oriTDB
The information of the oriT region
oriTDB accession number 100120
Name oriT_pHHV35  insolico
Sequence Completeness incomplete
oriT length 8 nt
oriT sequence TATCCTGC
IR (inverted repeat)_
Location of nic site [7-8] nt
Conserved sequence flanking the
  nic site 		TATCCTG|C
Note
D:7-8
Visualization of oriT structure
Reference
[1] Heuer H et al (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content. Environ Microbiol. 11(4):937-49. [PMID:19055690]
I. Information of Relaxase
ID 115
Name TraR_pHHV35 insolico
GenBank accession number YP_006960752
Family Other
Length 357 aa
UniProt ID B5TTR4
PDB ID _
Pfam
Note relaxase
Protein sequence [Download] MANGSSKTTREVQSTLLAHGEQLLNGSKVRRVNQTQSLDTFLNDLSKPKNGSGHSAKSAP
KSSPNSVKATLDRISKKTPEVMVKVTGGGNSMGKVKAHMGYITRNGQLEGIDQDGNKVNG
KDDIEDTAEDWQMSGTPISDEESKYKQAFNIVLSMPKGTDEKGVYDAAKEFAEEHFKDHK
YMMVQHTFTNDPSKDPSENPHVHVVVKAVSEKGDRLNIRKADLQKWREGFAEKLRSRGIE
ANATKRIARLQKNRSDKQSVKQMKEQGKSFKRYGKNKASPERVQKAKQQEKEALTHYKEI
TKALSQSPDPQDRKLAVDLVDYLRKQVEVKKPTPQPQIQPNKLDLQHGKNKGKEQDR
Reference
[1] Heuer H et al (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content. Environ Microbiol. 11(4):937-49. [PMID:19055690]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 173 _ insolico
Information of T4CP
ID 115
Name TraN_pHHV35 insolico
SecReT4 accesion number _
GenBank accession number YP_006960730
Family VirD4/TraG
Length 773
UniProt ID B5TTP2
PDB ID _
Pfam T4SS-DNA_transf [PF02534.13], Evalue: 7.50E-99, Aligned region: 2..447
TraG-D_C [PF12696.6], Evalue: 2.00E-22, Aligned region: 282..405
TrwB_AAD_bind [PF10412.8], Evalue: 4.20E-14, Aligned region: 21..420
Note coupling of DNA transfer and mating pair formation
Protein sequence [Download] MGNKGILVGKYHNKYLMLGGQQFVLLAAPTRSGKGVAIVIPNLLNYSDSVVVLDLKLENF
LLTSKFRAKNGQKVYLFSPFSEDLKSHKWNPFDTVSHDENFRVGEILAIGRSFYPVTGDA
KTDFWNDNANNLFLGLALYLFETEDLPVTMGEVLRQSSGKGKPIQDHIRSIIDERAQSDK
PLSDTCLDALNRFLSSPDNTLGNIISTFTSPLTIFANPIVDAATSSSDFKLEDVRRQRMS
IYFGVQPNRLGDAKNLINLFYSLLIDVNLKTLPQQDSSLKYQCLVVNDEATAVGKVAKIA
DANSFIAGYNIRLLTIIQSIGQLEDVYKTQTRGLVTNHALQIVYPPREQRDANEYSEMLG
YFTFKAKSKGQSRSMAWGQGGSNSENESDQRRALMLPQELKELSQDKEIIFMENTKPIMC
EKAKYFNDSAFVDRLKSVSPSMKAIKGFPTHKQLEKIAFEDLELSIPIPDLDVKLHKAKI
EKRITEITTVEQAEQIDISSLVIPEFKLDKTVFEDPENPQPEECLQVVDQFFGAVEWEED
TEAQNIVDDFFSQVEASAENPIENLVAVDEPEQTENLIAVDDTEFSHDEMENLVAVDEPE
QAENLVAVDEPEQVENLVAADESDPLAMIAEMENLVAVDEPEQAENLVAVDEPEQAENLV
AVDEPEQVENLVAADESDPLAMIAEMENLVAVDEPEQAENLVAVDEPEQVENLVAADESD
PLAMIAEMENLVAVDEPEQAENLVAADEGQNGSIENSDIDDVYQAFSDQVNSK

Reference
[1] Heuer H et al (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content. Environ Microbiol. 11(4):937-49. [PMID:19055690]
Information of plasmid
ID 115
Plasmid name pHHV35
GenBank accession number NC_019218.1
Genome size 59113 bp
Coordinate of oriT  [Strand]
Drug resistance insolico resistance to sulfonamide, streptomycin and gentamicin
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Uncultured bacterium HHV35 [558689]
Reference
[1] Heuer H et al (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content. Environ Microbiol. 11(4):937-49. [PMID:19055690]

Microbial Bioinformatics Group in MML, SJTU