oriTDB

The information of the oriT region

oriTDB accession number	100160
Name	oriT_pGI1
Sequence Completeness	incomplete
oriT length	26 nt
oriT sequence	GTAAACTGTAGTGTGCTACAGTTTAC
IR (inverted repeat)	[1-10] [17-26] nt (GTAAACTGTA..TACAGTTTAC)
Location of nic site	[15-16] nt
Conserved sequence flanking the nic site	TAGTGTG\|CTA
Note	pMV158 superfamily
	L:1-10;R:17-26;D:15-16

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTAAACTGTAGTGTGCTACAGTTTAC

Reference

[1] Andrup L et al (2003) The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid. 49(3):205-32. [PMID:12749835]

I. Information of Relaxase

ID	154
Name	Mob1_pGI1
GenBank accession number	NP_705753
Family	MOB_V
Length	401 aa
UniProt ID	Q8GLD2
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 4.00E-64, Aligned region: 1..196
Note	relaxase
Protein sequence [Download]	MSKFMVCHMQKFKMTDVKGLQIHNQREKESHSNSDIIQERTEQNYDLIHDKRKVDYKKII QNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNFLSPVEQRRFFEESVCFLKARYGEEN FVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDLQDKIYEHVKEKGFDIER GVSSDRKHLSTQRFKAVTLQQEIEKLEQEKKEIDSRLYDLKFSLNQAKSVDEIPVKEKGG FIRSKTVEIDSEDFESIKVLAKSSEVLRSENRRLKNEKIKIEREKDDLYKGQRFLERQVT DLKRENRGLKEANDFLKKTLERVKEMYKEKLPELAGMIGYVKGSILDKMNRKFLKRHFAG DDEVRGAQKFLNHKQEHEEQQKRLKQVRRSQQKNRDQGLER

Reference

[1] Andrup L et al (2003) The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid. 49(3):205-32. [PMID:12749835]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	224	_

N/A

Information of plasmid

ID	154
Plasmid name	pGI1
GenBank accession number	NC_004335.1
Genome size	8254 bp
Coordinate of oriT [Strand]	6421..6446 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bacillus thuringiensis H1.1 [1432]

Reference
N/A