 oriTDB accession number | 100148 |
| Name | oriT_pSMA23  |
Sequence Completeness  | core |
| oriT length | 28 nt |
| oriT sequence | AGGTAAAGTATATTGGGCTATACCTTAC |
| IR (inverted repeat) | [3-12] [20-29] nt (GTAAAGTATA..TATACCTTAC) |
| Location of nic site | [16-17] nt |
| Conserved sequence flanking the nic site | TATTGG|GCTAT |
| Note | pMV158 superfamily; nick site (TGG|G) |
Visualization of oriT structure Reference| [1] Sudhamani M et al (2008) Characterisation of pSMA23, a 3.5 kbp plasmid of Lactobacillus casei, and application for heterologous expression in Lactobacillus. Plasmid. 59(1):11-9. [PMID:17961648] |
| ID | 142 |
| Name | Mob_pSMA23 |
| GenBank accession number | YP_001649176 |
| Family | MOBV |
| Length | 364 aa |
| UniProt ID | Q45TE0 |
| PDB ID | _ |
| Pfam | Mob_Pre [PF01076.18], Evalue: 3.40E-72, Aligned region: 1..195 |
| Note | clade A of the pMV158-superfamily of relaxases |
| Protein sequence [Download] | MSYLVANMQKLKADNLVGLGNHDQRRTQHHKNPDIDVDRSALNYDLVAGRTGHFKKTDIE AYINEHKTSQRAVRKDAVLVNEWIISSDSHFFADLTAADMRKYFETAKDYFAEKFGEENI RYAIVHLDESTPHMHMGIVPFDDEHKLSAKRVFNRTALRDVQDQLPAYLQQHGFNIQRGV QESERKSLTVPEYKAMRESIKQSQQKLAAVENETKQRQAKLKTYQATKFDVNSVKTKESR FHKRYVLVDRFDFDKLKQGASLTDTYFTETLSQRSDMDIQKDQLIKAESKAMELDIENRR LQKLVGTLQGIVRSVDRFLQRKLGVGLPSEWLERAGLKEPSKKAPQRPQERSEGQHDKLD GPSL |
Reference| [1] Sudhamani M et al (2008) Characterisation of pSMA23, a 3.5 kbp plasmid of Lactobacillus casei, and application for heterologous expression in Lactobacillus. Plasmid. 59(1):11-9. [PMID:17961648] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 207 | _ |
| ID | 142 |
| Plasmid name | pSMA23 |
| GenBank accession number | NC_010242.1 |
| Genome size | 3497 bp |
| Coordinate of oriT [Strand] | 529..556 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Lactobacillus casei A23 [1582] |
Reference