oriTDB

The information of the oriT region

oriTDB accession number	100133
Name	oriT_pGL3
Sequence Completeness	intact
oriT length	233 nt
oriT sequence	CTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTA TAAAATGGGTAGGTGGCGTTTGCCTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGG GGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGTTTTGTA TTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTAT
IR (inverted repeat)	IR1: [46-55] [60-69] nt (CCCATTTTAT..ATAAAATGGG) IR2: [115-127] [131-143] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [150-163] [176-189] nt (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site	[198-199] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	98% DNA identical to oriT of the lactococcal plasmids pCD4, pSRQ800, pSRQ900 and pIL7
	L:46-55;R:60-69;L:115-127;R:131-143;L:150-163;R:176-189;D:198-199

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTA

61

TAAAATGGGTAGGTGGCGTTTGCCTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGG

121

GGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGTTTTGTA

181

TTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTAT

Reference

[1] Aguado-Urda M et al (2012) Characterization of plasmids in a human clinical strain of Lactococcus garvieae. PLoS One. 7(6):e40119. [PMID:22768237]

N/A

Information of plasmid

ID	128
Plasmid name	pGL3
GenBank accession number	NC_016970.1
Genome size	12948 bp
Coordinate of oriT [Strand]	65..297 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus garvieae 21881 [1363]

Reference
N/A