oriTDB

The information of the oriT region

oriTDB accession number	100154
Name	oriT_pUO1
Sequence Completeness	intact
oriT length	373 nt
oriT sequence	GCCTCGCCTCGTCTCTCATGCCGGCGGTAGCCGGCTGCCTCGCAGAGCAGGATGACCCGT TGAGCGCCCCCGGCGCGAATAAGGGACAGTGAAGATAGATAACCGGCTCGCCGGTTAGCT AACTTCACACATCCTGCCCGCCTTACGGCGTTAATAACACCAAGGAAAGTCTACACCAGC CATTACGATTTATCCGCAACTATCGCGCTATCAGGCCGCAAAAGCAGCAACGGATATAGC GAAAACCGCCACAATGGCCCATAATGCCGCTATCGAAGCGTGCCAATGCACGCCGATAGC GGACTTTTTGCGTTTCCGTAGCGCCGCTTAGTAGCGTTACATTTGCGATGAGAGGATTAG ATGGACGAACACG
IR (inverted repeat)	IR1: [64-68] [72-76] nt (GCGCC..GGCGC) IR2: [89-107] [110-128] nt (GTGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCAC)
Location of nic site	[136-137] nt
Conserved sequence flanking the nic site	CATCCTG\|C
Note	_
	L:64-68;R:72-76;L:89-107;R:110-128;D:136-137

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GCCTCGCCTCGTCTCTCATGCCGGCGGTAGCCGGCTGCCTCGCAGAGCAGGATGACCCGT

61

TGAGCGCCCCCGGCGCGAATAAGGGACAGTGAAGATAGATAACCGGCTCGCCGGTTAGCT

121

AACTTCACACATCCTGCCCGCCTTACGGCGTTAATAACACCAAGGAAAGTCTACACCAGC

181

CATTACGATTTATCCGCAACTATCGCGCTATCAGGCCGCAAAAGCAGCAACGGATATAGC

241

GAAAACCGCCACAATGGCCCATAATGCCGCTATCGAAGCGTGCCAATGCACGCCGATAGC

301

GGACTTTTTGCGTTTCCGTAGCGCCGCTTAGTAGCGTTACATTTGCGATGAGAGGATTAG

361

ATGGACGAACACG

Reference

[1] Sota M et al (2003) Structure of haloacetate-catabolic IncP-1beta plasmid pUO1 and genetic mobility of its residing haloacetate-catabolic transposon. J Bacteriol. 185(22):6741-5. [PMID:14594853]

I. Information of Relaxase

ID	148
Name	TraI_pUO1
GenBank accession number	NP_904299
Family	MOB_P
Length	746 aa
UniProt ID	Q76M03
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 9.90E-64, Aligned region: 12..252
Note	conjugal transfer relaxase TraI
Protein sequence [Download]	MIAKHVPMRSLGKSDFAGLANYITDAQSKDHRLGHVQATNCEAGSIQDAITEVLATQHTN TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICVGLGYGEHQRISAVHNDTDNLHIHIA INKIHPTRHTMHEPYYPHRALAELCTALERDYGLERDNHEPRKRGAEGRAADMERHAGVE SLVGWIKRECLDEIKGAQSWQELHQVMRDNGMELRVRANGLVFEAGDGTMVKASTVARDL SKPSLEARLGPFEASPERQAQTTAKRQYRKDPIRLRVNTVELYAKYKAEQQSLTTARAQA LERARHRKDRLIEAAKRSNRLRRATIKVVGEGRANKKLLYAQASKALRSELQAINKQFQQ ERTALYAEHSRRTWADWLKKEAQHGGADALAALRAREAAQGLKGNTIRGEGQAKPGHAPA VDNITKKGTIIFRAGMSAVRDDGDRLQVSREATREGLQEALRLAMQRYGNRITVNGTVEF KAQMIRAAVDSQLPITFTDPALESRRQALLNKENTHERTERPEHRGRTGRGAGGPGQRPA ADQHATGAAAVARAGDGRPAAGRGDRADAGLHAATVHRKPDVGRLGRKPPPQSQHRLRAL SELGVVRITGGSEVLLPRDVPRHVEQQGTQPDHALRRGISRPGTGVGQTPPGVAAADKYI AEREAKRLKGFDIPKHSRYTAGDGALTFQGTRTIEGQALALLKRGDEVMVMPIDQATARR LTRIAVGDAVSITAKGSIKTSKGRSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	214	TraH_pUO1	NP_904300	130 aa	_
2	215	TraJ_pUO1	NP_904301	124 aa	conjugal transfer relaxosome component TraJ
3	216	TraK_pUO1	NP_904302	132 aa	_

Information of T4CP

ID	148
Name	TraG_pUO1
SecReT4 accesion number	34500527
GenBank accession number	NP_904298
Family	VirD4/TraG
Length	637
UniProt ID	Q76M04
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 2.70E-162, Aligned region: 121..624 TraG-D_C [PF12696.6], Evalue: 5.50E-32, Aligned region: 444..569 TrwB_AAD_bind [PF10412.8], Evalue: 5.10E-22, Aligned region: 187..586
Note	conjugal transfer coupling protein TraG
Protein sequence [Download]	MKIKMNNAVGPQVRTAKPKPSKLLPVLGAASMVGGLQAATQFFAHTFAYHATLGPNVGHV YAPWSILHWTYKWYSQYPDEIMKAGSMGMLVSTVGLLGVAVAKVVTSNSSKANEYLHGSA RWAEKKDIQAAGLLPRERNVLEIVTGKAAPTATGVYVGGWQDKDGNFFYLRHSGPEHVLT YAPTRSGKGVGLVVPTLLSWGASSVITDLKGELWALTAGWRQKHAKNKVLRFEPASTSGG VCWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLDSHWQKTAFALLVGVILHALYKAKD DGGTATLPSVDAMLADPNRDIGELWMEMATYGHVDGQNHHAIGSAARDMMDRPEEEAGSV LSTAKSYLALYRDPVVARNVSRSDFRIKQLMHEDDPVSLYIVTQPNDKARLRPLVRVMVN MIVRLLADKMDFEGGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQ DINQLKSRETGYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRT AAMLGQVSRTYQEVQRPLLTPDECLRMPGPKKNAQGEIEEAGDMVIYVAGYPAIYGKQPL YFKDPVFSARAAIPAPKVSDRLRAVAQAETEGEGITI

Reference
N/A

Information of plasmid

ID	148
Plasmid name	pUO1
GenBank accession number	NC_005088.1
Incompatibility group	IncP1
Genome size	67066 bp
Coordinate of oriT [Strand]	56496..56868 [+]
Drug resistance	_
Heavy-metal resistance	mercury resistance
Virulence factor	_
Xenobiotic degradation	haloacetate-catabolic
Host bacterium [NCBI Taxonomy ID]	Delftia acidovorans B [80866]

Reference

[1] Sota M et al (2003) Structure of haloacetate-catabolic IncP-1beta plasmid pUO1 and genetic mobility of its residing haloacetate-catabolic transposon. J Bacteriol. 185(22):6741-5. [PMID:14594853]