oriTDB
The information of the oriT region
oriTDB accession number 100146
Name oriT_pTer331  insolico
Sequence Completeness incomplete
oriT length 42 nt
oriT sequence TATACCGGCGATTAGCCTATCCTGCAATAGCCCACACCCCCC
IR (inverted repeat)_
Location of nic site [24-25] nt
Conserved sequence flanking the
  nic site 		TATCCTG|C
Note The putative oriT site with 100% identity to that described for pIPO2 (Tauch et al., 2002).
D:24-25
Visualization of oriT structure
Reference
[1] Mela F et al (2008) Comparative genomics of the pIPO2/pSB102 family of environmental plasmids: sequence, evolution, and ecology of pTer331 isolated from Collimonas fungivorans Ter331. FEMS Microbiol Ecol. 66(1):45-62. [PMID:18355297]
I. Information of Relaxase
ID 140
Name TraR/VirD2_pTer331 insolico
GenBank accession number YP_001672039
Family MOBP
Length 364 aa
UniProt ID B0ZB68
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 3.70E-11, Aligned region: 73..302
Note nickase/relaxase activity
Protein sequence [Download] MSKTVIDGIIKDWGERLHYSPVKGSKGRNIRSGGTTRAAKPAPPSGPATKEKVARTAKKT
AEVMVKISGGGKNMKHIKAHMDYISRNGEVEIEDENGDIHQGMEAVRDVRDSWAKGKIGI
PYEGEKRKEAFNIVLSMPPGTDRQAVKDAAREFAKQEFGNHQYVFAAHDDEKHPHVHLAV
KAVGNDGIRLNPRKGDLQFWREQFAEKLRDQGIEANATPRRARGVVQKAEKQAVRHIDAE
FERGKRQEPARVTRARKRDAEAEVTTGKKRANPAQDNISAARKDTQKAYGQIARALATGE
AEDKQIALNIVRLVQTMPPVVTKHEALVQSLHGTGGKLAGREAEPKQPKRQEQGRAKPDD
EQTR
Reference
[1] Mela F et al (2008) Comparative genomics of the pIPO2/pSB102 family of environmental plasmids: sequence, evolution, and ecology of pTer331 isolated from Collimonas fungivorans Ter331. FEMS Microbiol Ecol. 66(1):45-62. [PMID:18355297]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 205 _ insolico
Information of T4CP
ID 140
Name TraN/VirD4_pTer331 insolico
SecReT4 accesion number _
GenBank accession number YP_001672044
Family VirD4/TraG
Length 900
UniProt ID B0ZB73
PDB ID _
Pfam T4SS-DNA_transf [PF02534.13], Evalue: 6.40E-34, Aligned region: 99..330
T4SS-DNA_transf [PF02534.13], Evalue: 3.00E-61, Aligned region: 344..651
TraG-D_C [PF12696.6], Evalue: 1.30E-21, Aligned region: 482..608
TrwB_AAD_bind [PF10412.8], Evalue: 4.80E-10, Aligned region: 387..624
Ribosomal_L12 [PF00542.18], Evalue: 1.60E-07, Aligned region: 299..365
Note ATPase, coupling the relaxosome with the transfer machinery
Protein sequence [Download] MEMPKWLKWVLGVVVFIAATVGAVWLAGFFFFAFSKTNPFGKTDFSTWWTYWQFYQADPV
IAKRLKVSGIVAAVVAYGAPIVALIAAMREVRSLHGEARFANASEIEKAGLFGNTGIIIG
KLKNRFLMFAGMQFVLLAAPTRSGKGVGVVIPNLLNWSESVVVLDVKLENFLITSKFRAK
WGQEVYLFNPFSISEDAEGNPLNGRTHRYNPLGYIGDDARLRVTDILAIGYSLYPGEGRD
AFFDDAARNLFLGLTLYLCETPSLPRTMGELLRQSSGKGKPVKQHIQGIVTERNFREFGD
VMLNDVGEDQAEVVKAIMAAAGISEAEAEKLCDDAPIVVVKDVAAAEIEQVEALFKEAGA
EVEVTRKLVPITQWDGVGLPPLTMECVDALNRFTATSDNTLSSIMASFNVPLTIWASPIV
DAATSANDFDLRDVRKKRMSIYLGIPANKLAEAKLLMNMFYTQLVNLNTNRLLHSSPELK
YTVLMLDDEFTAPGRIGIIDKANSYMAGYGLRMLNIIQSPGQLEAEAPKGYGRENARTFV
TNHACQIFFTPREQRDANEYSEALGYYTFKAKGSSRQLGGKAAGGRSESESDQKRALMMP
QELKEMSQREQIISLENTKAIRCEKISYFSDHVFVDRLKSVTPSLAKLGKKLPTKKQLED
AWGSGECAVSIPLLNLDLHEAVVQARSRALTVTDVAKGIDLRALALDFSKVPLPTGDGIE
AEQVEAFVDGFFDALDAANEYDEDEAPDVDEDGASERPSDDELAALDAEAADDGGKVAVN
TIEQADDEPAAAELVTGASRPGGGSVAKPEPQAEPEPEPDRGLGHDQADIEPDGPTDEDL
AAMMEDFTPPDDGMMDEADMLAALDEMEAVPEYVEEAENDAPILDLSVLDKPLPSIKNAK

Reference
[1] Mela F et al (2008) Comparative genomics of the pIPO2/pSB102 family of environmental plasmids: sequence, evolution, and ecology of pTer331 isolated from Collimonas fungivorans Ter331. FEMS Microbiol Ecol. 66(1):45-62. [PMID:18355297]
Information of plasmid
ID 140
Plasmid name pTer331
GenBank accession number NC_010332.1
Incompatibility group PromA
Genome size 40457 bp
Coordinate of oriT  [Strand] 10770..10811 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Collimonas fungivorans Ter331 [1005048]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU