 oriTDB accession number | 100139 |
| Name | oriT1_pMAS2027  |
Sequence Completeness  | incomplete |
| oriT length | 19 nt |
| oriT sequence | TTGCCCTATCCTGCATCGC |
| IR (inverted repeat) | _ |
| Location of nic site | [13-14] nt |
| Conserved sequence flanking the nic site | TATCCTG|C |
| Note | _ |
Visualization of oriT structure (The oriT was characterized experimentally) Reference| [1] Ong CL et al (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl Environ Microbiol. 75(21):6783-91. [PMID:19717626] |
| ID | 134 |
| Name | TaxC_pMAS2027  |
| GenBank accession number | YP_003292208 |
| Family | MOBP |
| Length | 390 aa |
| UniProt ID | D0QMQ2 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 5.40E-10, Aligned region: 66..309 |
| Note | Relaxase |
| Protein sequence [Download] | MIMGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISG GAVTRQGIRNSIDYMSRESELPVMSESGRVWTGDEILEAKDHMIDRANDPQHVMNDKGKE NKKITQNIVFSPPVSAKVKPEDLLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRI RDNDGKRADIRKKDLREIRTGFCEELKLKGYDVKATHKQQHGLNQSVKDAHNTAPKRQKG VYEVVDVGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADFGDLCSRESVKAGDLVRLK KLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLNSPDMLLKQ QQRMAQFTQQKASTLQSEQKLKTGIKFWSL |
Reference| [1] Ong CL et al (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl Environ Microbiol. 75(21):6783-91. [PMID:19717626] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 197 | Ddp1_pMAS2027 | YP_003292209 | 204 aa | similar to TaxA from R6K |
| ID | 134 |
| Name | TaxB_pMAS2027 |
| SecReT4 accesion number | _ |
| GenBank accession number | YP_003292195 |
| Family | VirD4/TraG |
| Length | 613 |
| UniProt ID | D0QMN9 |
| PDB ID | _ |
| Pfam | T4SS-DNA_transf [PF02534.13], Evalue: 1.70E-143, Aligned region: 103..580 TraG-D_C [PF12696.6], Evalue: 2.90E-24, Aligned region: 417..537 TrwB_AAD_bind [PF10412.8], Evalue: 1.70E-16, Aligned region: 309..553 |
| Note | bacterial conjugative protein involved in type IV sectretion |
| Protein sequence [Download] | MIMSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESN IRIDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEK ENDTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELW KITSKVREILLGNKVYLLDPFNSKTHQFNPLFYIDLKAESGAKDLLKLIEILFPSYGMTG AEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSIGSVVDLYSNIDRELILSK REELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYLPTVRKCTD GNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLM FLDEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIY AVSEEDDAAKISEKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEE FIILKGENPVKAEKALYYLDPYFMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEK MLSVGELESEVLL |
Reference| [1] Ong CL et al (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl Environ Microbiol. 75(21):6783-91. [PMID:19717626] |
| ID | 134 |
| Plasmid name | pMAS2027 |
| GenBank accession number | NC_013503.1 |
| Incompatibility group | IncX1 |
| Genome size | 42644 bp |
| Coordinate of oriT [Strand] | 2060..2078 [-]; 38362..38380 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | Plasmid pMAS2027 encodestwo important virulence factors: type 3 fimbriae and a type IV secretion (T4S) system |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli MS2027 [562] |
Reference| [1] Ong CL et al (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl Environ Microbiol. 75(21):6783-91. [PMID:19717626] |