oriTDB

The information of the oriT region

oriTDB accession number	100146
Name	oriT_pTer331
Sequence Completeness	incomplete
oriT length	42 nt
oriT sequence	TATACCGGCGATTAGCCTATCCTGCAATAGCCCACACCCCCC
IR (inverted repeat)	_
Location of nic site	[24-25] nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note	The putative oriT site with 100% identity to that described for pIPO2 (Tauch et al., 2002).
	D:24-25

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TATACCGGCGATTAGCCTATCCTGCAATAGCCCACACCCCCC

Reference

[1] Mela F et al (2008) Comparative genomics of the pIPO2/pSB102 family of environmental plasmids: sequence, evolution, and ecology of pTer331 isolated from Collimonas fungivorans Ter331. FEMS Microbiol Ecol. 66(1):45-62. [PMID:18355297]

I. Information of Relaxase

ID	140
Name	TraR/VirD2_pTer331
GenBank accession number	YP_001672039
Family	MOB_P
Length	364 aa
UniProt ID	B0ZB68
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 3.70E-11, Aligned region: 73..302
Note	nickase/relaxase activity
Protein sequence [Download]	MSKTVIDGIIKDWGERLHYSPVKGSKGRNIRSGGTTRAAKPAPPSGPATKEKVARTAKKT AEVMVKISGGGKNMKHIKAHMDYISRNGEVEIEDENGDIHQGMEAVRDVRDSWAKGKIGI PYEGEKRKEAFNIVLSMPPGTDRQAVKDAAREFAKQEFGNHQYVFAAHDDEKHPHVHLAV KAVGNDGIRLNPRKGDLQFWREQFAEKLRDQGIEANATPRRARGVVQKAEKQAVRHIDAE FERGKRQEPARVTRARKRDAEAEVTTGKKRANPAQDNISAARKDTQKAYGQIARALATGE AEDKQIALNIVRLVQTMPPVVTKHEALVQSLHGTGGKLAGREAEPKQPKRQEQGRAKPDD EQTR

Reference

[1] Mela F et al (2008) Comparative genomics of the pIPO2/pSB102 family of environmental plasmids: sequence, evolution, and ecology of pTer331 isolated from Collimonas fungivorans Ter331. FEMS Microbiol Ecol. 66(1):45-62. [PMID:18355297]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	205	_

Information of T4CP

ID	140
Name	TraN/VirD4_pTer331
SecReT4 accesion number	_
GenBank accession number	YP_001672044
Family	VirD4/TraG
Length	900
UniProt ID	B0ZB73
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 6.40E-34, Aligned region: 99..330 T4SS-DNA_transf [PF02534.13], Evalue: 3.00E-61, Aligned region: 344..651 TraG-D_C [PF12696.6], Evalue: 1.30E-21, Aligned region: 482..608 TrwB_AAD_bind [PF10412.8], Evalue: 4.80E-10, Aligned region: 387..624 Ribosomal_L12 [PF00542.18], Evalue: 1.60E-07, Aligned region: 299..365
Note	ATPase, coupling the relaxosome with the transfer machinery
Protein sequence [Download]	MEMPKWLKWVLGVVVFIAATVGAVWLAGFFFFAFSKTNPFGKTDFSTWWTYWQFYQADPV IAKRLKVSGIVAAVVAYGAPIVALIAAMREVRSLHGEARFANASEIEKAGLFGNTGIIIG KLKNRFLMFAGMQFVLLAAPTRSGKGVGVVIPNLLNWSESVVVLDVKLENFLITSKFRAK WGQEVYLFNPFSISEDAEGNPLNGRTHRYNPLGYIGDDARLRVTDILAIGYSLYPGEGRD AFFDDAARNLFLGLTLYLCETPSLPRTMGELLRQSSGKGKPVKQHIQGIVTERNFREFGD VMLNDVGEDQAEVVKAIMAAAGISEAEAEKLCDDAPIVVVKDVAAAEIEQVEALFKEAGA EVEVTRKLVPITQWDGVGLPPLTMECVDALNRFTATSDNTLSSIMASFNVPLTIWASPIV DAATSANDFDLRDVRKKRMSIYLGIPANKLAEAKLLMNMFYTQLVNLNTNRLLHSSPELK YTVLMLDDEFTAPGRIGIIDKANSYMAGYGLRMLNIIQSPGQLEAEAPKGYGRENARTFV TNHACQIFFTPREQRDANEYSEALGYYTFKAKGSSRQLGGKAAGGRSESESDQKRALMMP QELKEMSQREQIISLENTKAIRCEKISYFSDHVFVDRLKSVTPSLAKLGKKLPTKKQLED AWGSGECAVSIPLLNLDLHEAVVQARSRALTVTDVAKGIDLRALALDFSKVPLPTGDGIE AEQVEAFVDGFFDALDAANEYDEDEAPDVDEDGASERPSDDELAALDAEAADDGGKVAVN TIEQADDEPAAAELVTGASRPGGGSVAKPEPQAEPEPEPDRGLGHDQADIEPDGPTDEDL AAMMEDFTPPDDGMMDEADMLAALDEMEAVPEYVEEAENDAPILDLSVLDKPLPSIKNAK

Reference

[1] Mela F et al (2008) Comparative genomics of the pIPO2/pSB102 family of environmental plasmids: sequence, evolution, and ecology of pTer331 isolated from Collimonas fungivorans Ter331. FEMS Microbiol Ecol. 66(1):45-62. [PMID:18355297]

Information of plasmid

ID	140
Plasmid name	pTer331
GenBank accession number	NC_010332.1
Incompatibility group	PromA
Genome size	40457 bp
Coordinate of oriT [Strand]	10770..10811 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Collimonas fungivorans Ter331 [1005048]

Reference
N/A