oriTDB

The information of the oriT region

oriTDB accession number	100138
Name	oriT_pAMbeta1
Sequence Completeness	incomplete
oriT length	47 nt
oriT sequence	GTCATAGAATAATTCTTTGTTTTCTTCAATCGATTCAATAGAAATAT
IR (inverted repeat)	IR1: [3-10] [12-19] nt (CATAGAAT..ATTCTTTG) IR2: [25-31] [32-38] nt (TTCAATC..GATTCAA)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	_
	L:3-10;R:12-19;L:25-31;R:32-38

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTCATAGAATAATTCTTTGTTTTCTTCAATCGATTCAATAGAAATAT

Reference

[1] Kurenbach B et al (2003) Intergeneric transfer of the Enterococcus faecalis plasmid pIP501 to Escherichia coli and Streptomyces lividans and sequence analysis of its tra region. Plasmid. 50(1):86-93. [PMID:12826062]

I. Information of Relaxase

ID	133
Name	ORF19_pAMbeta1
GenBank accession number	YP_003305363
Family	MOB_Q
Length	654 aa
UniProt ID	D1MAV5
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 2.40E-68, Aligned region: 11..235
Note	relaxase
Protein sequence [Download]	MTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDR QTLWNKMELAEKQVNSQICREVNVALPIELNNTDQRSLIEEFVKDNFVSEGMIADVAIHR DDENNPHAHIMLTMREVDSEGNILNKRKKIPKLDENGNPILLENGKIKMVSIKTNDWDRK SLVSEIRKDWADKVNQYLKDRNIDQQITEKSHAELGKKELPTIHEGFYSKKLEEKGIISE LKRKNLEIQSYNDILAELDKLESQEKVLKQDQNFTLKFEKTFSPLEKGELKNLSKELKLF ISDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERDMLTKANEILDKQAERFF KKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKKIFKEKPF QTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVS IDSVIEGEMLLAKADYYKTTDFSKVEGVARFSSEEVNSMLEQSKGFLTNIQTVKIPNDCQ GVFFVQDSMKHIDELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDV PEKNEVTVKMFQFAKSINRLLSGNQLQKKRNLDKLIKQTKAKKNQSLQRNIPLR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	196	_

N/A

Information of plasmid

ID	133
Plasmid name	pAMbeta1
GenBank accession number	NC_013514.1
Incompatibility group	Inc18
Genome size	27815 bp
Coordinate of oriT [Strand]
Drug resistance	resistance to macrolide, lincosamide, streptogramin B (MLS)
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterococcus faecalis DS5 [565643]

Reference

[1] Kurenbach B et al (2003) Intergeneric transfer of the Enterococcus faecalis plasmid pIP501 to Escherichia coli and Streptomyces lividans and sequence analysis of its tra region. Plasmid. 50(1):86-93. [PMID:12826062]

[2] LeBlanc DJ et al (1978) Conjugal transfer of plasmid DNA among oral streptococci. Proc Natl Acad Sci U S A. 75(7):3484-7. [PMID:277950]