oriTDB

The information of the oriT region

oriTDB accession number	100148
Name	oriT_pSMA23
Sequence Completeness	core
oriT length	28 nt
oriT sequence	AGGTAAAGTATATTGGGCTATACCTTAC
IR (inverted repeat)	[3-12] [20-29] nt (GTAAAGTATA..TATACCTTAC)
Location of nic site	[16-17] nt
Conserved sequence flanking the nic site	TATTGG\|GCTAT
Note	pMV158 superfamily; nick site (TGG\|G)
	L:3-12;R:20-29;D:16-17

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGGTAAAGTATATTGGGCTATACCTTAC

Reference

[1] Sudhamani M et al (2008) Characterisation of pSMA23, a 3.5 kbp plasmid of Lactobacillus casei, and application for heterologous expression in Lactobacillus. Plasmid. 59(1):11-9. [PMID:17961648]

I. Information of Relaxase

ID	142
Name	Mob_pSMA23
GenBank accession number	YP_001649176
Family	MOB_V
Length	364 aa
UniProt ID	Q45TE0
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 3.40E-72, Aligned region: 1..195
Note	clade A of the pMV158-superfamily of relaxases
Protein sequence [Download]	MSYLVANMQKLKADNLVGLGNHDQRRTQHHKNPDIDVDRSALNYDLVAGRTGHFKKTDIE AYINEHKTSQRAVRKDAVLVNEWIISSDSHFFADLTAADMRKYFETAKDYFAEKFGEENI RYAIVHLDESTPHMHMGIVPFDDEHKLSAKRVFNRTALRDVQDQLPAYLQQHGFNIQRGV QESERKSLTVPEYKAMRESIKQSQQKLAAVENETKQRQAKLKTYQATKFDVNSVKTKESR FHKRYVLVDRFDFDKLKQGASLTDTYFTETLSQRSDMDIQKDQLIKAESKAMELDIENRR LQKLVGTLQGIVRSVDRFLQRKLGVGLPSEWLERAGLKEPSKKAPQRPQERSEGQHDKLD GPSL

Reference

[1] Sudhamani M et al (2008) Characterisation of pSMA23, a 3.5 kbp plasmid of Lactobacillus casei, and application for heterologous expression in Lactobacillus. Plasmid. 59(1):11-9. [PMID:17961648]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	207	_

N/A

Information of plasmid

ID	142
Plasmid name	pSMA23
GenBank accession number	NC_010242.1
Genome size	3497 bp
Coordinate of oriT [Strand]	529..556 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactobacillus casei A23 [1582]

Reference
N/A