oriTDB

The information of the oriT region

oriTDB accession number	100124
Name	oriT_pMBLT00
Sequence Completeness	incomplete
oriT length	20 nt
oriT sequence	CTCGAAAAAGGTTTTACGAG
IR (inverted repeat)	[1-9] [12-20] nt (CTCGTAAAA..TTTTTCGAG)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note
	L:1-9;R:12-20

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTCGAAAAAGGTTTTACGAG

Reference

[1] Chae HS et al (2013) Use of a novel Escherichia coli-leuconostoc shuttle vector for metabolic engineering of Leuconostoc citreum to overproduce D-lactate. Appl Environ Microbiol. 79(5):1428-35. [PMID:23241984]

I. Information of Relaxase

ID	119
Name	MobA_pMBLT00
GenBank accession number	AFJ91158
Family	MOB_V
Length	309 aa
UniProt ID	I6Q9D0
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 9.60E-31, Aligned region: 1..182
Note	mobilization protein; binds to an origin of transfer (oriT).
Protein sequence [Download]	MAHLKKNTRGAVPGLAVHFERKTDHHTNKDIDVSKTYLNQDLMTDGSDMISRFNDRLSDV YCMKRDDVKALATWIVTLPEELAEASYEQQEAFFEETKHFLDERYGKENTMAAVVHYDET TPHLHYAFVPVVFDAKKERYKVSAKQVLTRHDLQTFHDDLDQDLKKVLPFYEKGVLNNKT LPFENVAEIKKYNDQFKALKDELTNVEENISAKKALLKITNQELDEVDLAEKQIDDFKQA LSKNLFGKTVIKPDDLDRFKTVLANMKKATLQSQRNAEQTSHELSKVKEQLADAKAEHQN LKISSATSG

Reference

[1] Chae HS et al (2013) Use of a novel Escherichia coli-leuconostoc shuttle vector for metabolic engineering of Leuconostoc citreum to overproduce D-lactate. Appl Environ Microbiol. 79(5):1428-35. [PMID:23241984]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	179	_

N/A

Information of plasmid

ID	119
Plasmid name	pMBLT00
GenBank accession number	JN106352.1
Genome size	20721 bp
Coordinate of oriT [Strand]	17020..17039 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Leuconostoc mesenteroides subsp. mesenteroides strain KCTC 13302 [33967]

Reference
N/A