oriTDB

The information of the oriT region

oriTDB accession number	100136
Name	oriT_pLAC1
Sequence Completeness	incomplete
oriT length	32 nt
oriT sequence	AACATAAAGTATAGTGAGTTATACTTTACTTG
IR (inverted repeat)	[5-13] [20-28] nt (TAAAGTATA..TATACTTTA)
Location of nic site	[17-18] nt
Conserved sequence flanking the nic site	TAGTGA\|GTTAT
Note	pMV158 superfamily; nick site (TGA′G)
	L:5-13;R:20-28;D:17-18

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AACATAAAGTATAGTGAGTTATACTTTACTTG

Reference

[1] Asteri IA et al (2010) Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector. Int J Food Microbiol. 141(3):222-8. [PMID:20538362]

I. Information of Relaxase

ID	131
Name	Mob_pLAC1
GenBank accession number	YP_003650630
Family	MOB_V
Length	361 aa
UniProt ID	D6CHH5
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 1.20E-77, Aligned region: 1..194
Note	clade A of the pMV158-superfamily of relaxases
Protein sequence [Download]	MSFAVARMTKLKADNLVGIGNHDQRKTTNHSNEDIDVSRSHLNYDLVAGRTDNFKTDIEA YINENKASKRAVRKDAVLVNEWIITSDKDFFEQLDEAETRKYFETAKQYFADNYGDENIR YAVVHMDEKTPHMHMGIVPFDDGKKLSAKRIFNREALQRIQEELPQYLKENGFDVQRGNK NKERKNLSVPEYKAMREDLKKIETEKQETQAKLADTKKQLDRIKPRDTKKIASKPTLMNK NKVTVDKSDLADLEQRAVTSDAYNFEKIHLEVENHSLRNDLGEAKGQNYELKKENERLQK LVGTLQGIIRNVDGFLHKKLGINLPEKWLERAGLKEPSKKAPESSQELGKHKSDELGGPH L

Reference

[1] Asteri IA et al (2010) Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector. Int J Food Microbiol. 141(3):222-8. [PMID:20538362]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	194	_

N/A

Information of plasmid

ID	131
Plasmid name	pLAC1
GenBank accession number	NC_014164.1
Genome size	3478 bp
Coordinate of oriT [Strand]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactobacillus acidipiscis ACA-DC 1533 [89059]

Reference
N/A