oriTDB
The information of the oriT region
oriTDB accession number 100158
Name oriT_pSMQ172  insolico
Sequence Completeness core
oriT length 42 nt
oriT sequence GCACATTTACGAAGTAAAGTATAGTGCGTTATACTTTACATG
IR (inverted repeat)[3-10] [14-20] nt  (ACATTTAC..GTAAAGT)
Location of nic site [28-29] nt
Conserved sequence flanking the
  nic site 		TAGTGCG|TTAT
Note pMV158 superfamily; the conserved recombination site (RSA): AAGTATAGTGCG|TTATACT
L:3-10;R:14-20;D:28-29
Visualization of oriT structure
Reference
[1] Turgeon N et al (2001) Isolation and characterization of a Streptococcus thermophilus plasmid closely related to the pMV158 family. Plasmid. 45(3):171-83. [PMID:11407913]
I. Information of Relaxase
ID 152
Name Mob_pSMQ172 insolico
GenBank accession number AAK83121
Family MOBV
Length 499 aa
UniProt ID Q93PW6
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 3.30E-73, Aligned region: 1..194
Note relaxase
Protein sequence [Download] MSYMVARMQKMKAGNLGGAYKHNERVFETHSNKDIDPSRSPLNYELTDRDHSVSYEKQIK
DYVNENKISNRAIRKDAVLCDEWIITSDKSFFEKLSEGETREFFETAKNYFAENYGEDNI
AYASVHLDESTPHMHMGVVPFQDGKLSSKAMFDKEELKKIQEDLPKYMNEHGFELERGEL
NSEAKHKTVAEFKQEMAGKEIEKQLVLEYGAPEYVNHQEEFVTKEEYQEAYEVFQNELGA
WVDKSDFSWRETTFQEKIDWVKNHQQEELQQLVSSRKPLEDEIRTLNEVLKEKYEELDKI
ELRASESLSELSGVEGYINNLEKHSNALESKIGGLENEYLRLAKQNARLSDLKIMSEKEL
AEIQPKKGMFGKEHVELTKEQFEKFKGLIYRSKNLVHEKELENDQLRRQMPLRSSKNGFE
ASLQWVKDKTKGESIDRLKSEIRALKNENSVLRQQNDKMLGKLREFMPDKALKNFVSELK
AIQPIVKIVKRVIEKGLGL
Reference
[1] Turgeon N et al (2001) Isolation and characterization of a Streptococcus thermophilus plasmid closely related to the pMV158 family. Plasmid. 45(3):171-83. [PMID:11407913]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 220 _ insolico
 N/A
Information of plasmid
ID 152
Plasmid name pSMQ172
GenBank accession number AF295100.1
Genome size 4230 bp
Coordinate of oriT  [Strand] 1332..1373 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Streptococcus thermophilus SMQ-172 [1308]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU