oriTDB

The information of the oriT region

oriTDB accession number	100135
Name	oriT_pPAGA3
Sequence Completeness	intact
oriT length	246 nt
oriT sequence	GATGCTCGGTGACCTGTCGGGTAACTCTGACATGGCCATCAGTGACCCGCTGTCTGGAAA TCTCAATGACTTCTTCCGCCAGTCTTATTACCTGGTCGTTACCCTCTGACCCTGGTTCTT CAGGAGGTTGATTGTTCATGGTTAACCTGCCTGTAATACAGAGCGATCATTTGTACTTTT CCTGACCTTGAGCATAAGTGACTGCTTTCCGGCTGATTAAAGCCTGGCACAAAGAACTAC AAAAGA
IR (inverted repeat)	IR1: [151-155] [157-161] nt (CTGTA..TACAG) IR2: [204-208] [219-223] nt (GCTTT..AAAGC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	_
	L:151-155;R:157-161;L:204-208;R:219-223

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GATGCTCGGTGACCTGTCGGGTAACTCTGACATGGCCATCAGTGACCCGCTGTCTGGAAA

61

TCTCAATGACTTCTTCCGCCAGTCTTATTACCTGGTCGTTACCCTCTGACCCTGGTTCTT

121

CAGGAGGTTGATTGTTCATGGTTAACCTGCCTGTAATACAGAGCGATCATTTGTACTTTT

181

CCTGACCTTGAGCATAAGTGACTGCTTTCCGGCTGATTAAAGCCTGGCACAAAGAACTAC

241

AAAAGA

Reference
N/A

N/A

Information of plasmid

ID	130
Plasmid name	pPAGA3
GenBank accession number	NC_014227.1
Genome size	2734 bp
Coordinate of oriT [Strand]	125..370 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Pantoea agglomerans EGE6 [549]

Reference

[1] de Lima Procópio RE et al (2011) Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector. Genet Mol Biol. 34(1):103-9. [PMID:21637551]