 oriTDB accession number | 100124 |
| Name | oriT_pMBLT00  |
Sequence Completeness  | incomplete |
| oriT length | 20 nt |
| oriT sequence | CTCGAAAAAGGTTTTACGAG |
| IR (inverted repeat) | [1-9] [12-20] nt (CTCGTAAAA..TTTTTCGAG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site | _ |
| Note | |
Visualization of oriT structure Reference| [1] Chae HS et al (2013) Use of a novel Escherichia coli-leuconostoc shuttle vector for metabolic engineering of Leuconostoc citreum to overproduce D-lactate. Appl Environ Microbiol. 79(5):1428-35. [PMID:23241984] |
| ID | 119 |
| Name | MobA_pMBLT00 |
| GenBank accession number | AFJ91158 |
| Family | MOBV |
| Length | 309 aa |
| UniProt ID | I6Q9D0 |
| PDB ID | _ |
| Pfam | Mob_Pre [PF01076.18], Evalue: 9.60E-31, Aligned region: 1..182 |
| Note | mobilization protein; binds to an origin of transfer (oriT). |
| Protein sequence [Download] | MAHLKKNTRGAVPGLAVHFERKTDHHTNKDIDVSKTYLNQDLMTDGSDMISRFNDRLSDV YCMKRDDVKALATWIVTLPEELAEASYEQQEAFFEETKHFLDERYGKENTMAAVVHYDET TPHLHYAFVPVVFDAKKERYKVSAKQVLTRHDLQTFHDDLDQDLKKVLPFYEKGVLNNKT LPFENVAEIKKYNDQFKALKDELTNVEENISAKKALLKITNQELDEVDLAEKQIDDFKQA LSKNLFGKTVIKPDDLDRFKTVLANMKKATLQSQRNAEQTSHELSKVKEQLADAKAEHQN LKISSATSG |
Reference| [1] Chae HS et al (2013) Use of a novel Escherichia coli-leuconostoc shuttle vector for metabolic engineering of Leuconostoc citreum to overproduce D-lactate. Appl Environ Microbiol. 79(5):1428-35. [PMID:23241984] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 179 | _ |
| ID | 119 |
| Plasmid name | pMBLT00 |
| GenBank accession number | JN106352.1 |
| Genome size | 20721 bp |
| Coordinate of oriT [Strand] | 17020..17039 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Leuconostoc mesenteroides subsp. mesenteroides strain KCTC 13302 [33967] |
Reference