oriTDB
The information of the oriT region
oriTDB accession number 100142
Name oriT_pLB925A02  insolico
Sequence Completeness incomplete
oriT length 23 nt
oriT sequence TAAAGTATATTGGGCTATACCTT
IR (inverted repeat)[3-9] [16-23] nt  (AAGTATA..TATACCTT)
Location of nic site _
Conserved sequence flanking the
  nic site
_
Note _
L:3-9;R:16-23
Visualization of oriT structure
Reference
[1] Wada T et al (2009) Characterization of four plasmids harboured in a Lactobacillus brevis strain encoding a novel bacteriocin, brevicin 925A, and construction of a shuttle vector for lactic acid bacteria and Escherichia coli. Microbiology. 155(Pt 5):1726-37. [PMID:19372160]
I. Information of Relaxase
ID 136
Name MobA_pLB925A02 insolico
GenBank accession number YP_002790952
Family MOBV
Length 361 aa
UniProt ID C0SQL3
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 6.40E-72, Aligned region: 1..194
Note putative plasmid mobilization protein
Protein sequence [Download] MSYLVANMQKLKADNLVGLGNHDQRRTQHHKNTDIEVDRSGLNYDLVAGRTNHFKTDIAA
YINEHKTSQRAVRKDAVLVDEWIISSDSNFFANLTAADTRKYFETAKAYFAEKFGEENVR
YAIVHLDESTPHMHMGIVPFDDEYKLSAKRVFNRAALQNVQDQLPTYLQQHGFNIQRGVQ
ESERKSLTVPEYKAMREDLKKATLQKQEIQAELEDARKRLAELKPRDQQEIESKPTFLSK
DKVVVRKSDLHDLESRAAVSDIYNQQQNRLKLDNQSLNYQLLEVKDNNYELSKKNEKLQK
LVDTLQGIVRSVDRFLQRKLGVGLPSEWLERAGLKEPSKNAPQRPQERSEGQHDELDGPS
L
Reference
[1] Wada T et al (2009) Characterization of four plasmids harboured in a Lactobacillus brevis strain encoding a novel bacteriocin, brevicin 925A, and construction of a shuttle vector for lactic acid bacteria and Escherichia coli. Microbiology. 155(Pt 5):1726-37. [PMID:19372160]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 200 _ insolico
 N/A
Information of plasmid
ID 136
Plasmid name pLB925A02
GenBank accession number NC_012549.1
Genome size 3524 bp
Coordinate of oriT  [Strand]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactobacillus brevis 925A [1580]
Reference
 N/A