oriTDB

The information of the oriT region

oriTDB accession number	100151
Name	oriT_pColK-K235
Sequence Completeness	incomplete
oriT length	33 nt
oriT sequence	GTAGCGATAGCGGAGTGTATACTGGCTTAATCA
IR (inverted repeat)	_
Location of nic site	[25-26] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	ColE1 superfamily
	D:25-26

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTAGCGATAGCGGAGTGTATACTGGCTTAATCA

Reference

[1] Rijavec M et al (2007) Prevalence of ColE1-like plasmids and colicin K production among uropathogenic Escherichia coli strains and quantification of inhibitory activity of colicin K. Appl Environ Microbiol. 73(3):1029-32. [PMID:17122402]

I. Information of Relaxase

ID	145
Name	MbkA_pColK-K235
GenBank accession number	YP_214176
Family	MOB_P
Length	532 aa
UniProt ID	Q58P29
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.40E-18, Aligned region: 49..247
Note	relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGARVLRGAPEEVRELIDATPFAKKYTSGVLSF AEQTLPPGERERVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELASGKRL QPYYDRADRPRINAWQTLVNHHYGLHDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEA LYHAGALKTRQDVTEALTAAGFEVVRTTRSSISIADPEGGRNLRLKGALYEQSFTNGDRL REETERACRLYREHAEQRVQEARSVCRQGTELKRAENQRRYQRTHSSGERTTGDAEQCRE RTVQRHRERTGPAAGRAALEAGQRADTVPVADGALRHGRDFSGVMGADSVAGKPDCGKHP GYQGPGRDAGETPGKNMGCDVSRGQEREISGAAVRGEGGKQLDGGEEERSKTGQGVSEYD RAGNALTERLRAIAAGLYAAAERLGEPLHGFAAYVRADTTGERATEGTVRRLESAGAEIS RAGGPLESVIYHEQQIRDEQERQARLERERARSWHEEQEREARVWRGPSIGM

Reference

[1] Rijavec M et al (2007) Prevalence of ColE1-like plasmids and colicin K production among uropathogenic Escherichia coli strains and quantification of inhibitory activity of colicin K. Appl Environ Microbiol. 73(3):1029-32. [PMID:17122402]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	210	MbkB_pColK-K235	YP_214177	186 aa	MbeB-like, N-term conserved region; pfam04837
2	211	MbkC_pColK-K235	YP_214175	67 aa	mobilization protein

N/A

Information of plasmid

ID	145
Plasmid name	pColK-K235
GenBank accession number	NC_006881.1
Genome size	8318 bp
Coordinate of oriT [Strand]	5471..5503 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli [562]

Reference
N/A