oriTDB
The information of the oriT region
oriTDB accession number 100130
Name oriT_pEC34B  insolico
Sequence Completeness intact
oriT length 88 nt
oriT sequence TCAGGGGGTGTCGGGGCGAAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTG GCTTAACTATGTGGCATCAGTGCGGATT
IR (inverted repeat)[8-17] [21-30] nt  (GTGTCGGGGC..GCCCTGACCC)
Location of nic site [61-62] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note ColE1 superfamily
L:8-17;R:21-30;D:61-62
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 125
Name MobA_pEC34B insolico
GenBank accession number YP_006953583
Family MOBP
Length 517 aa
UniProt ID G9BMJ6
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 9.60E-20, Aligned region: 41..242
Note relaxase
Protein sequence [Download] MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF
AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRL
QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAESVTRGIDA
LYHVGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGV
REKAERESRIYRDNAERRVQQARKICKQGCDLKRDENQRRYSPVHNVDRGIAGKTPGRGE
RGDDAAQEGRVKAGREYGHEVAGDSLSPVYREWRDALVSWRADTGEPGRNQDTGRNIAET
EREDMGRGVCAGREKEISGPSVREIGNGDSLSGEGLGTTDGVIQSDGAGKTFAERIRATA
TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQ
EIRDERLIQEREHALSLERERQPEIQERTLDGPSLGW
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 187 MobB_pEC34B insolico YP_006953585 172 aa plasmid mobilization protein
2 188 MobC_pEC34B insolico YP_006953586 115 aa plasmid mobilization protein
 N/A
Information of plasmid
ID 125
Plasmid name pEC34B
GenBank accession number NC_019078.1
Genome size 6952 bp
Coordinate of oriT  [Strand] 2543..2630 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli NCTC 9034 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU