oriTDB

The information of the oriT region

oriTDB accession number	100147
Name	oriT_pH205
Sequence Completeness	intact
oriT length	218 nt
oriT sequence	GATGGCATGGGGTGATATCCAGTAGCGGAGAGGTTGTTAGCGGGTTTCGGGGCGCAGCCC TGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTAAATATGTTACGGCGGAGTGG TGATTTATGAAAGTGCTTCATGTGACAAGGGAAAAAATGCGGCTACCGGGCGTAAGTGCA ACATGCAGGTAAGGATGAAATGACGCCTCCTCGCTCAC
IR (inverted repeat)	IR1: [46-53] [57-64] nt (TTCGGGGC..GCCCTGAA) IR2: [77-80] [81-84] nt (CGCT..AGCG)
Location of nic site	[97-98] nt
Conserved sequence flanking the nic site	CTGG\|CTAA
Note	ColE1 superfamily
	L:46-53;R:57-64;L:77-80;R:81-84;D: 97-98

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GATGGCATGGGGTGATATCCAGTAGCGGAGAGGTTGTTAGCGGGTTTCGGGGCGCAGCCC

61

TGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTAAATATGTTACGGCGGAGTGG

121

TGATTTATGAAAGTGCTTCATGTGACAAGGGAAAAAATGCGGCTACCGGGCGTAAGTGCA

181

ACATGCAGGTAAGGATGAAATGACGCCTCCTCGCTCAC

Reference

[1] Zioga A et al (2009) CMY-31 and CMY-36 cephalosporinases encoded by ColE1-like plasmids. Antimicrob Agents Chemother. 53(3):1256-9. [PMID:19104021]

I. Information of Relaxase

ID	141
Name	MobB_pH205
GenBank accession number	YP_001649335
Family	MOB_P
Length	156 aa
UniProt ID	B0FJX9
PDB ID	_
Pfam
Note	relaxase
Protein sequence [Download]	MQQAIRMRIEADSASGKMTETVRQSVNAALTQVEKELKQTGLEQQKPATDAFNQAAGTAK AMIHEMRREMSRYTWKSAIYIAMTMIAVMGGCLWGMYYFIDSGYARVAEMQHMEAVWQKK APLADIKTCDGNPCVKVDISRTFGDKKDTYLIIKGK

Reference

[1] Zioga A et al (2009) CMY-31 and CMY-36 cephalosporinases encoded by ColE1-like plasmids. Antimicrob Agents Chemother. 53(3):1256-9. [PMID:19104021]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	206	_

N/A

Information of plasmid

ID	141
Plasmid name	pH205
GenBank accession number	NC_010261.1
Genome size	8197 bp
Coordinate of oriT [Strand]	7094..7311 [-]
Drug resistance	CMY-31 cephalosporinase (bla_CMY-type gene)
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Klebsiella pneumoniae HP205 [573]

Reference

[1] Zioga A et al (2009) CMY-31 and CMY-36 cephalosporinases encoded by ColE1-like plasmids. Antimicrob Agents Chemother. 53(3):1256-9. [PMID:19104021]