oriTDB
The information of the oriT region
oriTDB accession number 100156
Name oriT_pSRQ800  insolico
Sequence Completeness intact
oriT length 415 nt
oriT sequence CATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTC TTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGGAGGTG GCGTTTGCCTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTC CCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTACTTTGTATTACAATGTGATAGC TTGCAGTATTTATGGTTTTATAGTTGCTATTTTGTTATGAGGATTGTAACCGAATAGGGC GCAATACTTATTACAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTT ATCTTTGAGTAAGTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG
IR (inverted repeat)IR1: [91-100] [105-114] nt  (CCCATTTTAT..ATAAAATGGG)
IR2: [160-172] [176-188] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [195-208] [221-234] nt  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site [243-244] nt
Conserved sequence flanking the
  nic site
CTTG|CA
Note similar to lactococcal plasmids transfer origins (pGL3, pGL4, pCD4, pSRQ900 and pIL7)
L:91-100;R:105-114;L:160-172;R:176-188;L:195-208;R:221-234;D:243-244
Visualization of oriT structure
Reference
[1] Boucher I et al (2001) DNA sequence analysis of three Lactococcus lactis plasmids encoding phage resistance mechanisms. J Dairy Sci. 84(7):1610-20. [PMID:11467810]
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Information of plasmid
ID 150
Plasmid name pSRQ800
GenBank accession number NC_004960.1
Genome size 7858 bp
Coordinate of oriT  [Strand] 6826..7240 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis W-1 [1358]
Reference
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