oriTDB

The information of the oriT region

oriTDB accession number	100159
Name	oriT_pKJK5
Sequence Completeness	intact
oriT length	96 nt
oriT sequence	GGGACAGTGAAGATAGATAACCGGCTTGCCGGTTAGCTAACTTCACCCATCCTGCCCGCC TTACGGCGACGTTAACGCCAAGAATGAAATCAGATT
IR (inverted repeat)	[7-25] [28-46] nt (GTGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCAC)
Location of nic site	[54-55] nt
Conserved sequence flanking the nic site	CATCCTG\|C
Note	_
	L:7-25;R:28-46;D:54-55

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGACAGTGAAGATAGATAACCGGCTTGCCGGTTAGCTAACTTCACCCATCCTGCCCGCC

61

TTACGGCGACGTTAACGCCAAGAATGAAATCAGATT

Reference

[1] Pachulec E et al (2010) Conjugative Plasmids of Neisseria gonorrhoeae. PLoS One. 5(4):e9962. [PMID:20376355]

[2] Bahl MI et al (2007) The multiple antibiotic resistance IncP-1 plasmid pKJK5 isolated from a soil environment is phylogenetically divergent from members of the previously established alpha, beta and delta sub-groups. Plasmid. 58(1):31-43. [PMID:17306874]

I. Information of Relaxase

ID	153
Name	TraI_pKJK5
GenBank accession number	CAK02686
Family	MOB_P
Length	740 aa
UniProt ID	H2EQ20
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.20E-57, Aligned region: 12..252
Note	relaxase
Protein sequence [Download]	MIAKHVPMRSLGKSDFAGLVQYITDAQSKTERLGLVTLTNCQAGTVQAATDEVLATQHMN TRATGDKTFHLLVSFRAGEKPDADTLKAIEERICAGLGYGEHQRISAVHHDTDNLHIHIA INKIHPTRNTMHEPFQSYRTLGELCEVLERDYGLEKDNHTPARSIAEGRAADMERHAGVE SLVGWIKRECLEEIKAAQSWTELHQALQANGLELRAKGNGFVIEAGDGTQVKASTVAREL SKPKLEARFGPFEASPERQAQTKAKRQYQKKPVRLRVNTTELYARYKADQQTLAAAQRAA LDKARQSKTRKVEDAKRSSRLRRAAIKLMGGKAATKKLLYAQASKALRDEIQAINKEHQR ERQGCYDEHKRRTWADWLKKEALQGNAEALAALRAREAAQGLKGNTVEGQGQAKPGHAPV VDNITKKGTIIFRAGKTAVRDDGDKLQVSREATREGLQQALRLAMERYGSRITVNGTTEF KAQIIRAAVDSQLPITFADPALESRRQALLKKENTHDRPQQDRGRTGRGTGRPGSRPAAD NDAARSSVNADRGGRDPAGRVHATGQHRKPDIGRIGRVPPPQSKNRLRALSKLGVVRIAS GSEVLLPRDVPGHMEQQGTQPDNALRRGVSRPGGRLEAGPQYKPEQVKAMDKYIAEREGK RLKGFDIPKHSRYTAGDGALSFAGMRNVEGQTLALVKRGDDVLVMPIDQATANRLKRIAV GDAVSITPKGSIKTSKGRSR

Reference

[1] Bahl MI et al (2007) The multiple antibiotic resistance IncP-1 plasmid pKJK5 isolated from a soil environment is phylogenetically divergent from members of the previously established alpha, beta and delta sub-groups. Plasmid. 58(1):31-43. [PMID:17306874]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	221	TraH_pKJK5	CAK02687	120 aa	involved in conjugative DNA-transfer
2	222	TraJ_pKJK5	CAK02688	124 aa	conjugal transfer relaxosome component TraJ; oriT binding protein
3	223	TraK_pKJK5	CAK02689	129 aa	_

Information of T4CP

ID	153
Name	TraG_pKJK5
SecReT4 accesion number	111038096
GenBank accession number	CAK02685
Family	VirD4/TraG
Length	634
UniProt ID	H2EQ83
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 1.10E-161, Aligned region: 121..624 TraG-D_C [PF12696.6], Evalue: 1.80E-31, Aligned region: 444..569 TrwB_AAD_bind [PF10412.8], Evalue: 1.40E-23, Aligned region: 177..584
Note	conjugal transfer coupling protein TraG; Provisional
Protein sequence [Download]	MKIKMNNAVGPQVRSAKPKAGKLLPTLGVLSLAGGLQAATQYFAHTFDYQASLGGHIGHI YAPWSILQWASKWYSHYPNEIMSAGSVGMLVSTVGLLGVAIAKVVASNTSKANQFLHGSA RWAEKKDIQAAGLLPRPRTVLEVVTGKEEPTATGVYVGGWEDKDGSFYYLRHSGPEHVLC YAPTRSGKGVGLVVPTMLSWPASAVITDLKGELWALTAGWRKKHAKNKVLRFEPASSNGG ICWNPLDEIRLGTEFEVGDVQNLAQLIVDPDGKGLDSHWQKTAFALLVGVILHALYKAQA DGTYATLPSVDAMLADPNRDVGELWMEMATYGHVDGQNHHAIGSAARDMMDRPEEEAGSV LSTAKSYLSLYRDPVVARSVSKSEFRIKDLMHQDDPASLYIVTQPNDKARLRPLVRIMVN MIVRLLADKMDFENGRPVAHYKHRLLMMLDEFPSLGKLEILQESLAFVAGYGIKCYLICQ DINQLKSRETGYGHDETITSNCHVQNAYPPNRVETAEHLSRLTGQTTIVKEQITTSGRRT AAMLGQVSRTFQEVQRPLLTPDECLRMPGPRKSATGEIEEAGDMVVYVAGYPAIYGKQPL YFKDPVFQARAAIPAPKVSDKTIHVQDAGQGITI

Reference

[1] Bahl MI et al (2007) The multiple antibiotic resistance IncP-1 plasmid pKJK5 isolated from a soil environment is phylogenetically divergent from members of the previously established alpha, beta and delta sub-groups. Plasmid. 58(1):31-43. [PMID:17306874]

Information of plasmid

ID	153
Plasmid name	pKJK5
GenBank accession number	AM261282.1
Incompatibility group	IncP1
Genome size	54383 bp
Coordinate of oriT [Strand]	44857..44952 [+]
Drug resistance	resistance to spectinomycin, tetracycline and trimethoprim
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Uncultured bacterium [77133]

Reference

[1] Oliveira CS et al (2013) Comparative genomics of IncP-1ε plasmids from water environments reveals diverse and unique accessory genetic elements. Plasmid. 70(3):412-9. [PMID:23831558]

[2] Bahl MI et al (2007) The multiple antibiotic resistance IncP-1 plasmid pKJK5 isolated from a soil environment is phylogenetically divergent from members of the previously established alpha, beta and delta sub-groups. Plasmid. 58(1):31-43. [PMID:17306874]