oriTDB

The information of the oriT region

oriTDB accession number	100118
Name	oriT_pA81
Sequence Completeness	intact
oriT length	591 nt
oriT sequence	TGTCGCTACCCTTGGAGCGCGTGCGCCGTGTGTCGCGCGTAGGTCGCCACGGCTCGCCAG TAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCCGCCTCGTCGTTGGCCTGC GTGCGCAGCTCGCGCAGCACGCGGGCAAGGCGCTGCCGGTCGGCAACCGGAAGCGCCTGG AGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCT CGCTTCTTGGCCGGCGGTAGCCGGCAGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCA GGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCCCGCCGGTTAGCTAACTTCGCACA TCCTGCCCGCCTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATC TGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGA AAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCAC GGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA
IR (inverted repeat)	[148-160] [166-178] nt (AGGCGCTGCCGGT..ACCGGAAGCGCCT) [250-255] [260-265] nt (GCCGGC..GCCGGC) [318-336] [339-357] nt (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC)
Location of nic site	[365-366] nt
Conserved sequence flanking the nic site	CATCCTG\|C
Note
	L:148-160;R:166-178;L:250-255;R:260-265;L:318-336;R:339-357;D:365-366

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TGTCGCTACCCTTGGAGCGCGTGCGCCGTGTGTCGCGCGTAGGTCGCCACGGCTCGCCAG

61

TAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCCGCCTCGTCGTTGGCCTGC

121

GTGCGCAGCTCGCGCAGCACGCGGGCAAGGCGCTGCCGGTCGGCAACCGGAAGCGCCTGG

181

AGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCT

241

CGCTTCTTGGCCGGCGGTAGCCGGCAGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCA

301

GGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCCCGCCGGTTAGCTAACTTCGCACA

361

TCCTGCCCGCCTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATC

421

TGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGA

481

AAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCAC

541

GGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA

Reference

[1] Jencova V et al (2008) Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8. Res Microbiol. 159(2):118-27. [PMID:18249097]

I. Information of Relaxase

ID	113
Name	TraI_pA81
GenBank accession number	YP_195891
Family	MOB_P
Length	752 aa
UniProt ID	Q5GRA2
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 6.70E-64, Aligned region: 12..252
Note	conjugal transfer relaxase TraI
Protein sequence [Download]	MIAKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAVSVQDAITEVLATQHTN TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICAGLGYGEHQRVSAVHNDTDNLHIHIA INKIHPTRNTMHEPYYSHRALAELCTALERDYGLERDNHEPRQRGAEGRAADMEQHAGVE SLVGWIKRECLDEIKGAQSWQELHQVMRDNGLELRERANGLVIEAGDGTMVKASTLARDL SKPKLEARLGPFEASPERQTRTKAKRQYQKDPIRLRVNTVELYARYKAEQQTLTATRGQA LEQARRRKDRLVEAAKRSGRLRRATIKVIGESGPNKKLLYAQASKALRDEIQAINKQYQQ DRQALYDSHSRRTWADWLKKEATHGNAEALTALRAREAAQGLKGNTIQGRGDPRPGHAPV TDNITKKGTIIFRAGLSAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQF QAQIIRAAVDSQLPITFADPALESRRLALLKKENTHERPDRTERADQRPAEHRGRAGRGP GGPGQRAATHDDAARPIAVARAGFGRPGGGRAGTAGVHAGAVHRKPDIGRIGRVPPPQSQ HRLRTLSQLGVVRIAGGSEVLLPRDVPRHLEQQGTQPDHQLRRDISRPGSGVAAAPPGLA AADKYIAERESKRSKGFDIPKHARYTAGEGGLAFEGVRNSEGQALALLKRGDEVMVMPID QATARRMSRIAVGDAVTITPKGSIKTSKGRSR

Reference

[1] Jencova V et al (2008) Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8. Res Microbiol. 159(2):118-27. [PMID:18249097]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	169	TraH_pA81	YP_195892	136 aa	conjugal transfer protein TraH
2	170	TraJ_pA81	YP_195893	123 aa	conjugal transfer relaxosome component TraJ
3	171	TraK_pA81	YP_195894	139 aa	conjugal transfer protein TraK

Information of T4CP

ID	113
Name	TraG_pA81
SecReT4 accesion number	58616681
GenBank accession number	YP_195890
Family	VirD4/TraG
Length	634
UniProt ID	Q5GRA3
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 3.10E-163, Aligned region: 121..624 TraG-D_C [PF12696.6], Evalue: 7.60E-32, Aligned region: 444..569 TrwB_AAD_bind [PF10412.8], Evalue: 4.30E-22, Aligned region: 177..575
Note	conjugal transfer coupling protein TraG
Protein sequence [Download]	MKIKMNTAVGPQFRSAKPKTPKLLPILGGVSLVGGLQAATQFFAYTFQYHASLGANLGHV YAPWSILNWSAKWYSQYPDEIMRAGSIGMVTATVGLLGVAVAKVVTSNSSKANEYLHGSA RWAEKKDIQAAGLLPRERNVLEIVTGKDAPTATGVYVGGWQDKDGNFYYLRHSGPEHVLT YAPTRSGKGVGLVVPTLLSWGASSVITDLKGELWALTAGWRQKHAKNKVLRFEPASSSGG VCWNPLDEIRVGTEAEVGDVQNLATLIVDPDGKGLDSHWQKTAFALLVGVILHALYKARN DGGTATLPSVDAMLADPNRDIGELWMEMTTYGHVDGQNHHAIGSAARDMMDRPEEEAGSV LSTAKSYLALYRDPVVARNVSRSDFRIKDLMHSDDPVSLYIVTQPNDKARLRPLVRVMVN MIVRLLADKMDFENGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQ DINQLRSRETGYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRT AAMLGQVSRTYQEVQRPLLTPDECLRMPGPKKNDKGEIEEAGDMVIYVAGYPAIYGKQPL YFKDPVFQARASIPAPKATDRLRQVVEAGEGITI

Reference

[1] Jencova V et al (2008) Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8. Res Microbiol. 159(2):118-27. [PMID:18249097]

Information of plasmid

ID	113
Plasmid name	pA81
GenBank accession number	NC_006830.1
Incompatibility group	IncP1
Genome size	98192 bp
Coordinate of oriT [Strand]	68359..68949 [+]
Drug resistance	_
Heavy-metal resistance	mercury resistance
Virulence factor	_
Xenobiotic degradation	genes encoding (halo)aromatic compound degradation
Host bacterium [NCBI Taxonomy ID]	Achromobacter xylosoxidans A8 [762376]

Reference

[1] Jencova V et al (2008) Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8. Res Microbiol. 159(2):118-27. [PMID:18249097]