oriTDB
The information of the oriT region
oriTDB accession number 100118
Name oriT_pA81  insolico
Sequence Completeness intact
oriT length 591 nt
oriT sequence TGTCGCTACCCTTGGAGCGCGTGCGCCGTGTGTCGCGCGTAGGTCGCCACGGCTCGCCAG TAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCCGCCTCGTCGTTGGCCTGC GTGCGCAGCTCGCGCAGCACGCGGGCAAGGCGCTGCCGGTCGGCAACCGGAAGCGCCTGG AGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCT CGCTTCTTGGCCGGCGGTAGCCGGCAGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCA GGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCCCGCCGGTTAGCTAACTTCGCACA TCCTGCCCGCCTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATC TGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGA AAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCAC GGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA
IR (inverted repeat)[148-160] [166-178] nt  (AGGCGCTGCCGGT..ACCGGAAGCGCCT)
[250-255] [260-265] nt  (GCCGGC..GCCGGC)
[318-336] [339-357] nt  (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC)
Location of nic site [365-366] nt
Conserved sequence flanking the
  nic site 		CATCCTG|C
Note
L:148-160;R:166-178;L:250-255;R:260-265;L:318-336;R:339-357;D:365-366
Visualization of oriT structure
Reference
[1] Jencova V et al (2008) Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8. Res Microbiol. 159(2):118-27. [PMID:18249097]
I. Information of Relaxase
ID 113
Name TraI_pA81 insolico
GenBank accession number YP_195891
Family MOBP
Length 752 aa
UniProt ID Q5GRA2
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 6.70E-64, Aligned region: 12..252
Note conjugal transfer relaxase TraI
Protein sequence [Download] MIAKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAVSVQDAITEVLATQHTN
TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICAGLGYGEHQRVSAVHNDTDNLHIHIA
INKIHPTRNTMHEPYYSHRALAELCTALERDYGLERDNHEPRQRGAEGRAADMEQHAGVE
SLVGWIKRECLDEIKGAQSWQELHQVMRDNGLELRERANGLVIEAGDGTMVKASTLARDL
SKPKLEARLGPFEASPERQTRTKAKRQYQKDPIRLRVNTVELYARYKAEQQTLTATRGQA
LEQARRRKDRLVEAAKRSGRLRRATIKVIGESGPNKKLLYAQASKALRDEIQAINKQYQQ
DRQALYDSHSRRTWADWLKKEATHGNAEALTALRAREAAQGLKGNTIQGRGDPRPGHAPV
TDNITKKGTIIFRAGLSAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQF
QAQIIRAAVDSQLPITFADPALESRRLALLKKENTHERPDRTERADQRPAEHRGRAGRGP
GGPGQRAATHDDAARPIAVARAGFGRPGGGRAGTAGVHAGAVHRKPDIGRIGRVPPPQSQ
HRLRTLSQLGVVRIAGGSEVLLPRDVPRHLEQQGTQPDHQLRRDISRPGSGVAAAPPGLA
AADKYIAERESKRSKGFDIPKHARYTAGEGGLAFEGVRNSEGQALALLKRGDEVMVMPID
QATARRMSRIAVGDAVTITPKGSIKTSKGRSR
Reference
[1] Jencova V et al (2008) Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8. Res Microbiol. 159(2):118-27. [PMID:18249097]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 169 TraH_pA81 insolico YP_195892 136 aa conjugal transfer protein TraH
2 170 TraJ_pA81 insolico YP_195893 123 aa conjugal transfer relaxosome component TraJ
3 171 TraK_pA81 insolico YP_195894 139 aa conjugal transfer protein TraK
Information of T4CP
ID 113
Name TraG_pA81 insolico
SecReT4 accesion number 58616681
GenBank accession number YP_195890
Family VirD4/TraG
Length 634
UniProt ID Q5GRA3
PDB ID _
Pfam T4SS-DNA_transf [PF02534.13], Evalue: 3.10E-163, Aligned region: 121..624
TraG-D_C [PF12696.6], Evalue: 7.60E-32, Aligned region: 444..569
TrwB_AAD_bind [PF10412.8], Evalue: 4.30E-22, Aligned region: 177..575
Note conjugal transfer coupling protein TraG
Protein sequence [Download] MKIKMNTAVGPQFRSAKPKTPKLLPILGGVSLVGGLQAATQFFAYTFQYHASLGANLGHV
YAPWSILNWSAKWYSQYPDEIMRAGSIGMVTATVGLLGVAVAKVVTSNSSKANEYLHGSA
RWAEKKDIQAAGLLPRERNVLEIVTGKDAPTATGVYVGGWQDKDGNFYYLRHSGPEHVLT
YAPTRSGKGVGLVVPTLLSWGASSVITDLKGELWALTAGWRQKHAKNKVLRFEPASSSGG
VCWNPLDEIRVGTEAEVGDVQNLATLIVDPDGKGLDSHWQKTAFALLVGVILHALYKARN
DGGTATLPSVDAMLADPNRDIGELWMEMTTYGHVDGQNHHAIGSAARDMMDRPEEEAGSV
LSTAKSYLALYRDPVVARNVSRSDFRIKDLMHSDDPVSLYIVTQPNDKARLRPLVRVMVN
MIVRLLADKMDFENGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQ
DINQLRSRETGYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRT
AAMLGQVSRTYQEVQRPLLTPDECLRMPGPKKNDKGEIEEAGDMVIYVAGYPAIYGKQPL
YFKDPVFQARASIPAPKATDRLRQVVEAGEGITI

Reference
[1] Jencova V et al (2008) Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8. Res Microbiol. 159(2):118-27. [PMID:18249097]
Information of plasmid
ID 113
Plasmid name pA81
GenBank accession number NC_006830.1
Incompatibility group IncP1
Genome size 98192 bp
Coordinate of oriT  [Strand] 68359..68949 [+]
Drug resistance _
Heavy-metal resistance insolico mercury resistance
Virulence factor _
Xenobiotic degradation insolico genes encoding (halo)aromatic compound degradation
Host bacterium [NCBI Taxonomy ID] Achromobacter xylosoxidans A8 [762376]
Reference
[1] Jencova V et al (2008) Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8. Res Microbiol. 159(2):118-27. [PMID:18249097]

Microbial Bioinformatics Group in MML, SJTU