oriTDB
The information of the oriT region
oriTDB accession number 100127
Name oriT_pLJ42  insolico
Sequence Completeness intact
oriT length 310 nt
oriT sequence AAAAGCCGATTTGCGAACAGCAAACTTTCGGCTTTTTTTGTGCGGTTTTTTTGTAACCTT TTTTTGCAAAAAAAGGGCAGGTTTTTGGGTTTGAACTTGTGTCGTTTTGCGAAGCGAAAT CGGCATGCTTTTGACTTTGATTTTAGGGGGATAAAAGGGGGAGCTAAGCGCCCTCTTTTC AAGCGGGAATGGGCATACTTTTGCGAAGCAAAATGTATGTCATTCCGAAAGCTTGCAGTA TTTGTTTTTTAATGTTAGGGTAAACGTGACCGAAAAGGAAGCGTTTATACTTACATTAAC TCATTAAAAA
IR (inverted repeat)IR1: [1-9] [28-36] nt  (AAAAGCCGA..TCGGCTTTT)
IR2: [57-66] [67-76] nt  (TGTCCTTTTTTTG..CAAAAAAAGGGCA)
IR3: [153-164] [168-179] nt  (AAAAGGGGGAGC..GCGCCCTCTTTT)
IR4: [199-204] [208-213] nt  (TTTTGC..GCAAAA)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note _
L:1-9;R:28-36;L:57-66;R:67-76;L:153-164;R:168-179;L:199-204;R:208-213
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 122
Name MobA_pLJ42 insolico
GenBank accession number YP_006960783
Family MOBP
Length 445 aa
UniProt ID Q4F8M8
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 5.30E-62, Aligned region: 9..280
Note relaxase
Protein sequence [Download] MATIAKITNGASAASALNYALGKDKELHNDTKQWLEDNRLERPPELTNCRAVAVGGTNGI
DISIANEQFKIVQQAFNQTARRNQVLRITQSFALNELDPTNTRDWQRANDLGCELAEKLY
PDYQTAVYTHLDGQNHILHNHIIINKVNLQTGKKLDERKGSAVERARNANDEISKEQGWK
VIEPVREHQSRTEQDLTKKGQYSYMADLRGRIDTTMQDTSICDFKTFSDVLGQSGVIVRE
RGQSFSYAFLDANNKQRRARGKRLGTDYDKEAIDNELARRTRERTEQQLDREDQARSTEL
VRDTTATDTALEQRERDTEPRKPALNQQSTKIERLGTSMQEINDRGSHAEQIYQRIRPAV
RTIADGLQRFTDAVHGFAKQIKQRLADQHLYQDVASRFKADMARKEQAQHKEVKQDLAKR
TAPKPQRAPERSTQTYYHDRGGLER
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 183 MobB_pLJ42 insolico YP_006960784 229 aa mobilization protein
2 184 MobC_pLJ42 insolico YP_006960782 112 aa mobilization protein
 N/A
Information of plasmid
ID 122
Plasmid name pLJ42
GenBank accession number NC_019219.1
Genome size 5529 bp
Coordinate of oriT  [Strand]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactobacillus plantarum [1590]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU