oriTDB

The information of the oriT region

oriTDB accession number	100128
Name	oriT_pHHV216
Sequence Completeness	intact
oriT length	394 nt
oriT sequence	TCAAGCTGAAGCGACTGAAGAACTTGAAGTTAAAAAACTGATGGATCAATCGAACCAGAA AGAGCAGCCAGAAGCTAAAGAAGAAGATCCAGACAAAATCAAAAAACCGCTTTTAGTTGT TATTCATGACGGCAGACAAGCGAACCTGATGCTTAATAAAAAGCCTTCTTCTTTGGGTTT TGCATGGATCAAATACGAAGATGGAACAGAGGTATCCGTAGATTGCAGCAGCCTTGAAAT CGAGTATTTACAAGAAGCCTAAACCTACCAATGGAAAAAGACCTTGCTAATGCAAGGTCT TTTTTTGGGCGGGTTTTCCCGACCACCTAGCTTGATTACCCTTAGTCATGACTAAGGAGA AAAGAGGGAAAAAAAGAAGATATATAATCTAGGG
IR (inverted repeat)	[275-287] [292-304] nt (AAAAAGACCTTGC..GCAAGGTCTTTTT)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	_
	L: 275-287;R:292-304

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCAAGCTGAAGCGACTGAAGAACTTGAAGTTAAAAAACTGATGGATCAATCGAACCAGAA

61

AGAGCAGCCAGAAGCTAAAGAAGAAGATCCAGACAAAATCAAAAAACCGCTTTTAGTTGT

121

TATTCATGACGGCAGACAAGCGAACCTGATGCTTAATAAAAAGCCTTCTTCTTTGGGTTT

181

TGCATGGATCAAATACGAAGATGGAACAGAGGTATCCGTAGATTGCAGCAGCCTTGAAAT

241

CGAGTATTTACAAGAAGCCTAAACCTACCAATGGAAAAAGACCTTGCTAATGCAAGGTCT

301

TTTTTTGGGCGGGTTTTCCCGACCACCTAGCTTGATTACCCTTAGTCATGACTAAGGAGA

361

AAAGAGGGAAAAAAAGAAGATATATAATCTAGGG

Reference

[1] Heuer H et al (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content. Environ Microbiol. 11(4):937-49. [PMID:19055690]

I. Information of Relaxase

ID	123
Name	MobC_pHHV216
GenBank accession number	YP_006960697
Family	Other
Length	223 aa
UniProt ID	B5TTF6
PDB ID	_
Pfam	MobC [PF05713.10], Evalue: 2.10E-05, Aligned region: 168..211
Note	relaxase
Protein sequence [Download]	MELIMTKDNKIFFRVDRAVLDWWEAKCEKQGIKKGAPFQKALELSYQKENDPEYSGQDFS LNLEKDLAEKIYHLAKFEEVDPDDLIKKMIKEKLSEIEKTQDVIDVFEKTSRKELRLKPS LMKAINEKAAQIGMKPNQYIISVLTANVTKDSIFFSQEEALKLGESNSQLLAIGRNLNQM AKNMNQGIYESYDREFVERVHQLVKAQVRHVFTLLERNKKRWE

Reference

[1] Heuer H et al (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content. Environ Microbiol. 11(4):937-49. [PMID:19055690]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	185	_

Information of T4CP

ID	123
Name	TraN_pHHV216
SecReT4 accesion number	_
GenBank accession number	YP_006960676
Family	VirD4/TraG
Length	707
UniProt ID	B5TTD5
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 5.80E-99, Aligned region: 2..447 TraG-D_C [PF12696.6], Evalue: 1.70E-22, Aligned region: 282..405 TrwB_AAD_bind [PF10412.8], Evalue: 3.30E-14, Aligned region: 21..420
Note	coupling of DNA transfer and mating pair formation
Protein sequence [Download]	MGNKGILVGKYHNKYLMLGGQQFVLLAAPTRSGKGVAIVIPNLLNYSDSVVVLDLKLENF LLTSKFRAKNGQKVYLFSPFSEDLKSHKWNPFDTVSHDENFRVGEILAIGRSFYPVTGDA KTDFWNDNANNLFLGLALYLFETEDLPVTMGEVLRQSSGKGKPIQDHIRSIIDERAQSDK PLSDTCLDALNRFLSSPDNTLGNIISTFTSPLTIFANPIVDAATSSSDFKLEDVRRQRMS IYFGVQPNRLGDAKNLINLFYSLLIDVNLKTLPQQDSSLKYQCLVVNDEATAVGKVAKIA DANSFIAGYNIRLLTIIQSIGQLEDVYKTQTRGLVTNHALQIVYPPREQRDANEYSEMLG YFTFKAKSKGQSRSMAWGQGGSNSENESDQRRALMLPQELKELSQDKEIIFMENTKPIMC EKAKYFNDSAFVDRLKSVSPSMKAIKGFPTHKQLEKIAFEDLELSIPIPDLDVKLHKAKI EKRIAAITTVEQAEQIDISSLVIPEFQLDKTVFEDPENPQPEECLQVVDQFFAAIEWEED TEAQNIVDDFFSQVEASAENPVESLVAVGASEQAENLVAADDSDPLAMIAEMKNLIAVDE PEQAENLVAADESDPLAMIAEMENLIAVDEPEQLENLISVDDTEFSYDEMDAEQIVDTFF NCAESEFSIDEDLQDNPVAADEGQNGSIENSDLDDVYQAFSNQVNSK

Reference

[1] Heuer H et al (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content. Environ Microbiol. 11(4):937-49. [PMID:19055690]

Information of plasmid

ID	123
Plasmid name	pHHV216
GenBank accession number	NC_019217.1
Genome size	58274 bp
Coordinate of oriT [Strand]
Drug resistance	resistance to Sulfadiazine (SDZ), streptomycin, tetracycline, chloramphenicol
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Uncultured bacterium HHV216 [558687]

Reference

[1] Heuer H et al (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content. Environ Microbiol. 11(4):937-49. [PMID:19055690]