 oriTDB accession number | 100132 |
| Name | oriT_pE001  |
Sequence Completeness  | incomplete |
| oriT length | 26 nt |
| oriT sequence | GGCGTTTGCCCTATCCTGCATCACAG |
| IR (inverted repeat) | _ |
| Location of nic site | [18-19] nt |
| Conserved sequence flanking the nic site | TATCCTG|C |
| Note | putative oriT of IncX1 plasmids |
Visualization of oriT structure Reference| ID | 127 |
| Name | TaxC_pE001 |
| GenBank accession number | YP_006953019 |
| Family | MOBP |
| Length | 388 aa |
| UniProt ID | G1CCM2 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 6.30E-10, Aligned region: 64..306 |
| Note | relaxase |
| Protein sequence [Download] | MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGA ITRQGIRNSIDYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENK KITQNIVFSPPVSAKVKPEDLLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRD NDGKRADIRKKDLREIRTGFCEELKLKGYDVKATHKQQHGLNQSVKDAHNTAPKRQKGVY EVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADFGDLCSRESVKAGDLVRLKKL GQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLNSPDMLLKQQQ RMAQFTQQKASTLQSEQKLKTGIKFLGL |
Reference| [1] Bielak E et al (2011) Investigation of diversity of plasmids carrying the blaTEM-52 gene. J Antimicrob Chemother. 66(11):2465-74. [PMID:21831988] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 190 | TaxA_pE001 | YP_006953020 | 204 aa | _ |
| ID | 127 |
| Name | TaxB_pE001 |
| SecReT4 accesion number | _ |
| GenBank accession number | YP_006953056 |
| Family | VirD4/TraG |
| Length | 611 |
| UniProt ID | G1CCQ9 |
| PDB ID | _ |
| Pfam | T4SS-DNA_transf [PF02534.13], Evalue: 1.40E-142, Aligned region: 101..578 TraG-D_C [PF12696.6], Evalue: 3.50E-24, Aligned region: 415..535 TrwB_AAD_bind [PF10412.8], Evalue: 2.70E-16, Aligned region: 307..551 |
| Note | Type IV Secretion System Coupling Protein |
| Protein sequence [Download] | MSLKLPDKGQWVFIGLVMCLVTYYAGSVSVYFLNGKTPLYIWKNFDSMLLWKIITESNIR TDIRLTAIPSLLSGMVSSLIVPVFVIWQLNKMDVALYGDAKFASDNDLKKSKLLKWETEN DTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKI TSKVREILLGNKVYLLDPFNSKTHQFNPLFYIDLKAESGAKDLLKLIEILFPSYGMTGAE AHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSIGSVVDLYSNIDRELILSKRE ELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYLPTVRKCTDGN DFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAV SEEDDAAKISEKLGYITTTSKSTSKSRGRSASQGESESEARRALVLPQELGTLDFKEEFI ILKGENPVKAEKALYYLDPYFMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKML SVGELESEVLL |
Reference| ID | 127 |
| Plasmid name | pE001 |
| GenBank accession number | NC_019067.1 |
| Incompatibility group | IncX1 |
| Genome size | 38611 bp |
| Coordinate of oriT [Strand] | 7136..7161 [-] |
| Drug resistance | carrying the blaTEM-52 gene |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli 2161 [562] |
Reference| [1] Bielak E et al (2011) Investigation of diversity of plasmids carrying the blaTEM-52 gene. J Antimicrob Chemother. 66(11):2465-74. [PMID:21831988] |