oriTDB

The information of the oriT region

oriTDB accession number	100158
Name	oriT_pSMQ172
Sequence Completeness	core
oriT length	42 nt
oriT sequence	GCACATTTACGAAGTAAAGTATAGTGCGTTATACTTTACATG
IR (inverted repeat)	[3-10] [14-20] nt (ACATTTAC..GTAAAGT)
Location of nic site	[28-29] nt
Conserved sequence flanking the nic site	TAGTGCG\|TTAT
Note	pMV158 superfamily; the conserved recombination site (RS_A): AAGTATAGTGCG\|TTATACT
	L:3-10;R:14-20;D:28-29

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GCACATTTACGAAGTAAAGTATAGTGCGTTATACTTTACATG

Reference

[1] Turgeon N et al (2001) Isolation and characterization of a Streptococcus thermophilus plasmid closely related to the pMV158 family. Plasmid. 45(3):171-83. [PMID:11407913]

I. Information of Relaxase

ID	152
Name	Mob_pSMQ172
GenBank accession number	AAK83121
Family	MOB_V
Length	499 aa
UniProt ID	Q93PW6
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 3.30E-73, Aligned region: 1..194
Note	relaxase
Protein sequence [Download]	MSYMVARMQKMKAGNLGGAYKHNERVFETHSNKDIDPSRSPLNYELTDRDHSVSYEKQIK DYVNENKISNRAIRKDAVLCDEWIITSDKSFFEKLSEGETREFFETAKNYFAENYGEDNI AYASVHLDESTPHMHMGVVPFQDGKLSSKAMFDKEELKKIQEDLPKYMNEHGFELERGEL NSEAKHKTVAEFKQEMAGKEIEKQLVLEYGAPEYVNHQEEFVTKEEYQEAYEVFQNELGA WVDKSDFSWRETTFQEKIDWVKNHQQEELQQLVSSRKPLEDEIRTLNEVLKEKYEELDKI ELRASESLSELSGVEGYINNLEKHSNALESKIGGLENEYLRLAKQNARLSDLKIMSEKEL AEIQPKKGMFGKEHVELTKEQFEKFKGLIYRSKNLVHEKELENDQLRRQMPLRSSKNGFE ASLQWVKDKTKGESIDRLKSEIRALKNENSVLRQQNDKMLGKLREFMPDKALKNFVSELK AIQPIVKIVKRVIEKGLGL

Reference

[1] Turgeon N et al (2001) Isolation and characterization of a Streptococcus thermophilus plasmid closely related to the pMV158 family. Plasmid. 45(3):171-83. [PMID:11407913]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	220	_

N/A

Information of plasmid

ID	152
Plasmid name	pSMQ172
GenBank accession number	AF295100.1
Genome size	4230 bp
Coordinate of oriT [Strand]	1332..1373 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Streptococcus thermophilus SMQ-172 [1308]

Reference
N/A