oriTDB

The information of the oriT region

oriTDB accession number	100144
Name	oriT_pTiBo542
Sequence Completeness	incomplete
oriT length	14 nt
oriT sequence	CAAGGGCGCAATTA
IR (inverted repeat)	_
Location of nic site	[6-7] nt
Conserved sequence flanking the nic site	_
Note	Ti plasmid
	D:6-7

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CAAGGGCGCAATTA

Reference

[1] Wetzel ME et al (2015) The repABC Plasmids with Quorum-Regulated Transfer Systems in Members of the Rhizobiales Divide into Two Structurally and Separately Evolving Groups. Genome Biol Evol. 7(12):3337-57. [PMID:26590210]

I. Information of Relaxase

ID	138
Name	TraA_pTiBo542
GenBank accession number	AAZ50586
Family	MOB_Q
Length	1100 aa
UniProt ID	A5WYB8
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 9.40E-76, Aligned region: 17..243 AAA_30 [PF13604.5], Evalue: 7.00E-55, Aligned region: 381..569 AAA_19 [PF13245.5], Evalue: 1.20E-09, Aligned region: 386..513 Viral_helicase1 [PF01443.17], Evalue: 9.60E-05, Aligned region: 656..737 UvrD_C_2 [PF13538.5], Evalue: 1.90E-06, Aligned region: 692..736
Note	Dtr system oriT relaxase; Provisional
Protein sequence [Download]	MAIAHFSASIISRGDGRSVVLSAAYRHCAKMEYEREARTIDYTRKQGLLHEEFTLPADAP KWAKALIADRAVSGAVEAFWNKVEAFEKRSDAQLARDLTIALPLELTPEQNIALVRDFVE KHIRAKGMVADWVYHDNPGNPHVHLMSTLRPLSEDGFGSKKVAVIGEDGQPVRTKSGKIL YELWGGSTDAFNVLRDGWFERQNHHLALAEIDLRIDGRSYEKQGIELEPTIHLGVGAKAI ERKAASRGVRPELERIELNEERRSENARRILRNPAIVLDLITREKSVFDERDIAKVLHRY IDDPAVFQQLMVRIILNPEVLRLQRETIDFATAKKVPARYSTRAMIRLEATMARQAMWLS NRELRGVSPSALNATFGRHARLSDEQKAAIERIAGPARIAAVVGRAGAGKTTMMKAAREA WELAGYRVVGGALAGKAAEGLSSEAGIESRTLSSWELRWNRGRDVLDNKTVFVMDEAGMV ASKQMAGFVDAVVRAGAKIVLVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQREDWM RKASLDLARGNVEKALALYNANAGIVGERLKAEAVERLIAHWNHDYDQTKTTLILAHLRR DVRMLNVMAREKLVERGIVGEGHVFRTADGERRFDAGDQIVFLKNETSLGVKNGMIGHVV EAAPNRIVAVVGDRDHRRHVVVEQRFYRNLDHGYATTIHKSQGATVDRVKVLASLSLDRH LTYVAMTRHREDLQLYYGRRSFAFNGGLAKVLSRKNAKETTLDYERGKLYREALRFAENR GLHIMQVARTMLRDRLDWTLRQKTKVSDLVHRLRALGERLGPDQSPKTQTMKEAGPMVAG IKTFSGSVADTVGDKLGADPTLKQQWEEVSARFRYVFADPVTAFRAINFDTVLADKEMAK QVLQKLEAEPETIGPLKGKTGILAGKAEREARRIAEVNVPALKRDLEQYLQMRETATMRH QADEQTLRQRVSIDIPALSPAARVVLERVRDAIDRNDLPAAMAYALSNRETKLEIDGFSQ SVTERFGERTLLNNAAREPSGTLYEKLSEGMTPEQKQQLKQAWPVMRTAQQLAAHERTAQ SLRQAEEQRLTQRQVPVLKQ

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	202	TraC_pTiBo542	AAZ50585	98 aa	conjugal transfer protein TraC
2	203	TraD_pTiBo542	AAZ50584	71 aa	conjugal transfer protein TraD

N/A

Information of plasmid

ID	138
Plasmid name	pTiBo542
GenBank accession number	DQ058764.1
Genome size	244978 bp
Coordinate of oriT [Strand]	213650..213663 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	virulence-tumorigenic
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Agrobacterium tumefaciens Bo542 [358]

Reference

[1] Wetzel ME et al (2014) Quorum-dependent mannopine-inducible conjugative transfer of an Agrobacterium opine-catabolic plasmid. J Bacteriol. 196(5):1031-44. [PMID:24363349]

[2] Wetzel ME et al (2015) The repABC Plasmids with Quorum-Regulated Transfer Systems in Members of the Rhizobiales Divide into Two Structurally and Separately Evolving Groups. Genome Biol Evol. 7(12):3337-57. [PMID:26590210]