oriTDB
The information of the oriT region
oriTDB accession number 100128
Name oriT_pHHV216  insolico
Sequence Completeness intact
oriT length 394 nt
oriT sequence TCAAGCTGAAGCGACTGAAGAACTTGAAGTTAAAAAACTGATGGATCAATCGAACCAGAA AGAGCAGCCAGAAGCTAAAGAAGAAGATCCAGACAAAATCAAAAAACCGCTTTTAGTTGT TATTCATGACGGCAGACAAGCGAACCTGATGCTTAATAAAAAGCCTTCTTCTTTGGGTTT TGCATGGATCAAATACGAAGATGGAACAGAGGTATCCGTAGATTGCAGCAGCCTTGAAAT CGAGTATTTACAAGAAGCCTAAACCTACCAATGGAAAAAGACCTTGCTAATGCAAGGTCT TTTTTTGGGCGGGTTTTCCCGACCACCTAGCTTGATTACCCTTAGTCATGACTAAGGAGA AAAGAGGGAAAAAAAGAAGATATATAATCTAGGG
IR (inverted repeat)[275-287] [292-304] nt  (AAAAAGACCTTGC..GCAAGGTCTTTTT)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note _
L: 275-287;R:292-304
Visualization of oriT structure
Reference
[1] Heuer H et al (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content. Environ Microbiol. 11(4):937-49. [PMID:19055690]
I. Information of Relaxase
ID 123
Name MobC_pHHV216 insolico
GenBank accession number YP_006960697
Family Other
Length 223 aa
UniProt ID B5TTF6
PDB ID _
Pfam MobC [PF05713.10], Evalue: 2.10E-05, Aligned region: 168..211
Note relaxase
Protein sequence [Download] MELIMTKDNKIFFRVDRAVLDWWEAKCEKQGIKKGAPFQKALELSYQKENDPEYSGQDFS
LNLEKDLAEKIYHLAKFEEVDPDDLIKKMIKEKLSEIEKTQDVIDVFEKTSRKELRLKPS
LMKAINEKAAQIGMKPNQYIISVLTANVTKDSIFFSQEEALKLGESNSQLLAIGRNLNQM
AKNMNQGIYESYDREFVERVHQLVKAQVRHVFTLLERNKKRWE
Reference
[1] Heuer H et al (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content. Environ Microbiol. 11(4):937-49. [PMID:19055690]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 185 _ insolico
Information of T4CP
ID 123
Name TraN_pHHV216 insolico
SecReT4 accesion number _
GenBank accession number YP_006960676
Family VirD4/TraG
Length 707
UniProt ID B5TTD5
PDB ID _
Pfam T4SS-DNA_transf [PF02534.13], Evalue: 5.80E-99, Aligned region: 2..447
TraG-D_C [PF12696.6], Evalue: 1.70E-22, Aligned region: 282..405
TrwB_AAD_bind [PF10412.8], Evalue: 3.30E-14, Aligned region: 21..420
Note coupling of DNA transfer and mating pair formation
Protein sequence [Download] MGNKGILVGKYHNKYLMLGGQQFVLLAAPTRSGKGVAIVIPNLLNYSDSVVVLDLKLENF
LLTSKFRAKNGQKVYLFSPFSEDLKSHKWNPFDTVSHDENFRVGEILAIGRSFYPVTGDA
KTDFWNDNANNLFLGLALYLFETEDLPVTMGEVLRQSSGKGKPIQDHIRSIIDERAQSDK
PLSDTCLDALNRFLSSPDNTLGNIISTFTSPLTIFANPIVDAATSSSDFKLEDVRRQRMS
IYFGVQPNRLGDAKNLINLFYSLLIDVNLKTLPQQDSSLKYQCLVVNDEATAVGKVAKIA
DANSFIAGYNIRLLTIIQSIGQLEDVYKTQTRGLVTNHALQIVYPPREQRDANEYSEMLG
YFTFKAKSKGQSRSMAWGQGGSNSENESDQRRALMLPQELKELSQDKEIIFMENTKPIMC
EKAKYFNDSAFVDRLKSVSPSMKAIKGFPTHKQLEKIAFEDLELSIPIPDLDVKLHKAKI
EKRIAAITTVEQAEQIDISSLVIPEFQLDKTVFEDPENPQPEECLQVVDQFFAAIEWEED
TEAQNIVDDFFSQVEASAENPVESLVAVGASEQAENLVAADDSDPLAMIAEMKNLIAVDE
PEQAENLVAADESDPLAMIAEMENLIAVDEPEQLENLISVDDTEFSYDEMDAEQIVDTFF
NCAESEFSIDEDLQDNPVAADEGQNGSIENSDLDDVYQAFSNQVNSK

Reference
[1] Heuer H et al (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content. Environ Microbiol. 11(4):937-49. [PMID:19055690]
Information of plasmid
ID 123
Plasmid name pHHV216
GenBank accession number NC_019217.1
Genome size 58274 bp
Coordinate of oriT  [Strand]
Drug resistance experimental resistance to Sulfadiazine (SDZ), streptomycin, tetracycline, chloramphenicol
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Uncultured bacterium HHV216 [558687]
Reference
[1] Heuer H et al (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content. Environ Microbiol. 11(4):937-49. [PMID:19055690]

Microbial Bioinformatics Group in MML, SJTU