oriTDB
The information of the oriT region
oriTDB accession number 100160
Name oriT_pGI1  insolico
Sequence Completeness incomplete
oriT length 26 nt
oriT sequence GTAAACTGTAGTGTGCTACAGTTTAC
IR (inverted repeat)[1-10] [17-26] nt  (GTAAACTGTA..TACAGTTTAC)
Location of nic site [15-16] nt
Conserved sequence flanking the
  nic site
TAGTGTG|CTA
Note pMV158 superfamily
L:1-10;R:17-26;D:15-16
Visualization of oriT structure
Reference
[1] Andrup L et al (2003) The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid. 49(3):205-32. [PMID:12749835]
I. Information of Relaxase
ID 154
Name Mob1_pGI1 insolico
GenBank accession number NP_705753
Family MOBV
Length 401 aa
UniProt ID Q8GLD2
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 4.00E-64, Aligned region: 1..196
Note relaxase
Protein sequence [Download] MSKFMVCHMQKFKMTDVKGLQIHNQREKESHSNSDIIQERTEQNYDLIHDKRKVDYKKII
QNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNFLSPVEQRRFFEESVCFLKARYGEEN
FVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDLQDKIYEHVKEKGFDIER
GVSSDRKHLSTQRFKAVTLQQEIEKLEQEKKEIDSRLYDLKFSLNQAKSVDEIPVKEKGG
FIRSKTVEIDSEDFESIKVLAKSSEVLRSENRRLKNEKIKIEREKDDLYKGQRFLERQVT
DLKRENRGLKEANDFLKKTLERVKEMYKEKLPELAGMIGYVKGSILDKMNRKFLKRHFAG
DDEVRGAQKFLNHKQEHEEQQKRLKQVRRSQQKNRDQGLER
Reference
[1] Andrup L et al (2003) The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid. 49(3):205-32. [PMID:12749835]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 224 _ insolico
 N/A
Information of plasmid
ID 154
Plasmid name pGI1
GenBank accession number NC_004335.1
Genome size 8254 bp
Coordinate of oriT  [Strand] 6421..6446 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bacillus thuringiensis H1.1 [1432]
Reference
 N/A