oriTDB

The information of the oriT region

oriTDB accession number	100132
Name	oriT_pE001
Sequence Completeness	incomplete
oriT length	26 nt
oriT sequence	GGCGTTTGCCCTATCCTGCATCACAG
IR (inverted repeat)	_
Location of nic site	[18-19] nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note	putative oriT of IncX1 plasmids
	D:19-20

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGCGTTTGCCCTATCCTGCATCACAG

Reference
N/A

I. Information of Relaxase

ID	127
Name	TaxC_pE001
GenBank accession number	YP_006953019
Family	MOB_P
Length	388 aa
UniProt ID	G1CCM2
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 6.30E-10, Aligned region: 64..306
Note	relaxase
Protein sequence [Download]	MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGA ITRQGIRNSIDYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENK KITQNIVFSPPVSAKVKPEDLLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRD NDGKRADIRKKDLREIRTGFCEELKLKGYDVKATHKQQHGLNQSVKDAHNTAPKRQKGVY EVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADFGDLCSRESVKAGDLVRLKKL GQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLNSPDMLLKQQQ RMAQFTQQKASTLQSEQKLKTGIKFLGL

Reference

[1] Bielak E et al (2011) Investigation of diversity of plasmids carrying the bla_TEM-52 gene. J Antimicrob Chemother. 66(11):2465-74. [PMID:21831988]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	190	TaxA_pE001	YP_006953020	204 aa	_

Information of T4CP

ID	127
Name	TaxB_pE001
SecReT4 accesion number	_
GenBank accession number	YP_006953056
Family	VirD4/TraG
Length	611
UniProt ID	G1CCQ9
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 1.40E-142, Aligned region: 101..578 TraG-D_C [PF12696.6], Evalue: 3.50E-24, Aligned region: 415..535 TrwB_AAD_bind [PF10412.8], Evalue: 2.70E-16, Aligned region: 307..551
Note	Type IV Secretion System Coupling Protein
Protein sequence [Download]	MSLKLPDKGQWVFIGLVMCLVTYYAGSVSVYFLNGKTPLYIWKNFDSMLLWKIITESNIR TDIRLTAIPSLLSGMVSSLIVPVFVIWQLNKMDVALYGDAKFASDNDLKKSKLLKWETEN DTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKI TSKVREILLGNKVYLLDPFNSKTHQFNPLFYIDLKAESGAKDLLKLIEILFPSYGMTGAE AHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSIGSVVDLYSNIDRELILSKRE ELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYLPTVRKCTDGN DFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAV SEEDDAAKISEKLGYITTTSKSTSKSRGRSASQGESESEARRALVLPQELGTLDFKEEFI ILKGENPVKAEKALYYLDPYFMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKML SVGELESEVLL

Reference
N/A

Information of plasmid

ID	127
Plasmid name	pE001
GenBank accession number	NC_019067.1
Incompatibility group	IncX1
Genome size	38611 bp
Coordinate of oriT [Strand]	7136..7161 [-]
Drug resistance	carrying the bla_TEM-52 gene
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli 2161 [562]

Reference

[1] Bielak E et al (2011) Investigation of diversity of plasmids carrying the bla_TEM-52 gene. J Antimicrob Chemother. 66(11):2465-74. [PMID:21831988]