 oriTDB accession number | 100152 |
| Name | oriT_pPMA4326A  |
Sequence Completeness  | intact |
| oriT length | 86 nt |
| oriT sequence | GTTCCAAAGGGGTATCCCCTTTGGCACGTCGATTGGCTACGAAAAAGGCGAAGCTCTTTT TTGTAGACAATCGGTAACGTGCCTGT |
| IR (inverted repeat) | IR1: [1-12] [16-27] nt (GTTCCAAAGGGG..CCCCTTTGGCAC) IR2: [30-47] [56-73] nt (CGATTGGCTACGAAAAAG..CTTTTTTGTAGACAATCG) |
| Location of nic site | [81-82]nt |
| Conserved sequence flanking the nic site | TAACGTG|C |
| Note | _ |
Visualization of oriT structure Reference| [1] Stavrinides J et al (2004) Nucleotide sequence and evolution of the five-plasmid complement of the phytopathogen Pseudomonas syringae pv. maculicola ES4326. J Bacteriol. 186(15):5101-15. [PMID:15262947] |
| ID | 146 |
| Name | A47_pPMA4326A |
| GenBank accession number | YP_025665 |
| Family | MOBP |
| Length | 1245 aa |
| UniProt ID | Q6J2F7 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 1.80E-07, Aligned region: 9..267 |
| Note | relaxase |
| Protein sequence [Download] | MLIRVSGYNTGAQEYLEKGNKSGREFTRDELDHRLIIEGQLSLTRAIYESIPDYGQDRYL TFTLSFKEDTVSPELLKSITTDFKNFFMHAYKPEEFNLYAEAHLPKMKTVTDRKTGEVID RKPHIHIIIPRINLLSGNEANPVDVYKNHEKYFEAIQEHINQKYGLSSPRENVRADITDA ASVLSRYKGDDFYGKNRQFKQELVKQVIERGVTSRADFYALVAEHGETRIRNEGKDTEYI SVKLPGDAKGTNLKDTIFQDDFIVRRELKKPPLEASVIQERLLAWPQRAREIKYVNKATP KFRKTYSEASPEDRVRLLADREAKFYQVHGEHNDNVHTGQRQRDHQRSPAETAGRRTAAP ADGVQDLSVSDVADHRQAGSARSRDGALLLPSDAHVHLGQSQPGGDSGLRPPVPGGGRGR RNGSTAERGRGGSGPAAVSQEATGTATPAGATGRRRAGAGKPRNARVRAGRIVPPYAKNP HRVATIADIEERGRRLFDPLKKPSDNALVFYRTPSTPSNTPATATTTSGRSSAGRKPRTS GKPRPPRQWRPGSVVPPYAKNPHRVATVADIEQRARMLFDPLKRPADKALVFKRASIKAL TVNRQASTVAAYFTREAQHNRIAPAHRQAIRRIDQQYFALRRAVFSDQRLTRQDKAQLVS VLTFERLKAREQFHKPQPNTEVNFMGSAAIRNLLEDEKEDPGFSISGARGPGPEGVRDRV KRVMDRFAKQVDPAAASQRARDLSAKDLYTRKAKFSQNVHYLDKQTDKTLFVDTGKTISM RRTGITESGVSVALQLARERFGSTLTITGTADFKKLVIEAVAKNGLDVHFTDKAMNHSLA ARRAELDIERGGQSIGPATDLPRHVDDSTRDVRDQADRLAVTVPIEALYGEGKTAEQVSQ ALAAQLDTVPEPERIAFVELVAITLGIPERGEPKGDQAFAQWQAQRAQPAADVAATATSE AQANVPTPSDAAPEPVSPEAAKPARVNDPDLQSPSELVRREAQWRRDFPMSEADVRASDT VMGLRGEDHAIWIIATDDKTPEAAAMLTAYMENDSYREAFKSSLETAYRQVENWPQAVQD LDQITEMAVKIVQQVEARLYPAPQAASGQATPSRNKVIEGELLDHGPAPYQHKDDKQMSY FVTLKPEGGKPRTVWGVGLEDAMKDSNLKQGDQVRLEDLGTQPVVVQVIEEDGTVTDKTV NRREWSAQPTAPEREVAETTPKGQAAAAGTPELSSPDEEDGMSVD |
Reference| [1] Stavrinides J et al (2004) Nucleotide sequence and evolution of the five-plasmid complement of the phytopathogen Pseudomonas syringae pv. maculicola ES4326. J Bacteriol. 186(15):5101-15. [PMID:15262947] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 212 | A46_pPMA4326A | YP_025664 | 241 aa | relaxosome component |
| ID | 146 |
| Name | VirD4_pPMA4326A |
| SecReT4 accesion number | _ |
| GenBank accession number | YP_025656 |
| Family | VirD4/TraG |
| Length | 557 |
| UniProt ID | Q6J2G6 |
| PDB ID | _ |
| Pfam | T4SS-DNA_transf [PF02534.13], Evalue: 4.90E-72, Aligned region: 4..482 TraG-D_C [PF12696.6], Evalue: 5.00E-22, Aligned region: 313..439 TrwB_AAD_bind [PF10412.8], Evalue: 7.40E-12, Aligned region: 271..459 |
| Note | similar to Pseudomonas syringae pv. syringae A2 plasmid pPSR1 VirD4 encoded by GenBank Accession Number AY342395 |
| Protein sequence [Download] | MGKAKQQPISPDNPQERIEEHPAILIGKHPTKDTFLASYGQTFVMLAAPPGTGKTVGVVT PNLLSYPDSVVVNDPKFENWRDTAGFRAAAGHKVYRFSPELLETHRWNPLSALSRDPLYR LGQIRTLAGVLFVSDNPKNQEWYNKAANVFAAILLYLMEMEGMKLNGMKLTLPQAYEVAS LGTGLGVWAQQAIEQHSTGPNALSVETLRELNGVFEASKNKSSGWSTTVDILRGALSMYA EKTVAWAVSDTDIDFTKLRKEKISIYFCVTGNAIKKYGPLMNLFFTQAIQQNTDVMPEQG GHCADGTLILKYQVLFVIDEIAVMGRIEIMEMAPALTRGAGLRYIIIFQGKDQLRSERTY GREAGNGIMQAFHIEIVYGTGNVELAKEYSTRLGNTTVRVHNQSLNRQKHEIGARGQTDS YSEQPRPLLLPQEVSALPYGEELIFVEATKTTPAANIRARKIFWYEEPVFKERAGMPTPD VPIGDASKIDALTVPVRTVEAKVAVADTKPMQAEQRQRWNPKDKASEVAQAEAAKAQPPE VEPDPEPAQADDTPETM |
Reference| [1] Stavrinides J et al (2004) Nucleotide sequence and evolution of the five-plasmid complement of the phytopathogen Pseudomonas syringae pv. maculicola ES4326. J Bacteriol. 186(15):5101-15. [PMID:15262947] |
| ID | 146 |
| Plasmid name | pPMA4326A |
| GenBank accession number | NC_005918.1 |
| Incompatibility group | IncI |
| Genome size | 46697 bp |
| Coordinate of oriT [Strand] | 39285..39370 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Pseudomonas syringae pv. maculicola strain ES4326 [59511] |
Reference