQQ QFLSLDKKIKEKKQEFETFRNQIPDKPVSMSYLREETKTEVTTKLFGKPEITEKKTGNIV VTREQWRDMTEKVNAAVIVKKDYERLQKTDLVKENQSLREDNKYLEETIEGNNLALKHSY KQNRKLEEVNKELHTEIGTLKAHIRDLQMNIKVLYQQTKKVFKEQFKAFRGLIKNELDMK GVDNQFEREHTREIRSRQKGYDMER

Reference

[1] Andrup L et al (2003) The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid. 49(3):205-32. [PMID:12749835]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	225	_

N/A

Information of plasmid

ID	155
Plasmid name	pTX14-2
GenBank accession number	NC_004334.1
Genome size	6829 bp
Coordinate of oriT [Strand]	2104..2127 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bacillus thuringiensis 4Q2 [1428]

Reference
N/A