 oriTDB accession number | 100134 |
| Name | oriT_KOPRI126573  |
Sequence Completeness  | intact |
| oriT length | 430 nt |
| oriT sequence | TTTGCACCCTATCTGCACCCCTATTGCACTCTATGTGCATCTTACTCGCCCCCTACCTGT AGTCAAGTTGCACTCTTCCTGCTCTCTTCTAGCACCCTTTCTGCATACCATCTGTGCTAC CCCCCTACGGCCTTCGGCCTGGCTAGTGGATACGCCCTTAACAAGAGCTTCAACTTTTCC CCTTAACAGGGTCAAAGTCTCAATTCAAAGTCTCAATACACAATACGCACCCCCCTAGCG GGAGGTGACAGCCGCGCTGTGCGCGGCCTGCAGAGAAAAAGTGGTGTTTTTATGCGCTAT ACATATGTATTACAAAAGTACAGTCTAGATCAGCAAAATCAAGCTTTTTGTGTATTACAT TTGCTATACACTGTTTCGGCAGTTATCGTTAGCCTGAATATGTCCCATTTTGGGCTCAGA GAGGAACACC |
| IR (inverted repeat) | IR1: [276-280] [287-291]nt (AAAAA..TTTTT) [299-304] [305-310] nt (ATACAT..ATGTAT) [335-340] [343-348] nt (AAAATC..GCTTTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site | _ |
| Note | |
Visualization of oriT structure Reference| [1] Dziewit L et al (2014) Plasmids of psychrophilic and psychrotolerant bacteria and their role in adaptation to cold environments. Front Microbiol. 0.622222222. [PMID:25426110] |
| ID | 129 |
| Name | TraC_KOPRI126573 |
| GenBank accession number | YP_005083667 |
| Family | MOBF |
| Length | 978 aa |
| UniProt ID | G9G7H8 |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 5.60E-82, Aligned region: 9..287 AAA_30 [PF13604.5], Evalue: 3.00E-40, Aligned region: 473..672 Viral_helicase1 [PF01443.17], Evalue: 1.50E-05, Aligned region: 826..915 AAA_19 [PF13245.5], Evalue: 8.70E-07, Aligned region: 478..614 UvrD_C_2 [PF13538.5], Evalue: 1.10E-06, Aligned region: 867..914 |
| Note | TrwC relaxase; pfam08751 |
| Protein sequence [Download] | MFNVTSIKGSNQYAAAGYYTAADDYYAKESPGEWQGIGAELLGLAGPIDQKELAKLFDGK LPNGEVMPKTFDKETGNRRMGLDLTFSAPKSVSMQALIAGDKDVVAAHDRAVTKAMEHVE KLAQARRKEHGKSMLERTGNLIIGKFRHELSRAKDPQLHTHAVVMNATRREDGKWRAIHN DDIFKIQPQIDAMYKGELAKELRELGYEIRVLDKDGNFELAHISRDQIEAFSSRAKVIEE ALAKDGKTRSNATALEKQIIAMATRPRKDERDRHLVKEYWVTKARDLGIEFGGRSQLDNQ EYGRRSESIHAEHNLPEGITAGQAVVQYAINHLTEREQVVGESDLRTAALRRAVGLASPS EVDDEIKRLVKQGTLIESPPTYRMATGKDGAALSPAGWRALLKEQKGWSEKEAQQYVKMA INRGSLVEAEKRYTTQRALKREKAILAIERSGRGQVAPLLSKEQVAKALESSTLSAGQYQ AVEVIVSTSNRFVGIQGDAGTGKTYSVDRAVKLIESVNNAMTERSTTTDAVFRVVALAPY GNQVTALKNEGLDAHTLASFFHTKDKKLDERTIVVLDEAGVVGARQMEQLMRIIEQSGAR LVQLGDTKQTEAIEAGKPFAQLQQNGMQTARIKEIQRQKNQELKIAVQHAADGNPGKSLE HVNHVEELREPGQRHQAIVRDYMSLTPQERKEVLIVAGTNKDRKQINAMTRESLGLVGNG KELPTLNRVDTTQAERRYAPSYKKGMIVQPEKDYIRAGLSRGELYTVDQALPGNVLVVKD KNGNRVEFNPRKLTKLSVYNLEKPEFSVGDLVRITRNDQKLDLTNGDRMRVVGNANGVIE LASLKEKDGTPERVVALPTNRPLHLEHAYSATVHSAQGLTNDRVMISINTKSLTTSQNLW YVAISRARHEARIYTDSIAGLPAAIANRYDKTTALSLQQARERQRNESIKPRTVLDGKEL ERKQRSALDGAGLGKSGV |
Reference| [1] Dziewit L et al (2014) Plasmids of psychrophilic and psychrotolerant bacteria and their role in adaptation to cold environments. Front Microbiol. 0.622222222. [PMID:25426110] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 192 | _ |
| ID | 129 |
| Name | TraB_KOPRI126573 |
| SecReT4 accesion number | _ |
| GenBank accession number | YP_005083666 |
| Family | TrwB/TraD |
| Length | 519 |
| UniProt ID | G9G7H7 |
| PDB ID | _ |
| Pfam | TrwB_AAD_bind [PF10412.8], Evalue: 1.10E-103, Aligned region: 124..500 TraG-D_C [PF12696.6], Evalue: 4.90E-21, Aligned region: 363..481 T4SS-DNA_transf [PF02534.13], Evalue: 3.70E-15, Aligned region: 240..512 |
| Note | Type IV secretion-system coupling protein DNA-binding domain; pfam10412 |
| Protein sequence [Download] | MHKREVDSAVMTAGFGLPIAGWAAGVYFAQMPLTPFPAAFKETLHNGWHNPIVLGATIAT SAVAASLVYYLYEYCDDGFRGEQYQEFLRGSEIKNWHTVKAKVRRRNEKTNRERKKWGLA KSEPIMIGKLPMPLHLEDRNTMICASIGAGKSVTMEGMIASALKRGDRLAVVDPNGTFYS KFSFKGDSILNPFDERSVGWTIFNEIRGIHDFNRMAKSIIPPQVDPSDEQWCAYARDVLS DTMRKLKETNNPDQDTLVNLLVREDGDTIRAFLANTDSEGYFRDNAEKAIASIQFMMNKY IRPLRYMSKGEFSIYKWVNDPNAGNLFITWREDMRDTMKPLVATWIDTICATILSSEPMT GKRLWLFLDELQSLGKLESFVPAATKGRKHGLRMVGSLQDWSQLNASYGRDDAETLLSCF RNYVILAAANAKNAGMASEILGSHDVKRWRKSYTAGKLTRTEEVTRDEPVVQASEISNLP DLVAYVKFGEDFPITKVKTPYIDYKERAQAIMLTGQAAA |
Reference| ID | 129 |
| Plasmid name | KOPRI126573 |
| GenBank accession number | NC_016644.1 |
| Genome size | 81814 bp |
| Coordinate of oriT [Strand] | |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Pseudomonas sp. MC1 [1123041] |
Reference