oriTDB
The information of the oriT region
oriTDB accession number 100138
Name oriT_pAMbeta1  insolico
Sequence Completeness incomplete
oriT length 47 nt
oriT sequence GTCATAGAATAATTCTTTGTTTTCTTCAATCGATTCAATAGAAATAT
IR (inverted repeat)IR1: [3-10] [12-19] nt  (CATAGAAT..ATTCTTTG)
IR2: [25-31] [32-38] nt  (TTCAATC..GATTCAA)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note _
L:3-10;R:12-19;L:25-31;R:32-38
Visualization of oriT structure
Reference
[1] Kurenbach B et al (2003) Intergeneric transfer of the Enterococcus faecalis plasmid pIP501 to Escherichia coli and Streptomyces lividans and sequence analysis of its tra region. Plasmid. 50(1):86-93. [PMID:12826062]
I. Information of Relaxase
ID 133
Name ORF19_pAMbeta1 insolico
GenBank accession number YP_003305363
Family MOBQ
Length 654 aa
UniProt ID D1MAV5
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 2.40E-68, Aligned region: 11..235
Note relaxase
Protein sequence [Download] MTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDR
QTLWNKMELAEKQVNSQICREVNVALPIELNNTDQRSLIEEFVKDNFVSEGMIADVAIHR
DDENNPHAHIMLTMREVDSEGNILNKRKKIPKLDENGNPILLENGKIKMVSIKTNDWDRK
SLVSEIRKDWADKVNQYLKDRNIDQQITEKSHAELGKKELPTIHEGFYSKKLEEKGIISE
LKRKNLEIQSYNDILAELDKLESQEKVLKQDQNFTLKFEKTFSPLEKGELKNLSKELKLF
ISDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERDMLTKANEILDKQAERFF
KKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKKIFKEKPF
QTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVS
IDSVIEGEMLLAKADYYKTTDFSKVEGVARFSSEEVNSMLEQSKGFLTNIQTVKIPNDCQ
GVFFVQDSMKHIDELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDV
PEKNEVTVKMFQFAKSINRLLSGNQLQKKRNLDKLIKQTKAKKNQSLQRNIPLR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 196 _ insolico
 N/A
Information of plasmid
ID 133
Plasmid name pAMbeta1
GenBank accession number NC_013514.1
Incompatibility group Inc18
Genome size 27815 bp
Coordinate of oriT  [Strand]
Drug resistance experimental resistance to macrolide, lincosamide, streptogramin B (MLS)
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Enterococcus faecalis DS5 [565643]
Reference
[1] Kurenbach B et al (2003) Intergeneric transfer of the Enterococcus faecalis plasmid pIP501 to Escherichia coli and Streptomyces lividans and sequence analysis of its tra region. Plasmid. 50(1):86-93. [PMID:12826062]
[2] LeBlanc DJ et al (1978) Conjugal transfer of plasmid DNA among oral streptococci. Proc Natl Acad Sci U S A. 75(7):3484-7. [PMID:277950]

Microbial Bioinformatics Group in MML, SJTU