oriTDB
The information of the oriT region
oriTDB accession number 100137
Name oriT_pGNB2  insolico
Sequence Completeness incomplete
oriT length 12 nt
oriT sequence TTACACCTTGCT
IR (inverted repeat)_
Location of nic site _
Conserved sequence flanking the
  nic site
_
Note _
D:
Visualization of oriT structure
Reference
[1] Bönemann G et al (2006) Mobilizable IncQ-related plasmid carrying a new quinolone resistance gene, qnrS2, isolated from the bacterial community of a wastewater treatment plant. Antimicrob Agents Chemother. 50(9):3075-80. [PMID:16940104]
I. Information of Relaxase
ID 132
Name ORF1_pGNB2 insolico
GenBank accession number ABE98193
Family MOBP
Length 813 aa
UniProt ID Q0GY57
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 6.40E-19, Aligned region: 8..256
Note relaxase/mobilization nuclease topoisomerase/primase fusion protein
Protein sequence [Download] MKAKVSRGGGFRGALNYVFDVGKEATHTKNAERVGGNMAGNDPRELSREFSAVRQLRPDI
GKPVWHCSLSLPPGERLSAEKWEAVTTDFMQRMGFDQTNTPWVAVRHQDTDKDHIHIVAS
RVGLDGKVWLGQWEARSAIEATQELEHTHGLTLTPGLGDARAERRKLTDKEINMAVRTGD
EPPRQRLQRLLDEAVKDKPTAPELADRLHAAGVGVRANLASTGRMNGFSFEVAGVPFKGS
DLGKGYTWAGLQKSGVTYDEARDRAGLERFRPTVADRGERQDVAAVPEPDARGLEAPTGR
SLDRDGADLGTAGPTPTGRDAGSGSLRQGDGHSAQDAGRADAADERERGEGVRAEGRGAG
QDHLRPVQQPVRAGNEHQQHEADRGAGGGLAGQSGERTEGHDASRQQTDRGSERDAPAPL
AAGAGADSGRGRGRDAGSDWASRFKQASAAKRRAADGGLGQRDLEQGHAQGARVAGTDRQ
SARELDPTAYLEASGYTVKREGRHLSVKAGGDEAYRVTRQQDGRWLWCDRYGNDGGDNID
LVREIEPGTGYAEAVYRLSGAPTVRQQPRPSEPKRQPPQLPAQGLAAREHGRDYLKGRGI
SPDTIEHAEKAGMVRYADGGVLFVGYDRTGTAQNATRRAIAPADPVQKRDLRGSDKIYPP
ILPGDPAKVWIVEGGPDALALHDIAKRSGQQPPTVIVSGGANVRSFLERDDVQAILKRAE
RVTVAGENEKNPEAQAKADAGHQKQAQRVAEITGREVRQWTPKPEHGKDLADMNARQVAE
IERKRQAEIEAERARNRELSRKSRGYDGPSFGR
Reference
[1] Bönemann G et al (2006) Mobilizable IncQ-related plasmid carrying a new quinolone resistance gene, qnrS2, isolated from the bacterial community of a wastewater treatment plant. Antimicrob Agents Chemother. 50(9):3075-80. [PMID:16940104]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 195 MobC_pGNB2 insolico ABE98194 143 aa auxiliary mobilization protein C
 N/A
Information of plasmid
ID 132
Plasmid name pGNB2
GenBank accession number DQ460733.1
Incompatibility group IncQ
Genome size 8469 bp
Coordinate of oriT  [Strand]
Drug resistance experimental quinolone resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Uncultured bacterium [77133]
Reference
[1] Bönemann G et al (2006) Mobilizable IncQ-related plasmid carrying a new quinolone resistance gene, qnrS2, isolated from the bacterial community of a wastewater treatment plant. Antimicrob Agents Chemother. 50(9):3075-80. [PMID:16940104]