oriTDB
The information of the oriT region
oriTDB accession number 100123
Name oriT_pRSB105  insolico
Sequence Completeness intact
oriT length 311 nt
oriT sequence CGTGTCTCCTTCTAGCCGGGGAGCGAGGGGGGCGGGGTGTCTTGGAAAGTAAGAAGCGAA GCGCCGCACTGTCCGGGGTCAAGGGGCTTGCCCCTTGCAGGATGAGCCAGACCGTGAGAA GCGAAGCGCCGAATGGTCTGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACA ACGGCCATCCTGTTAAGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGA CGCACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAG GAGTTAGCACG
IR (inverted repeat)IR1:[80-87] [90-97] nt  (CAAGGGGC..GCCCCTTG)
IR2: [155-162] [166-173] nt  (CACGTGGC..GCCATGTG)
Location of nic site [192-193] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note
L:80-87;R:90-97;L:155-162;R:166-173;D:192-193
Visualization of oriT structure
Reference
[1] Schlüter A et al (2007) Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function. Appl Environ Microbiol. 73(6):1952-60. [PMID:17261525]
I. Information of Relaxase
ID 118
Name MobA_pRSB105 insolico
GenBank accession number ABI20469
Family MOBP
Length 889 aa
UniProt ID A1Y2V1
PDB ID _
Pfam RepB_primase [PF16793.4], Evalue: 4.00E-70, Aligned region: 547..806
Relaxase [PF03432.13], Evalue: 1.40E-30, Aligned region: 14..273
Note relaxase
Protein sequence [Download] MIVKKVPNSKTKHKTGSIRDLADYIREPHNQNPDEKVLYANGRGFLCDGHAAQREEMVAL
AAESVRSKNPVNHYIMSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHL
HLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHI
DKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPIIKKAQTWEQLHRELAAKGMRYEKTG
SGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQRQQVAQREPEPIKPDVPGWKDY
ITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKGEVLNAMRSVIA
AEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGA
SGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAAL
QLSAQKWGSFTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSE
QLKQFELYAEAVGAERYRVTSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRL
QRRGENLYYTPLSDKKHHILIDDMNREKLERLIRDGYRPAVVLESSPGNYQAIITVPKLG
TAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHQREDGSYPEVRLLKAER
RECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAYQRHYRDVIKR
QRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARYA
YSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR
Reference
[1] Schlüter A et al (2007) Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function. Appl Environ Microbiol. 73(6):1952-60. [PMID:17261525]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 177 MobB_pRSB105 insolico ABI20468 103 aa MobB; plasmid mobilization
2 178 MobC_pRSB105 insolico ABI20467 118 aa MobC; plasmid mobilization
 N/A
Information of plasmid
ID 118
Plasmid name pRSB105
GenBank accession number DQ839391.1
Incompatibility group IncP(6)
Genome size 57137 bp
Coordinate of oriT  [Strand] 26859..27169 [-]
Drug resistance experimental erythromycin resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Uncultured bacterium [77133]
Reference
[1] Schlüter A et al (2007) Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function. Appl Environ Microbiol. 73(6):1952-60. [PMID:17261525]

Microbial Bioinformatics Group in MML, SJTU