oriTDB

The information of the oriT region

oriTDB accession number	100145
Name	oriT_pCT14
Sequence Completeness	incomplete
oriT length	30 nt
oriT sequence	ATTGCGTATGGTGTATAGCTCGTTAAGGGA
IR (inverted repeat)	_
Location of nic site	[14-15] nt
Conserved sequence flanking the nic site	GGTGT\|ATAGC
Note	_
	D:14-15

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ATTGCGTATGGTGTATAGCTCGTTAAGGGA

Reference

[1] Bramucci M et al (2006) Genetic organization of a plasmid from an industrial wastewater bioreactor. Appl Microbiol Biotechnol. 71(1):67-74. [PMID:16244860]

I. Information of Relaxase

ID	139
Name	TraI_pCT14
GenBank accession number	YP_001966297
Family	MOB_F
Length	984 aa
UniProt ID	Q2QCQ9
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 2.00E-84, Aligned region: 12..293 AAA_30 [PF13604.5], Evalue: 1.50E-40, Aligned region: 494..681 Viral_helicase1 [PF01443.17], Evalue: 1.90E-05, Aligned region: 514..704 Viral_helicase1 [PF01443.17], Evalue: 6.70E-06, Aligned region: 792..916 UvrD_C_2 [PF13538.5], Evalue: 2.10E-07, Aligned region: 868..914
Note	relaxase
Protein sequence [Download]	MLDITTISRQSLGKVVSYYADGADDYYAKDGGAMQWQGAGAEALGLSGEVEQARFRELLD GRISDSTKLMRTVKEADGKVVSKERLGYDLTFSAPKGVSLQALVHGDASIIEAHDKAVAA AIREAERLSQARITVNKKTGTENTNNLVVAKFRHETSRALDPDLHTHAFVLNMTQRSDGE WRALKNDGVFNSSMFLGNVYKAELARELEKAGFQLRYERNGTFDLAHFSDEQIREFSSRS QQIEAALAAKGLDRSTASYAEKNQAALATRDKKQGGIDREELRQVWLERSRALGIDYHSR EWAGVGADAQGGRERNSAATPQIEKPLEYRADQVIEFAIKSLTERQAVIGQKELMDTALR HGYGALTIDDVRAGIERRAASGHLIREEPLYSSQNPADGKKGKAAEKAREEAPQLSRKEW VATLVRAGKSRSEAARLVDEGIRTGRLRQGENRFTTHIAQKREREVLQIERMGRGTVEPR ISKEAAEAFLADRGLKAEQQASVMRIARTQNQFIGVQGFAGVGKSYMTVAAKDLLEANGY RVTSLAPYGSQVKALQAEGLEARTLQSFLKARDKKIDSNTVVFIDEAGVIPARQMHEAMK TIEAAGARVVFLGDVAQTKAIEAGKPFEQLMKAGMETSRLTDIQRQKDPQLLEAVKLAAE GKAKQSLPLVNEIREIKEDGARYQAIVDAYAKMTKAERDQALIITGTNASRIQINEGVRE ALGLKGQGAEYPLLNRLDTTQAERRHSKYYGKGSIVVPEVDYKNGLQRGVQYVVLDTGPG NKLTVRGPEGETLQFTPARCTKLSVYSVERTELVAGDQVKITRNDAEKDLANGDRFVVKE IHSDRVVLEGDKGRQVELDTASAMFVGLAYASTVHSAQGLTCDKVLINLETQSRTTAKDV YYVAISRARHAAEIFTDNRQKLGDAISRLNAKAGALDIKQLQRHALERKGHDQAGKQKDA VQKQQQGQQLPAKQPKEKGRAFGL

Reference

[1] Bramucci M et al (2006) Genetic organization of a plasmid from an industrial wastewater bioreactor. Appl Microbiol Biotechnol. 71(1):67-74. [PMID:16244860]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	204	_

Information of T4CP

ID	139
Name	TraJ_pCT14
SecReT4 accesion number	_
GenBank accession number	YP_001966296
Family	TrwB/TraD
Length	512
UniProt ID	Q2QCR0
PDB ID	_
Pfam	TrwB_AAD_bind [PF10412.8], Evalue: 1.40E-101, Aligned region: 110..492 TraG-D_C [PF12696.6], Evalue: 5.50E-22, Aligned region: 349..473 T4SS-DNA_transf [PF02534.13], Evalue: 4.20E-09, Aligned region: 297..488 DUF87 [PF01935.16], Evalue: 5.90E-08, Aligned region: 101..241
Note	Type IV secretion-system coupling protein
Protein sequence [Download]	MNERQRSQICLAVMVLVPLFAWFFTAKLMYGIEKQGARDRVFYLAKNTFTLWPLAVALVG GLILAIALCVLIVKLSKTTFAGAHFDKYFRGSQLVSQRELKRLTKEKEEQITIAGVPVPI AAEATHFSVGGATGTGKSTIFREMMYGCMQRKDRMVILDPDGEFLSTFYRKGDKILNPYD ARTEGWNFYNEIRSHYDFERYAKSIIQVSDSNDSEEWNQYGRLLFAEVAKKLYNTTRNPG MREVFRWTNECDSETLQAFVKGTRAVSLFTENERATGSVRFVLSNKLPAHFDMPPGNFSL RQWCEDPNGGNLFITWDENMREALRPLISCWVDTIFTSILGMKSNPKRRIWTFLDELESL QRLPTLGDMLTRGRKKGGCVVSGYQSYTQLESVYGEKLAETMLANHRTMVALAVGRMGTA TAERMSKALSEHEVLRTKEGRSSRWGDWGTRSENEDVKPERIVMSSEIMALNNLEGFLSF PGSIPIARVTIDPINFTRTNPVPGIVPNLEII

Reference

[1] Bramucci M et al (2006) Genetic organization of a plasmid from an industrial wastewater bioreactor. Appl Microbiol Biotechnol. 71(1):67-74. [PMID:16244860]

Information of plasmid

ID	139
Plasmid name	pCT14
GenBank accession number	NC_010891.1
Incompatibility group	IncN-like
Genome size	55216 bp
Coordinate of oriT [Strand]	17343..17372 [-]
Drug resistance	_
Heavy-metal resistance	mercury resistance
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Pseudomonas sp. CT14 [341029]

Reference

[1] Bramucci M et al (2006) Genetic organization of a plasmid from an industrial wastewater bioreactor. Appl Microbiol Biotechnol. 71(1):67-74. [PMID:16244860]