oriTDB

The information of the oriT region

oriTDB accession number	100123
Name	oriT_pRSB105
Sequence Completeness	intact
oriT length	311 nt
oriT sequence	CGTGTCTCCTTCTAGCCGGGGAGCGAGGGGGGCGGGGTGTCTTGGAAAGTAAGAAGCGAA GCGCCGCACTGTCCGGGGTCAAGGGGCTTGCCCCTTGCAGGATGAGCCAGACCGTGAGAA GCGAAGCGCCGAATGGTCTGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACA ACGGCCATCCTGTTAAGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGA CGCACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAG GAGTTAGCACG
IR (inverted repeat)	IR1:[80-87] [90-97] nt (CAAGGGGC..GCCCCTTG) IR2: [155-162] [166-173] nt (CACGTGGC..GCCATGTG)
Location of nic site	[192-193] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note
	L:80-87;R:90-97;L:155-162;R:166-173;D:192-193

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGTGTCTCCTTCTAGCCGGGGAGCGAGGGGGGCGGGGTGTCTTGGAAAGTAAGAAGCGAA

61

GCGCCGCACTGTCCGGGGTCAAGGGGCTTGCCCCTTGCAGGATGAGCCAGACCGTGAGAA

121

GCGAAGCGCCGAATGGTCTGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACA

181

ACGGCCATCCTGTTAAGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGA

241

CGCACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAG

301

GAGTTAGCACG

Reference

[1] Schlüter A et al (2007) Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function. Appl Environ Microbiol. 73(6):1952-60. [PMID:17261525]

I. Information of Relaxase

ID	118
Name	MobA_pRSB105
GenBank accession number	ABI20469
Family	MOB_P
Length	889 aa
UniProt ID	A1Y2V1
PDB ID	_
Pfam	RepB_primase [PF16793.4], Evalue: 4.00E-70, Aligned region: 547..806 Relaxase [PF03432.13], Evalue: 1.40E-30, Aligned region: 14..273
Note	relaxase
Protein sequence [Download]	MIVKKVPNSKTKHKTGSIRDLADYIREPHNQNPDEKVLYANGRGFLCDGHAAQREEMVAL AAESVRSKNPVNHYIMSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHL HLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHI DKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPIIKKAQTWEQLHRELAAKGMRYEKTG SGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQRQQVAQREPEPIKPDVPGWKDY ITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKGEVLNAMRSVIA AEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGA SGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAAL QLSAQKWGSFTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSE QLKQFELYAEAVGAERYRVTSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRL QRRGENLYYTPLSDKKHHILIDDMNREKLERLIRDGYRPAVVLESSPGNYQAIITVPKLG TAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHQREDGSYPEVRLLKAER RECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAYQRHYRDVIKR QRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARYA YSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR

Reference

[1] Schlüter A et al (2007) Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function. Appl Environ Microbiol. 73(6):1952-60. [PMID:17261525]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	177	MobB_pRSB105	ABI20468	103 aa	MobB; plasmid mobilization
2	178	MobC_pRSB105	ABI20467	118 aa	MobC; plasmid mobilization

N/A

Information of plasmid

ID	118
Plasmid name	pRSB105
GenBank accession number	DQ839391.1
Incompatibility group	IncP(6)
Genome size	57137 bp
Coordinate of oriT [Strand]	26859..27169 [-]
Drug resistance	erythromycin resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Uncultured bacterium [77133]

Reference

[1] Schlüter A et al (2007) Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function. Appl Environ Microbiol. 73(6):1952-60. [PMID:17261525]