oriTDB

The information of the oriT region

oriTDB accession number	100141
Name	oriT_pLB925A03
Sequence Completeness	core
oriT length	47 nt
oriT sequence	CGAAGCAAAATGTATGTCATTCCGACAGCTTGCATTATTTGTTTTTT
IR (inverted repeat)	[15-18] [24-27] nt (TGTC..GACA)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	_
	L:15-18;R:24-27

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGAAGCAAAATGTATGTCATTCCGACAGCTTGCATTATTTGTTTTTT

Reference

[1] Wada T et al (2009) Characterization of four plasmids harboured in a Lactobacillus brevis strain encoding a novel bacteriocin, brevicin 925A, and construction of a shuttle vector for lactic acid bacteria and Escherichia coli. Microbiology. 155(Pt 5):1726-37. [PMID:19372160]

I. Information of Relaxase

ID	135
Name	MobA_pLB925A03
GenBank accession number	YP_002790959
Family	MOB_P
Length	415 aa
UniProt ID	C0SQM0
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.80E-62, Aligned region: 9..280
Note	relaxase
Protein sequence [Download]	MATIAKVTNGASAASALDYALGKDKPLHEHTERWLEENRLERLDALKDCRAVAVGGTNGI DPVIAKEQFKAVQQAFNQTARRNQVLRITQSFALNELDPTNTRDWQRANDLGCELAEKLY PDYQTAVYTHLDGQNHILHNHIIINKVNLQTGKKLDERKGSAVERARNANDEISKEQGWK VIEPVREHQSRTEQDLTKKGQYSYMHDLRGRIDTTMQDTSICDFKTFSDVLARSGVNVSV RGQNVSYAFLDANKKQRRARGKRLGTDYEKETILNELERRTRERTAQQLDREDQSRATEL VRETTSTDTALEQRERQAQSRKPRIDQLRNAVKPIADHLQHFTGAVREFAGKVEQRVIDS KLYKDVASRFKADLAKQEQARQNAIKQDLAKRMAPKKQPQQPQRSYYREPEGRSR

Reference

[1] Wada T et al (2009) Characterization of four plasmids harboured in a Lactobacillus brevis strain encoding a novel bacteriocin, brevicin 925A, and construction of a shuttle vector for lactic acid bacteria and Escherichia coli. Microbiology. 155(Pt 5):1726-37. [PMID:19372160]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	198	MobB_pLB925A03	YP_002790960	222 aa	putative plasmid mobilization protein
2	199	MobC_pLB925A03	YP_002790958	112 aa	putative plasmid mobilization protein

N/A

Information of plasmid

ID	135
Plasmid name	pLB925A03
GenBank accession number	NC_012550.1
Genome size	8881 bp
Coordinate of oriT [Strand]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactobacillus brevis 925A [1580]

Reference
N/A