oriTDB

The information of the oriT region

oriTDB accession number	100140
Name	oriT2_pMAS2027
Sequence Completeness	incomplete
oriT length	19 nt
oriT sequence	TTGCCCTATCCTGCATCGC
IR (inverted repeat)	_
Location of nic site	[13-14] nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note	_
	D:13-14

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTGCCCTATCCTGCATCGC

Reference

[1] Ong CL et al (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl Environ Microbiol. 75(21):6783-91. [PMID:19717626]

I. Information of Relaxase

ID	134
Name	TaxC_pMAS2027
GenBank accession number	YP_003292208
Family	MOB_P
Length	390 aa
UniProt ID	D0QMQ2
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.40E-10, Aligned region: 66..309
Note	Relaxase
Protein sequence [Download]	MIMGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISG GAVTRQGIRNSIDYMSRESELPVMSESGRVWTGDEILEAKDHMIDRANDPQHVMNDKGKE NKKITQNIVFSPPVSAKVKPEDLLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRI RDNDGKRADIRKKDLREIRTGFCEELKLKGYDVKATHKQQHGLNQSVKDAHNTAPKRQKG VYEVVDVGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADFGDLCSRESVKAGDLVRLK KLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLNSPDMLLKQ QQRMAQFTQQKASTLQSEQKLKTGIKFWSL

Reference

[1] Ong CL et al (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl Environ Microbiol. 75(21):6783-91. [PMID:19717626]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	197	Ddp1_pMAS2027	YP_003292209	204 aa	similar to TaxA from R6K

Information of T4CP

ID	134
Name	TaxB_pMAS2027
SecReT4 accesion number	_
GenBank accession number	YP_003292195
Family	VirD4/TraG
Length	613
UniProt ID	D0QMN9
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 1.70E-143, Aligned region: 103..580 TraG-D_C [PF12696.6], Evalue: 2.90E-24, Aligned region: 417..537 TrwB_AAD_bind [PF10412.8], Evalue: 1.70E-16, Aligned region: 309..553
Note	bacterial conjugative protein involved in type IV sectretion
Protein sequence [Download]	MIMSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESN IRIDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEK ENDTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELW KITSKVREILLGNKVYLLDPFNSKTHQFNPLFYIDLKAESGAKDLLKLIEILFPSYGMTG AEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSIGSVVDLYSNIDRELILSK REELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYLPTVRKCTD GNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLM FLDEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIY AVSEEDDAAKISEKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEE FIILKGENPVKAEKALYYLDPYFMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEK MLSVGELESEVLL

Reference

[1] Ong CL et al (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl Environ Microbiol. 75(21):6783-91. [PMID:19717626]

Information of plasmid

ID	134
Plasmid name	pMAS2027
GenBank accession number	NC_013503.1
Incompatibility group	IncX1
Genome size	42644 bp
Coordinate of oriT [Strand]	2060..2078 [-]; 38362..38380 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	Plasmid pMAS2027 encodestwo important virulence factors: type 3 fimbriae and a type IV secretion (T4S) system
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli MS2027 [562]

Reference

[1] Ong CL et al (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl Environ Microbiol. 75(21):6783-91. [PMID:19717626]