oriTDB

The information of the oriT region

oriTDB accession number	100152
Name	oriT_pPMA4326A
Sequence Completeness	intact
oriT length	86 nt
oriT sequence	GTTCCAAAGGGGTATCCCCTTTGGCACGTCGATTGGCTACGAAAAAGGCGAAGCTCTTTT TTGTAGACAATCGGTAACGTGCCTGT
IR (inverted repeat)	IR1: [1-12] [16-27] nt (GTTCCAAAGGGG..CCCCTTTGGCAC) IR2: [30-47] [56-73] nt (CGATTGGCTACGAAAAAG..CTTTTTTGTAGACAATCG)
Location of nic site	[81-82]nt
Conserved sequence flanking the nic site	TAACGTG\|C
Note	_
	L:1-12;R:16-27;L:30-47;R:56-73;D:81-82

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTTCCAAAGGGGTATCCCCTTTGGCACGTCGATTGGCTACGAAAAAGGCGAAGCTCTTTT

61

TTGTAGACAATCGGTAACGTGCCTGT

Reference

[1] Stavrinides J et al (2004) Nucleotide sequence and evolution of the five-plasmid complement of the phytopathogen Pseudomonas syringae pv. maculicola ES4326. J Bacteriol. 186(15):5101-15. [PMID:15262947]

I. Information of Relaxase

ID	146
Name	A47_pPMA4326A
GenBank accession number	YP_025665
Family	MOB_P
Length	1245 aa
UniProt ID	Q6J2F7
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.80E-07, Aligned region: 9..267
Note	relaxase
Protein sequence [Download]	MLIRVSGYNTGAQEYLEKGNKSGREFTRDELDHRLIIEGQLSLTRAIYESIPDYGQDRYL TFTLSFKEDTVSPELLKSITTDFKNFFMHAYKPEEFNLYAEAHLPKMKTVTDRKTGEVID RKPHIHIIIPRINLLSGNEANPVDVYKNHEKYFEAIQEHINQKYGLSSPRENVRADITDA ASVLSRYKGDDFYGKNRQFKQELVKQVIERGVTSRADFYALVAEHGETRIRNEGKDTEYI SVKLPGDAKGTNLKDTIFQDDFIVRRELKKPPLEASVIQERLLAWPQRAREIKYVNKATP KFRKTYSEASPEDRVRLLADREAKFYQVHGEHNDNVHTGQRQRDHQRSPAETAGRRTAAP ADGVQDLSVSDVADHRQAGSARSRDGALLLPSDAHVHLGQSQPGGDSGLRPPVPGGGRGR RNGSTAERGRGGSGPAAVSQEATGTATPAGATGRRRAGAGKPRNARVRAGRIVPPYAKNP HRVATIADIEERGRRLFDPLKKPSDNALVFYRTPSTPSNTPATATTTSGRSSAGRKPRTS GKPRPPRQWRPGSVVPPYAKNPHRVATVADIEQRARMLFDPLKRPADKALVFKRASIKAL TVNRQASTVAAYFTREAQHNRIAPAHRQAIRRIDQQYFALRRAVFSDQRLTRQDKAQLVS VLTFERLKAREQFHKPQPNTEVNFMGSAAIRNLLEDEKEDPGFSISGARGPGPEGVRDRV KRVMDRFAKQVDPAAASQRARDLSAKDLYTRKAKFSQNVHYLDKQTDKTLFVDTGKTISM RRTGITESGVSVALQLARERFGSTLTITGTADFKKLVIEAVAKNGLDVHFTDKAMNHSLA ARRAELDIERGGQSIGPATDLPRHVDDSTRDVRDQADRLAVTVPIEALYGEGKTAEQVSQ ALAAQLDTVPEPERIAFVELVAITLGIPERGEPKGDQAFAQWQAQRAQPAADVAATATSE AQANVPTPSDAAPEPVSPEAAKPARVNDPDLQSPSELVRREAQWRRDFPMSEADVRASDT VMGLRGEDHAIWIIATDDKTPEAAAMLTAYMENDSYREAFKSSLETAYRQVENWPQAVQD LDQITEMAVKIVQQVEARLYPAPQAASGQATPSRNKVIEGELLDHGPAPYQHKDDKQMSY FVTLKPEGGKPRTVWGVGLEDAMKDSNLKQGDQVRLEDLGTQPVVVQVIEEDGTVTDKTV NRREWSAQPTAPEREVAETTPKGQAAAAGTPELSSPDEEDGMSVD

Reference

[1] Stavrinides J et al (2004) Nucleotide sequence and evolution of the five-plasmid complement of the phytopathogen Pseudomonas syringae pv. maculicola ES4326. J Bacteriol. 186(15):5101-15. [PMID:15262947]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	212	A46_pPMA4326A	YP_025664	241 aa	relaxosome component

Information of T4CP

ID	146
Name	VirD4_pPMA4326A
SecReT4 accesion number	_
GenBank accession number	YP_025656
Family	VirD4/TraG
Length	557
UniProt ID	Q6J2G6
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 4.90E-72, Aligned region: 4..482 TraG-D_C [PF12696.6], Evalue: 5.00E-22, Aligned region: 313..439 TrwB_AAD_bind [PF10412.8], Evalue: 7.40E-12, Aligned region: 271..459
Note	similar to Pseudomonas syringae pv. syringae A2 plasmid pPSR1 VirD4 encoded by GenBank Accession Number AY342395
Protein sequence [Download]	MGKAKQQPISPDNPQERIEEHPAILIGKHPTKDTFLASYGQTFVMLAAPPGTGKTVGVVT PNLLSYPDSVVVNDPKFENWRDTAGFRAAAGHKVYRFSPELLETHRWNPLSALSRDPLYR LGQIRTLAGVLFVSDNPKNQEWYNKAANVFAAILLYLMEMEGMKLNGMKLTLPQAYEVAS LGTGLGVWAQQAIEQHSTGPNALSVETLRELNGVFEASKNKSSGWSTTVDILRGALSMYA EKTVAWAVSDTDIDFTKLRKEKISIYFCVTGNAIKKYGPLMNLFFTQAIQQNTDVMPEQG GHCADGTLILKYQVLFVIDEIAVMGRIEIMEMAPALTRGAGLRYIIIFQGKDQLRSERTY GREAGNGIMQAFHIEIVYGTGNVELAKEYSTRLGNTTVRVHNQSLNRQKHEIGARGQTDS YSEQPRPLLLPQEVSALPYGEELIFVEATKTTPAANIRARKIFWYEEPVFKERAGMPTPD VPIGDASKIDALTVPVRTVEAKVAVADTKPMQAEQRQRWNPKDKASEVAQAEAAKAQPPE VEPDPEPAQADDTPETM

Reference

[1] Stavrinides J et al (2004) Nucleotide sequence and evolution of the five-plasmid complement of the phytopathogen Pseudomonas syringae pv. maculicola ES4326. J Bacteriol. 186(15):5101-15. [PMID:15262947]

Information of plasmid

ID	146
Plasmid name	pPMA4326A
GenBank accession number	NC_005918.1
Incompatibility group	IncI
Genome size	46697 bp
Coordinate of oriT [Strand]	39285..39370 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Pseudomonas syringae pv. maculicola strain ES4326 [59511]

Reference
N/A