oriTDB

The information of the oriT region

oriTDB accession number	100130
Name	oriT_pEC34B
Sequence Completeness	intact
oriT length	88 nt
oriT sequence	TCAGGGGGTGTCGGGGCGAAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTG GCTTAACTATGTGGCATCAGTGCGGATT
IR (inverted repeat)	[8-17] [21-30] nt (GTGTCGGGGC..GCCCTGACCC)
Location of nic site	[61-62] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	ColE1 superfamily
	L:8-17;R:21-30;D:61-62

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCAGGGGGTGTCGGGGCGAAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTG

61

GCTTAACTATGTGGCATCAGTGCGGATT

Reference
N/A

I. Information of Relaxase

ID	125
Name	MobA_pEC34B
GenBank accession number	YP_006953583
Family	MOB_P
Length	517 aa
UniProt ID	G9BMJ6
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 9.60E-20, Aligned region: 41..242
Note	relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAESVTRGIDA LYHVGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGV REKAERESRIYRDNAERRVQQARKICKQGCDLKRDENQRRYSPVHNVDRGIAGKTPGRGE RGDDAAQEGRVKAGREYGHEVAGDSLSPVYREWRDALVSWRADTGEPGRNQDTGRNIAET EREDMGRGVCAGREKEISGPSVREIGNGDSLSGEGLGTTDGVIQSDGAGKTFAERIRATA TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQ EIRDERLIQEREHALSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	187	MobB_pEC34B	YP_006953585	172 aa	plasmid mobilization protein
2	188	MobC_pEC34B	YP_006953586	115 aa	plasmid mobilization protein

N/A

Information of plasmid

ID	125
Plasmid name	pEC34B
GenBank accession number	NC_019078.1
Genome size	6952 bp
Coordinate of oriT [Strand]	2543..2630 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli NCTC 9034 [562]

Reference
N/A