oriTDB
The information of the oriT region
oriTDB accession number 100147
Name oriT_pH205  insolico
Sequence Completeness intact
oriT length 218 nt
oriT sequence GATGGCATGGGGTGATATCCAGTAGCGGAGAGGTTGTTAGCGGGTTTCGGGGCGCAGCCC TGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTAAATATGTTACGGCGGAGTGG TGATTTATGAAAGTGCTTCATGTGACAAGGGAAAAAATGCGGCTACCGGGCGTAAGTGCA ACATGCAGGTAAGGATGAAATGACGCCTCCTCGCTCAC
IR (inverted repeat)IR1: [46-53] [57-64] nt  (TTCGGGGC..GCCCTGAA)
IR2: [77-80] [81-84] nt  (CGCT..AGCG)
Location of nic site [97-98] nt
Conserved sequence flanking the
  nic site 		CTGG|CTAA
Note ColE1 superfamily
L:46-53;R:57-64;L:77-80;R:81-84;D: 97-98
Visualization of oriT structure
Reference
[1] Zioga A et al (2009) CMY-31 and CMY-36 cephalosporinases encoded by ColE1-like plasmids. Antimicrob Agents Chemother. 53(3):1256-9. [PMID:19104021]
I. Information of Relaxase
ID 141
Name MobB_pH205 insolico
GenBank accession number YP_001649335
Family MOBP
Length 156 aa
UniProt ID B0FJX9
PDB ID _
Pfam
Note relaxase
Protein sequence [Download] MQQAIRMRIEADSASGKMTETVRQSVNAALTQVEKELKQTGLEQQKPATDAFNQAAGTAK
AMIHEMRREMSRYTWKSAIYIAMTMIAVMGGCLWGMYYFIDSGYARVAEMQHMEAVWQKK
APLADIKTCDGNPCVKVDISRTFGDKKDTYLIIKGK
Reference
[1] Zioga A et al (2009) CMY-31 and CMY-36 cephalosporinases encoded by ColE1-like plasmids. Antimicrob Agents Chemother. 53(3):1256-9. [PMID:19104021]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 206 _ insolico
 N/A
Information of plasmid
ID 141
Plasmid name pH205
GenBank accession number NC_010261.1
Genome size 8197 bp
Coordinate of oriT  [Strand] 7094..7311 [-]
Drug resistance experimental CMY-31 cephalosporinase (blaCMY-type gene)
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Klebsiella pneumoniae HP205 [573]
Reference
[1] Zioga A et al (2009) CMY-31 and CMY-36 cephalosporinases encoded by ColE1-like plasmids. Antimicrob Agents Chemother. 53(3):1256-9. [PMID:19104021]

Microbial Bioinformatics Group in MML, SJTU