oriTDB

The information of the oriT region

oriTDB accession number	100143
Name	oriT_pMG2200
Sequence Completeness	intact
oriT length	322 nt
oriT sequence	AGAACAATCAACAACTAATTAGGCAAATTAAGGGGTGCTAAACAACTGCTAGTAGGTGCT AGAGATGTGCTATAAAGGGTGCTAGTTTGGTGCTAGTTACTGCTAAATACGTGCTAGTTT AGGTGCTAGAAACGTGCTATATGGTGCTAAAAAGGTGCTAGTTTGCATGAAGTTACCTGC TAGCCAAGTGCTAGTGGCGTTCGTTTTTGGGTCCCACGGGAAAGCCTTGCACTGCAAGGC GGGTCAGCTTGTCTGACCCCCATTTCCCCTTATGCTCTTCCGAAACACAAAGCGCAATTA AGCGAATACTAGAGAATAAATA
IR (inverted repeat)	IR1: [224-231] [233-240] nt (GCCTTGCA..TGCAAGGC) IR2: [241-247] [253-259] nt (GGGTCAG..CTGACCC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	_
	L:224-231;R:233-240;L:241-247;R:253-259

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGAACAATCAACAACTAATTAGGCAAATTAAGGGGTGCTAAACAACTGCTAGTAGGTGCT

61

AGAGATGTGCTATAAAGGGTGCTAGTTTGGTGCTAGTTACTGCTAAATACGTGCTAGTTT

121

AGGTGCTAGAAACGTGCTATATGGTGCTAAAAAGGTGCTAGTTTGCATGAAGTTACCTGC

181

TAGCCAAGTGCTAGTGGCGTTCGTTTTTGGGTCCCACGGGAAAGCCTTGCACTGCAAGGC

241

GGGTCAGCTTGTCTGACCCCCATTTCCCCTTATGCTCTTCCGAAACACAAAGCGCAATTA

301

AGCGAATACTAGAGAATAAATA

Reference

[1] Zheng B et al (2009) Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac). Antimicrob Agents Chemother. 53(2):735-47. [PMID:19029325]

I. Information of Relaxase

ID	137
Name	ORF44_pMG2200
GenBank accession number	YP_002333406
Family	MOB_P
Length	686 aa
UniProt ID	B6ZHK5
PDB ID	_
Pfam
Note	putative relaxase (plasmid)
Protein sequence [Download]	MNKKTAGIILTSAFVMPGTDTFKEYLDYMERSEATRNEHFDQFNAFSPDNSMDSIAAVEG SNETMFSTYNDYMSNPKKTSALFTNNYDQAPEEVIRYMKEYFEMAQENKSPLWQLVFSFR NEWLIENNYLDPETNQLKTQHIYQATRLAIAELEKKEGLKGEWTGAVHYNTDNIHVHVGY VEKNPTREWIFYKHPKEPSKTGWQFKGKFLQKSIKATKRTFVNELMQNKEQLSMLETYVT HQKKLAESCKEQFTQGKYEQLFTDLLQKLPSNQYFWKYGFAERHKFKPELDKIVDLFMET DGKETMDEILPRLQKISDDYEEAYGNPRNEPTYLDKQLYGKSGLYSRIGNMILQEAKNYP ELLAKRKTGLMNESDFEQFNFETEIESEGLIEPFDSLEMTMPQEENAEESMIYDDVLPHE LLEESHEEVNNIEEQIKVKQSENKMDQALKTLHDYFTEREESKDSRNLKIVDQLKAEKES SKKEIRGMFLHEVEGNQFAEPVSSFTHETLGRKIILPSKNKAHYISIETSKDKEVDSNLQ SFSGNNQRAILQQIPTATHVLGEKQWQLNNRQIREDEIENAIKIVTIKKDESGSFVFETK DVYDISQTDKLPKKERNANYKIEKATEKNGQKRVTKVSNRKPVVSHKKSDYEFKKQLRQL RKSCERSVQRYLNEKAYQELEQSMNI

Reference

[1] Zheng B et al (2009) Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac). Antimicrob Agents Chemother. 53(2):735-47. [PMID:19029325]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	201	_

Information of T4CP

ID	137
Name	VirD4_pMG2200
SecReT4 accesion number	_
GenBank accession number	YP_002333362
Family	VirD4/TraG
Length	843
UniProt ID	B6ZHS5
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 7.00E-22, Aligned region: 119..655 TraG-D_C [PF12696.6], Evalue: 4.70E-17, Aligned region: 513..633
Note	coupling protein (plasmid)
Protein sequence [Download]	MKKKEKGKFKKKNYEKAKQNNLRFLADKRFLIPAMIFLFLTEIIVGNWLLQNIFSLLKSN LFSIQWVEFKETLFYYPLSEYLFAYILLFIASILSTSIIAYRMYTSFRSLEEEHVQGTMD FESTEGMDKQYPTVEVHPFTKTEDFYDGKPGYPVGRKPKTMKQKAEGTFSLYVDTTDSNT ITLAGTRQGKGIYFVDTFIDILTRARLIANRASFVMTATKGDEPRKWYNTLKRRGYRIRI ANVVNPYYSDPIPALAVFNNYYRHYKRLKKESVGLKEKGKASERLNVSIKAEQQLSNAEK IIKQVAFSYFREVNEGKDGGFWTKACRSLFMSVGLALADQEYEENETSKVNPYTIYNVVN DMQRRRIKENSHEFLKKYSNDENELQRLLAEYENKSELDVFFGELPRTHPAKRHYDAIFA SAPAHVTLGNIITHFDGDLEPFLMTLNAKMTAIDDGFDMELVGFDREQPTAVFVMLSDAD TSNNQLGVMYLEQIYQVLLNRCNLEDDSQCYRDVHFIYEEGGNLGVAISDLARKWTAGLS RRLFSHLVLQDIQQLSSLYDEDTKDIILGNTGNLAYIRTGSESTNKYISGRLGDRSNYSK TRHKDPLSIKSTETESTERIDLLASFELERLREGESVILRINKHRDLEGNPIYQYPIYNT YENDTNLIPFYKFRKLDKVSWEEIPVNNQFMEIELEDLNWKLQPGKIIDQQKQVIPQIPT YKKPERRKDSNSITPIEQIAKKEVKSVFSTEKYAKEAKFLEDMYGDKLEHLVINVFSVEQ QKRLIALIQMTGEKEPQSFAELERVSKTGNVRSYISCILSSFGQGAVNMVVQQLKEWRNS NEF

Reference

[1] Zheng B et al (2009) Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac). Antimicrob Agents Chemother. 53(2):735-47. [PMID:19029325]

Information of plasmid

ID	137
Plasmid name	pMG2200
GenBank accession number	NC_011642.1
Genome size	106527 bp
Coordinate of oriT [Strand]	43622..43943 [+]
Drug resistance	vancomycin resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterococcus faecalis [1351]

Reference

[1] Zheng B et al (2009) Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac). Antimicrob Agents Chemother. 53(2):735-47. [PMID:19029325]