oriTDB
The information of the oriT region
oriTDB accession number 100161
Name oriT_pTX14-2  insolico
Sequence Completeness incomplete
oriT length 24 nt
oriT sequence TTTGGTATAGTAGGCTATACCAAA
IR (inverted repeat)[1-9] [16-24] nt  (TTTGGTATA..TATACCAAA)
Location of nic site [14-15] nt
Conserved sequence flanking the
  nic site
TAGTAGG|CTAT
Note pMV158 superfamily
L:1-9;R:16-24;D:14-15
Visualization of oriT structure
Reference
[1] Andrup L et al (2003) The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid. 49(3):205-32. [PMID:12749835]
I. Information of Relaxase
ID 155
Name Mob14-2_pTX14-2 insolico
GenBank accession number NP_705748
Family MOBV
Length 445 aa
UniProt ID Q8GLD7
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 4.60E-71, Aligned region: 1..192
Note relaxase
Protein sequence [Download] MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNYDLLNQQKIRYESTIK
QEISERVKRKPRANSVVLSEFVVTASPDYMHSLSLEEQKRYFESSLAFIQKRYGKQNTLY
AMVHMDEATPHMHIGVMPITEDNRLSAKDMFMRKELISLQQDFPLEMREKGFDVERGEGS
EKKHLSPQAFKEKQDLEVEVEQLSNVKTHLKTKVVETHNQLQQTTNYIEKQNETLQKIQQ
QFLSLDKKIKEKKQEFETFRNQIPDKPVSMSYLREETKTEVTTKLFGKPEITEKKTGNIV
VTREQWRDMTEKVNAAVIVKKDYERLQKTDLVKENQSLREDNKYLEETIEGNNLALKHSY
KQNRKLEEVNKELHTEIGTLKAHIRDLQMNIKVLYQQTKKVFKEQFKAFRGLIKNELDMK
GVDNQFEREHTREIRSRQKGYDMER
Reference
[1] Andrup L et al (2003) The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid. 49(3):205-32. [PMID:12749835]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 225 _ insolico
 N/A
Information of plasmid
ID 155
Plasmid name pTX14-2
GenBank accession number NC_004334.1
Genome size 6829 bp
Coordinate of oriT  [Strand] 2104..2127 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bacillus thuringiensis 4Q2 [1428]
Reference
 N/A